FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176402
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG132777.526558655797554No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG115956.573054727270666No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG91365.1790796022720835No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG64803.6734277389145245No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG62943.567986757519756No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG29951.6978265552544756No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTGCG24461.386605594040884No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG20671.1717554222741238No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTACTTAATGCGTTAGCTACG19211.0889899207491978No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG18231.033434995068083No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTACG17851.0118932891917325No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGAAGCTTAATGCGTTAGCTGCG16370.9279940136733145No Hit
CAACCTTGCGGCCGTACTTCCCAGGCGGAAGACTTATCGCGTTAGCTTCG11920.6757293001213138No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG11630.6592895772156778No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTACG11410.6468180632872643No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGCGTTAGCTGCG10230.5799253976712282No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG8910.5050963141007472No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCG8800.4988605571365404No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG8480.4807201732406662No Hit
CAACCTTGCGGTCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG8190.4642804503350302No Hit
CAACCTTGCGGCCGTACTTCCCAGGCGGATGACTTATCGCGTTAGCTTCG7740.4387705354814571No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG6640.37641296583938955No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATACTTAATGCGTTAGCGGCG6490.36790966088819854No Hit
CACTCTTGCGAGCATACTCCCCAGGCGGAGTACTTAATGCGTTTGCTTCA6040.3423997460346254No Hit
CAACCTTGCGGCCGTACTTCCCAGGCGGAAGACTTATCGCGTTAGCTTCA5470.31008718722009954No Hit
CAACCTTGCGGCCGTACTTCCCAGGCGGAGTACTTATCGTGTTAACTTCA5440.30838652622986135No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAGAACTTATCGCGTTAGCTTCG5160.2925136903209714No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTATCGCGTTTGCTGCG4470.25339848754549266No Hit
TAGTNTTGNGACNGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG4210.2386594256300949No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG4200.2380925386333488No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG3990.22618791170168137No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTTCG3950.2239203637146971No Hit
CAATCTTGCGATCGTACTCCCCAGGCGGAATGCTTATCGTGTTTACGTCG3920.2222197027244589No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG3870.21938526774072856No Hit
CAACCTTGCGGTCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCA3790.21485017176676No Hit
AACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG3720.21088196278953755No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTTAAAGCGTTAGCTGCG3610.20464620582533077No Hit
TAGTCTTGCGACCGTACTCCCCAGTCGGTCTACTTATCGCGTTAGCTGCG3390.19217469189691727No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3270.18537204793596446No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3110.17630185598802736No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2990.16949921202707452No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAGAACTTATTGCGTTAACTGCG2980.16893232503032846No Hit
CAACCTTGCGGCCGTACTTCCCAGGCGGAAGACTTATCGCGTTAGCTTTG2880.16326345506286777No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG2800.1587283590888992No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGATAACTTAACGCGTTAGCTTCG2780.15759458509540708No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAAAACTTATCGCGTTAGCTTCG2720.15419326311493067No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTACG2640.1496581671409621No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG2640.1496581671409621No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGAACACTTAACGCTTTCGCTACG2610.14795750615072392No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTCTCGCGTTAGCTACG2600.14739061915397786No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGAACTTCTCGCGTTTGCTTCG2520.1428555231800093No Hit
CAGTCTTGCGACCGTACTTCCCAGGCGGCGGACTTAACAGCTTCCCTTCG2410.13661976621580255No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG2410.13661976621580255No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGAGAACTTATCGCGTTAGCTTCG2360.1337853312320722No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCATCTTCACGCGTTAGCTACG2350.13321844423532614No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2310.13095089624834186No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTATTGTGTTAACTGCG2290.1298171222548497No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG2290.1298171222548497No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTTAATGCGTTAGCTGCG2200.1247151392841351No Hit
CAACCTTGCGGCCGTACTTCCCAGGCGGAAGACTTATCGTGTTAACTTCG2180.12358136529064295No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2140.12131381730365869No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGCG2110.11961315631342048No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2080.11791249532318228No Hit
CAGCCTTGCGACCGTACTACTCAGGCGGAGTACTTAATACGTTAGCTTCA2080.11791249532318228No Hit
CAGCCTTGCGACCATACTCCCCCCAGAACCCAAAGACTTTGATTTCTCAT2050.11621183433294408No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTAATGCGTTAGCTGCG2040.115644947336198No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTACG2040.115644947336198No Hit
CACTNTTGNGAGNGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG2030.11507806033945193No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGCACGCTTAACGCGTTTGCTCCG2030.11507806033945193No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTACG2010.1139442863459598No Hit
CAATCTTGCGATCGTACTCCCCAGGTGGGATACTTATTGTGTTAACTCCG1960.11110985136222945No Hit
CAACCTTGCGGCCGTACTTCCCAGGCGGAATACTTATCGCGTTAGCTTCG1960.11110985136222945No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAACGCGTTAGCTACG1920.10884230337524518No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTACTTAATGCGTTAGCTGCG1920.10884230337524518No Hit
CAACCTTGCGGTCGTACTCCCCAGGCGGGATACTTAATGCGTTAGCTCCG1900.10770852938175304No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTACCGCGTTAGCTGCG1890.10714164238500698No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG1890.10714164238500698No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGAGAACTTAACGCGTTAGCTTCG1860.10544098139476878No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTACG1850.1048740943980227No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTGCG1810.10260654641103843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGAG600.0251.837484
AAATATT500.0251.837481
ATAGCGA100.0013584179251.837485
ATAGAGC100.0013584179251.837483
TTGAGAC600.0251.837486
CTTGAGA600.0251.837485
GTGAGGC100.0013584179251.837486
GTGAGAC201.3580939E-7251.837486
TTGCCGG251.364242E-9251.837486
GATCTTG100.0013584179251.837482
GTCTTGA251.364242E-9251.837483
ATAGTGA201.3580939E-7251.837483
ATTGAGA100.0013584179251.837485
GTGCGAG201.3580939E-7251.837486
TAGTCGT251.364242E-9251.837481
GCGACCG90600.0251.281548
CGACCGT88900.0250.704329
AGTCTTG26950.0249.968552
TGCGACC92400.0249.92967
GCGGCCG42150.0249.746298
Produced by FastQC (version 0.11.5)