FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61904
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG890514.385177048332903No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG38616.237076764021711No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG33805.460067200827087No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG32735.287218919617472No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG25424.106358232101318No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG21293.4391961747221504No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG7111.1485525975704316No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG6991.129167743602998No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTACTTAATGCGTTAGCTACG6341.0241664512794004No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG4750.7673171362109072No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTCATAGGGTTACCTGCG4710.7608555182217628No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGAAGCTTAATGCGTTAGCTGCG4540.7333936417678987No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTGCG3320.536314293098992No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG3310.5346988886017059No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG2870.46362109072111657No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG2780.4490824502455415No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG2750.44423623675368307No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATTGCTTAATGCGTTAGCTGCG2550.4119281468079607No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG2130.34408115792194366No Hit
AACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG1970.31823468596536575No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTACG1930.3117730679762213No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1860.3004652364952184No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG1700.2746187645386405No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGATAACTTAATGCGTTAGCTGCG1690.2730033600413544No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1640.26492633755492373No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTCGCTGCG1490.24069527009563194No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTACG1430.2310028431119152No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG1430.2310028431119152No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG1330.21484879813905403No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1300.21000258464719565No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1280.20677177565262342No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTGCG1260.2035409666580512No Hit
CACTNTTGNGAGNGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG1250.20192556216076504No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG1220.1970793486689067No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1220.1970793486689067No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTACTGCGTTAGCTGCG1160.18738692168518997No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGCG1120.18092530369604548No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG1010.16315585422589815No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG980.1583096407340398No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATTACTTAATGCGTTAGCTGCG940.1518480227448953No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTACG920.14861721375032308No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTAATGCGTTAGCTGCG890.1437710002584647No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCG880.1421555957611786No Hit
TAATCTTGCGACCGTACTCCCCATGCGGATAACTTAATGCGTTAGCTGCG870.14054019126389247No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCT870.14054019126389247No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTACG850.13730938226932024No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTACG840.13569397777203412No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATATCTTAATGCGTTAGCTGCG820.13246316877746186No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGCTTACTTATCACTTTCGCTTGG810.13084776428017575No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTTACTTATCACTTTCGCTTGG750.12115533729645904No Hit
CAGCCTTGCGACCATACTCCCCCCAGAACCCAAAGACTTTGATTTCTCAT740.11953993279917291No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG740.11953993279917291No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTCATGCGTTAGCTGCG730.1179245283018868No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTGCG720.11630912380460068No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG690.1114629103127423No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG680.10984750581545619No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTTAACTTAATGCGTTAGCTGCG670.10823210131817007No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCATCTTCACGCGTTAGCTACG670.10823210131817007No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG660.10661669682088394No Hit
TACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG660.10661669682088394No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG640.10338588782631171No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG640.10338588782631171No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG640.10338588782631171No Hit
TAATCTTGCGACCGTACTCCCCAGGTGGATAACTTAATGCGTTAGCTGCG640.10338588782631171No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG620.10015507883173946No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAG100.0013512418252.14748
AAACTTG100.0013512418252.14742
GCGAACG201.3425415E-7252.14748
CGGCCGC100.0013512418252.14749
CCGGCCG201.3425415E-7252.14748
CGTGAGA100.0013512418252.14745
AGCCATG100.0013512418252.14742
TCGGCCG100.0013512418252.14748
TCGTGAG100.0013512418252.14744
AGCATTG100.0013512418252.14742
TTGCGGT100.0013512418252.14746
ACCGTGC100.0013512418252.14743
CGGTCGT100.0013512418252.14749
AATAGTG100.0013512418252.14742
GTGCGAG100.0013512418252.14746
TGAGAGC100.0013512418252.14747
ATATTGC400.0252.14743
ACCCTTG450.0252.147382
TGCGAGC4850.0252.147387
TCTTGAG301.2732926E-11252.147374
Produced by FastQC (version 0.11.5)