FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82233
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG917311.154889156421389No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG54366.610484841851812No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG44155.368890834579791No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG25893.1483710918974137No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG19412.3603662763124293No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG16462.001629516130994No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG13541.646540926392081No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG11411.3875208249729427No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG7890.9594688263835687No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA7800.9485243150559994No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG6690.8135420086826457No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG6380.7758442474432405No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG6210.7551712816022764No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG5960.7247698612479175No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG5800.7053129522211278No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA5340.6493743387691072No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG5290.6432940546982355No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAATGCGTTAGCTGCG5200.6323495433706663No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG5150.6262692592997945No Hit
CAGCCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCA5040.6128926343438765No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG4230.5143920323957535No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG4010.4876387824839176No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3980.4839906120413946No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA3900.4742621575279997No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTCTCGCGTTAGCTACG3830.4657497598287792No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3790.46088553257208176No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG3710.4511570780586869No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3630.441428623545292No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG3530.4292680554035484No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA3400.4134593168192818No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG3010.3660331010664818No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2810.34171196478299465No Hit
TAGTNTTGNGACNGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG2790.33927985115464593No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTCCG2720.3307674534554254No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG2690.3271192830129024No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2630.3198229421278562No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAGTGCGTTAGCTGCG2520.30644631717193827No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAATTTCACGCGTTAGCTACG2280.2772609536317537No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG2160.26266827186166136No Hit
TAGTCTTGCGACCGTACTCCCCAGTCGGTCTACTTATCGCGTTAGCTGCG2080.25293981734826654No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG2020.24564347646322035No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG1800.2188902265513845No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1780.2164581129230358No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1670.2030814879671178No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1610.19578514708207168No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG1600.19456909026789732No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG1590.19335303345372296No Hit
AACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG1520.18484063575450246No Hit
AACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1500.18240852212615374No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG1480.17997640849780502No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG1480.17997640849780502No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1460.1775442948694563No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG1400.17024795398441014No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG1360.1653837267277127No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG1360.1653837267277127No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG1350.16416766991353837No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1230.14957498814344605No Hit
TAGTCTTGCGACCGTACTCCCCATGCGGTCTACTTATCGCGTTAGCTGCG1210.14714287451509733No Hit
TAGCCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1060.12890202230248196No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG1020.12403779504578455No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTACG1010.12282173823161018No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG990.12038962460326147No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTGG990.12038962460326147No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTCGCTGCG950.11552539734656403No Hit
CACTNTTGNGAGNGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG930.11309328371821531No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG910.11066117008986659No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATGCTTATTGCGTTAACTGCA910.11066117008986659No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATACTTAATGCGTTTGCTGCG900.10944511327569224No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG890.1082290564615179No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG870.10579694283316916No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTACG850.10336482920482044No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTACG850.10336482920482044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCG350.0252.127298
CGTGCGA301.2732926E-11252.127275
TTGAGAC151.3503821E-5252.127276
TGCGAGC5000.0252.127277
TGCGAAC3550.0252.127277
GTGCGAC301.2732926E-11252.127276
GAGGCCG151.3503821E-5252.127278
AACGTTG201.3458339E-7252.127262
ACGTTGC201.3458339E-7252.127263
AGGCCGG100.0013523949252.127269
AACATTG100.0013523949252.127262
ATGAGGC100.0013523949252.127266
AATTGCG100.0013523949252.127264
AGACCGG201.3458339E-7252.127269
CTTGAGA251.3478711E-9252.127265
TTGCGGA251.3478711E-9252.127266
CCGACCG251.3478711E-9252.127268
AACCTAG100.0013523949252.127262
GTCTTGA100.0013523949252.127263
ATTGCGG100.0013523949252.127265
Produced by FastQC (version 0.11.5)