FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63968
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1031016.11743371685843No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG61889.6735867933967No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG21973.4345297648824413No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG15992.4996873436718356No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG14592.2808279139569785No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG9851.539832416208104No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG9251.4460355177588793No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG8461.322536268134067No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG7961.2443721860930466No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG7341.1474487243621811No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG7341.1474487243621811No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG5930.9270260130065032No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG5350.8363556778389194No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG4900.7660080040020011No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA4750.7425587793896948No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA4510.705040020010005No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3540.5534017008504253No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG3500.5471485742871436No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG3380.5283891945972986No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG3370.5268259129564783No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3000.4689844922461231No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCTACTTCACGCGTTAGCTGCG2760.43146573286643325No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG2660.4158329164582291No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG2490.38925712856428213No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG2050.3204727363681841No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG2030.3173461730865433No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG1890.2954602301150575No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1880.29389694847423714No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCTACTTCACGCGTTAGCTGCG1840.2876438219109555No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAATGCGTTAGCTGCG1630.25481490745372687No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1580.2469984992496248No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTTCG1520.23761880940470237No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAATTTCACGCGTTAGCTACG1490.23292896448224112No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1450.22667583791895948No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1420.22198599299649824No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1400.21885942971485742No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG1400.21885942971485742No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1390.217296148074037No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1340.20947973986993498No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATGCTTATTGCGTTAACTGCA1290.20166333166583292No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG1290.20166333166583292No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG1280.2001000500250125No Hit
CAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1260.19697348674337167No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAACGCGTTAGCTGCG1240.19384692346173085No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCATGCGTTAGCTGCG1210.18915707853926964No Hit
CAACCTTGCGGCCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTTCG1170.182903951975988No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTCTCGCGTTAGCTACG1150.17977738869434717No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGCTGCG1120.17508754377188596No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG1100.17196098049024514No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA1090.1703976988494247No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG1070.1672711355677839No Hit
CAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1060.16570785392696347No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTCGCTGCG1060.16570785392696347No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG1000.156328164082041No Hit
AACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG970.1516383191595798No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG960.1500750375187594No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCTCGCGTTAGCTGCG960.1500750375187594No Hit
CAACCTTGCGGCCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTTCG930.14538519259629815No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG920.14382191095547775No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG900.1406953476738369No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG890.1391320660330165No Hit
TAACCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG830.12975237618809407No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG800.12506253126563283No Hit
TAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG740.11568284142071035No Hit
TAACCTTGCGGCCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTGCG740.11568284142071035No Hit
TAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG700.10942971485742871No Hit
TAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG650.10161330665332666No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG640.10005002501250625No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG640.10005002501250625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGG450.0252.296789
CGACCCT151.3453222E-5252.296789
AGTGCGG151.3453222E-5252.296785
AAACCTT1850.0252.296781
TTGCGGA151.3453222E-5252.296786
TTGCGAT1050.0252.296786
ATGCGAG151.3453222E-5252.296786
TTGCCGG1850.0252.296786
CGATCGT1050.0252.296789
CGAACGT2700.0252.296779
GCGACCG18900.0252.296778
CAACCTT15450.0252.296771
AACATTG251.3387762E-9252.296772
TGCGAGC1000.0252.296777
TGCGAAC2800.0252.296777
TTGCGGT700.0252.296776
GTGCGGC500.0252.296776
GTTGCCG1950.0252.296775
CGGACGT251.3387762E-9252.296779
TCTTGAG100.0013489532252.296754
Produced by FastQC (version 0.11.5)