FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339420
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG6400018.85569500913323No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG251327.404395733899004No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG119793.529255789287608No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG59511.7532850156148725No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG58641.7276530552118319No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT56641.6687290083082906No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA56491.664309704790525No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG55361.6310176182900242No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG37231.0968711331094219No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG29700.8750220965175889No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG24620.7253550173825938No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCTACTTCACGCGTTAGCTGCG24410.719167992457722No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG21790.6419774910140829No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG21480.6328442637440339No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG20070.5913028106770373No Hit
CACCCTTGCGAGCATACTTCCCAGGCGGGATACTTAACGCGTTAGCTACG19680.5798126215308467No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG19400.571563254964351No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA18240.537387307760297No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG17940.5285487007247658No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG17940.5285487007247658No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG17470.5147015497024335No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCTACTTCACGCGTTAGCTGCG17040.5020328796181721No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG15610.4599021860821401No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG15260.44959047787402034No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCATGCGTTAGCTGCG12290.3620882682222615No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG11880.3500088386070355No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG11610.34205409227505745No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG11560.3405809911024689No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA11540.3399917506334335No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGCTGCG11460.33763478875729186No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTCGCTGCG11340.3340993459430794No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG11020.3246714984385128No Hit
CACCCTTGCGAGCATACTTCCCAGGCGGGGTACTTAACGCGTTAGCTACG10710.3155382711684639No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG10540.31052972718166283No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCTCGCGTTAGCTGCG10460.3081727653055212No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG9950.2931471333451181No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG9220.27163985622532555No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG8370.24659713629132052No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG8080.23805314949030698No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG7850.23127688409639974No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG7340.2162512521359967No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG7290.21477815096340816No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG6790.20004713923752285No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG5790.17058511578575217No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCTTTAGCTGCG5770.16999587531671675No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATGCTTATTGCGTTAACTGCA5230.1540863826527606No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG5200.15320252194920747No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGCTGACTTAACGCGTTAGCTACG5020.14789935772788876No Hit
TAATCTTGCGACCGTACTCCCCCGGCGGTCTACTTCACGCGTTAGCTGCG5010.14760473749337105No Hit
AAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT4760.14023923163042837No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTCCG4520.13316834600200342No Hit
TAACCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG4400.12963290318779094No Hit
TAATCTTGCGACCGTACTCCCCAGGTGGTCTACTTCACGCGTTAGCTGCG4360.12845442224972012No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG4340.1278651817806847No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAACGCGTTAGCTACG4260.12550821990454303No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA4160.12256201755936598No Hit
TAATCTTGCGACCGTACTCCCCAGGCTGTCTACTTCACGCGTTAGCTGCG4160.12256201755936598No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG4050.1193211949796712No Hit
TAATCTTGCGACCGTCCTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG3990.11755347357256497No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG3830.11283954982028166No Hit
CAATCTTGCGATCGTACTCCCCAGGCGGAGTGCTTAACGTGTTAACTTTA3690.10871486653703376No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG3630.10694714512992753No Hit
CAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3530.10400094278475046No Hit
CAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG3470.10223322137764422No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3460.10193860114312651No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3440.1013493606740911No Hit
TAATCGTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG3430.10105474043957338No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAATGCGTTAGCTGCG3430.10105474043957338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCCG201.3564022E-7251.970578
GCCGGCG5400.0251.970578
AGACCAT100.0013568376251.970579
TTGCCGG5350.0251.970576
CTAGCGA151.3575809E-5251.970575
AAGTCGT100.0013568376251.970571
AGACCGG550.0251.970559
CGGACGT1250.0251.970559
GCGACCG177750.0251.403538
CTTGCGA203300.0250.793155
TGCGACC188300.0250.699337
CGACCGT174750.0250.456579
GCGGCCG65500.0250.431828
TTGCGAC183700.0250.32466
ATCTTGC158650.0250.302933
TTGCGAA7500.0250.290766
TGCGGCC65750.0250.054447
AGTCTTG12850.0250.00972
CGGCCGT64500.0249.821989
AACCTTG71850.0249.515732
Produced by FastQC (version 0.11.5)