FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80421
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1643120.431230648711157No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG62217.735541711741958No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG24713.0725805448825554No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG20892.5975802340184777No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA19742.4545827582347894No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG17662.195943845512988No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG15341.907461981323286No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG11431.421270563658746No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG10261.2757861752527326No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA8141.012173437286281No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG8030.9984954178634934No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG6730.8368460974123674No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG6030.7498041556309919No Hit
TAGTNTTGNGACNGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG5230.6503276507379913No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG4950.6155108740254411No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTCTCGCGTTAGCTACG4690.5831810099352159No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG4580.5695029905124284No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG4320.5371731264222032No Hit
TAGTCTTGCGACCGTACTCCCCAGTCGGTCTACTTATCGCGTTAGCTGCG4310.5359296701110406No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG4240.527225475932903No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3900.48494796135337787No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3820.4750003108640778No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG3180.3954191069496773No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG3160.3929321943273523No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG3120.3879583690827023No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTCGCTGCG2890.3593588739259646No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG2630.3270290098357394No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG2510.3121075341017893No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2280.28350803894505167No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG2270.2822645826338892No Hit
TAGTCTTGCGACCGTACTCCCCATGCGGTCTACTTATCGCGTTAGCTGCG2230.27729075738923914No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG2000.24869126223250143No Hit
CAGCCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCA1780.2213352233869263No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1740.21636139814227626No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1710.21263102920878874No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1700.21138757289762625No Hit
TAGCCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1600.19895300978600117No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG1500.18651844667437611No Hit
AACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1330.16537968938461348No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG1320.16413623307345096No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG1310.16289277676228847No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1310.16289277676228847No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1300.16164932045112596No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCT1180.14672784471717587No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATTGCGTTAGCTGCG1080.1342932816055508No Hit
TAGTCTTGCGACCGTCCTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1070.1330498252943883No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCTTTAGCTGCG1020.12683254373857575No Hit
TAGTCGTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1000.12434563111625072No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG960.11937180587160069No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG960.11937180587160069No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG920.11439798062695067No Hit
TAGTCTTGCGACCGTACTCCCCAGGTGGTCTACTTATCGCGTTAGCTGCG910.11315452431578817No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTCTCGCGTTAGCTGCG910.11315452431578817No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG910.11315452431578817No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG910.11315452431578817No Hit
TAACCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG840.10445033013765062No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG830.1032068738264881No Hit
CAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG810.1007199612041631No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTTCG810.1007199612041631No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG810.1007199612041631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA600.0252.445455
CATTGCG151.3434836E-5252.445454
GCCGGCG3500.0252.445458
TTGAGAC151.3434836E-5252.445456
GAGACAG151.3434836E-5252.445458
ATGAGAC151.3434836E-5252.445456
CTTGCAG151.3434836E-5252.445455
TGCGAAC2850.0252.445457
AGACCGT151.3434836E-5252.445459
CTTGAGA151.3434836E-5252.445455
TTGCAGC151.3434836E-5252.445456
ATTGCGG151.3434836E-5252.445455
AACAGTG151.3434836E-5252.445452
TGAGACC301.2732926E-11252.445457
AAGTCGT151.3434836E-5252.445451
TAGAGGA100.0013472382252.445436
AATTTTG100.0013472382252.445432
AGTCAAG100.0013472382252.445432
CGGCCGA100.0013472382252.445439
CTTCAGG100.0013472382252.445435
Produced by FastQC (version 0.11.5)