FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528985
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG5543110.478747034414965No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG306315.7905233607758255No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG243644.605801676796128No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG126002.3819200922521433No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG75591.428963014074123No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTACG74551.4093027212491849No Hit
CACTCTTGCGAGCATACTCCCCAGGCGGAGTACTTAATGCGTTTGCTTCA62951.1900148397402575No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG54181.0242256396684217No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTGCG51380.9712940820628185No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG50510.9548474909496488No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG46170.8728035766609639No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGAAGCTTAATGCGTTAGCTGCG40480.7652390899552918No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTCTCGCGTTAGCTACG40260.7610801818577086No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCG40040.7569212737601255No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG39090.738962352429653No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTACTTAATGCGTTAGCTACG34540.6529485713205478No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG32110.6070115409699708No Hit
CAGCCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCA28050.5302607824418462No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA26380.4986908891556471No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAGCGCCG26220.4956662287210412No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGCGTTAGCTGCG25740.4865922474172235No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG25720.4862141648628978No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT24760.4680662022552624No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTACG22950.4338497310887832No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAGAACTTATTGCGTTAACTGCG22310.42175108935035965No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG22120.41815930508426513No Hit
CATTCTTGCGAACGTACTACTCAGGCGGGTCACTTATTGCGTTGACTGCA20960.3962305169333724No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG20440.3864003705209032No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG19840.37505789389113114No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATACTTAATGCGTTAGCGGCG19630.37108802707071087No Hit
CAATCTTGCGATCGTACTCCCCAGGCGGAATGCTTATCGTGTTTACGTCG18990.3589893853322873No Hit
TAGTNTTGNGACNGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG18510.3499154040284696No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG17000.3213701711768765No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG16790.3174003043564562No Hit
CAGTCTTGCGACCGTACTTCCCAGGCGGCGGACTTAACAGCTTCCCTTCG15360.2903674017221661No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTATTGTGTTAACTGCG14910.281860544249837No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCA14260.2695728612342505No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGATACTTATTGCGTTAACTCCG13750.2599317560989442No Hit
TAGTCTTGCGACCGTACTCCCCAGTCGGTCTACTTATCGCGTTAGCTGCG13670.25841942588164124No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG13300.25142489862661516No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGATGCTTAACGCGTTGGCTCCG13100.24764407308335776No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG13090.2474550318061949No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA12990.24556461903456622No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTACTGCGTTTGCGACG11730.2217454181120448No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG11370.2149399321341815No Hit
CAACCTTGCGGTCGTACTCCCCAGGCGGAGTGCTTAATGCGTTTGCTGCG11220.21210431297673848No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGGTACTTAGTGTGTTAACGACG11140.21059198275943553No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA10540.19924950612966338No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG10500.19849334102101193No Hit
CAATCTTGCGATCGTACTCCCCAGGTGGAATACTTATTGTGTTAACTGCG10410.19679196952654612No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG10360.19584676314073177No Hit
CAGCCTTGCGACCATACTCCCCCCAGAACCCAAAGACTTTGATTTCTCAT9930.18771798822272845No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATCACTTAGCGCGTTAGCTACG9850.1862056580054255No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG9780.1848823690652854No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG9610.18166866735351664No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG9240.1746741400984905No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGGGTACTTATTGCGTTAGCTCCG9150.1729727686040247No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTATTGCGTTTGCTGCG9140.1727837273268618No Hit
CATTCTTGCGAACGTACTCCCCAGGCGGAGCACTTACTGCGTTAACTGCG8410.15898371409397244No Hit
TAACCTTGCGGTCGTACTCCCCAGGCGGAGAACTTATCGCGTTAGCTTCG8330.15747138387666948No Hit
CAACCTTGCGGTCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG8310.15709330132234373No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGACACTTAATGCGTTAGCTTCG8140.15387959961057496No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG7930.14990973279015474No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG7710.1457508246925716No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGTGTTTAATGCGTTAGCTGCG7710.1457508246925716No Hit
CAGGCTTGCGCCCGTACTCCCCAGGTGGCTGACTTATCACTTTCGCTTGG7630.14423849447526868No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGCACGCTTAACGCGTTTGCTCCG7570.14310424681229145No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTAATGCGTTAGCTGCG7490.14159191659498852No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTCGCTGCG7460.1410247927634999No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAGCGCGTTAGCTACG7420.14026862765484843No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG7410.14007958637768556No Hit
CAGCCTTGCGACCGTACTACTCAGGTGGGATACTTAATGTGTTAACTTCA7310.13818917360605687No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGGATACTTATTGCGTTTGCTCCG7000.13232889401400796No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTACG6860.1296823161337278No Hit
TAGTCTTGCGACCGTACTCCCCATGCGGTCTACTTATCGCGTTAGCTGCG6800.1285480684707506No Hit
CACTCTTGCGAGCATACTCCCCAGGCGGGATACTTCACGCGTTAGCTCCG6700.1266576556991219No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATAACTTTCGCTTAG6680.12627957314479615No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTACTTAAAGCGTTAGCTACG6640.1255234080361447No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATAACTTACCGCTTTCGCTAAG6640.1255234080361447No Hit
TAGCCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG6610.12495628420465608No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGATGCTTAACGCGTTAGCTCCG6580.1243891603731675No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG6530.12344395398735314No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTTAATGCGTTAGCTGCG6490.12268778887870166No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTAATGTGTTAACGCCG6420.12136449993856158No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG6420.12136449993856158No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG6150.11626038545516414No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG6090.11512613779218692No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG6090.11512613779218692No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCATCTTCACGCGTTAGCTACG6070.11474805523786118No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTGCG6040.11418093140637259No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG5970.1128576424662325No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG5900.11153435352609244No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG5870.11096722969460382No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA5770.10907681692297513No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCG5730.10832065181432367No Hit
CAATCTTGCGATCGTACTACTCAGGCGGAGTACTTAATATGTTAATTTCA5580.10548503265688063No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG5540.10472886754822916No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG5520.10435078499390342No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGTGTTTAATGCGTTTGCTGCG5420.10246037222227473No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTACG5420.10246037222227473No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGGTACTTATTGTGTTAACGACG5390.10189324839078612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAG201.3556382E-7252.026874
GCGACCG249200.0251.217798
TGCGAGC33700.0250.905097
CGGCCGT133750.0250.707849
GCGGCCG135500.0250.44598
CGACCGT245850.0250.437939
CTTGCGG138700.0249.392125
CTTGCGA306700.0249.15085
TGCGACC260700.0249.037
TCTTGCG294250.0248.17264
TTGCCGG3150.0248.026466
AACCTTG75150.0247.667132
CCTTGCG155150.0247.153664
AGTCTTG96600.0247.069842
GTCTTGC96500.0246.93413
AGCCTTG77550.0246.827092
ATCTTGC152200.0246.810823
TTGCGAC256300.0246.569416
TGCGAAC15150.0246.204477
ACCTTGC75850.0245.713753
Produced by FastQC (version 0.11.5)