FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265048
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG262679.910280402040385No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG178366.729347137122332No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG112874.258473936796354No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG86243.2537502641031057No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA82563.1149074884549215No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG74582.813829947783043No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG62352.352404092843561No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT56092.1162204581811594No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG34271.292973348223718No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG33781.2744861308140412No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG31571.191105007394887No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG28841.0881047961124022No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG21790.822115239503788No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG20580.776463131206423No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA17410.656862153270351No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG16690.6296972623826628No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA15340.5787630919682473No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG13460.5078325435392834No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG13210.49840028975883616No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG13090.4938728079442214No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG12350.4659533367540974No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG11100.4187920678518608No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCTACTTCACGCGTTAGCTGCG10230.3859678246959041No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG9690.36559415653013794No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA8670.3271105611059129No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG8270.3120189550571972No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG8200.3093779239986719No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG7650.28862696568168783No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG7370.27806284144758686No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG7040.2656122664573964No Hit
AACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG6070.22901512178926084No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG6010.22675138088195346No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCATGCGTTAGCTGCG6010.22675138088195346No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG5960.224864930125864No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG5730.21618725664785246No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCTACTTCACGCGTTAGCTGCG5270.19883190969182943No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTCGCTGCG5260.1984546195406115No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG4820.18185385288702421No Hit
AAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT4680.17657179076997373No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG4600.1735534695602306No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG4540.17128972865292325No Hit
CACCCTTGCGAGCATACTTCCCAGGCGGGGTACTTAACGCGTTAGCTACG4540.17128972865292325No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG4470.168648697594398No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG4420.16676224683830854No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGCTGCG4370.16487579608221908No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAATGCGTTAGCTGCG4310.1626120551749117No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG4230.15959373396516857No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG4040.1524252210920286No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCTCGCGTTAGCTGCG3940.14865231957984967No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG3750.1414838067067097No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG3720.14035193625305606No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG3600.13582445443844135No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3480.13129697262382664No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG3370.1271467809604298No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG3200.12073284838972564No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG3170.11960097793607197No Hit
CACCCTTGCGAGCATACTTCCCAGGCGGGATACTTAACGCGTTAGCTACG3170.11960097793607197No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3130.11809181733120039No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG3000.11318704536536779No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG2920.11016872415562465No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGAACTTAATGCGTTAGCTGCG2900.10941414385318886No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTACG2870.10828227339953517No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG2870.10828227339953517No Hit
TAACCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG2800.10564124234100994No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG2690.10149105067761312No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATGCTTATTGCGTTAACTGCA2690.10149105067761312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAAC5500.0252.22187
CTTGTGA100.0013526223252.221795
TTGTGAC100.0013526223252.221796
AGACGGG100.0013526223252.221799
GAGACGG100.0013526223252.221798
GTGAGGC201.349108E-7252.221796
GTGAGAC201.349108E-7252.221796
GGTTCAG100.0013526223252.221799
GTGCGAG201.349108E-7252.221796
AGTCGTG251.3533281E-9252.221792
GTGCGGC450.0252.221776
CGACCGT122850.0251.400569
GCGACCG125100.0251.314518
TGCGGCC51800.0251.004527
TGCGACC134050.0250.904717
ATCTTGC104350.0250.89243
CTTGCGA148500.0250.863025
TTGCGAC130650.0250.677376
AACCTTG58150.0250.269942
CGGCCGT51250.0250.253229
Produced by FastQC (version 0.11.5)