FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83182
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG841310.113966964006636No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG41915.0383496429516No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG37494.506984684186483No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG27593.3168233512057896No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG20092.4151859777355678No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG17902.151907864682263No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG17372.088192156957034No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAACGCGTTAGCTACG17322.0821812411338994No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG14031.6866629799716284No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG11841.4233848669183238No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG8981.0795604818350124No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTAG8661.0410906205669497No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA7570.9100526556226106No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG7480.899233007140968No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG6550.7874299728306605No Hit
AACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG5900.7092880671299079No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG4540.545791156740641No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA4310.5181409439542208No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG3800.45682960255824573No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG3760.45202086989973794No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG3680.44240340458272226No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG3290.39551826116227073No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTGG3230.38830516217450894No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG3220.38710297900988194No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3220.38710297900988194No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3160.37988988002212015No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG2970.3570483998942079No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2880.3462287514125652No Hit
CACANTTGNGTGNGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG2430.2921305090043519No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAACGCGTTAGCTGCG2360.28371522685196315No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA2180.26207592988867784No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG2010.2416388160900195No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1990.23923444976076555No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG1960.23562790026688465No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG1950.23442571710225768No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1930.2320213507730038No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG1910.22961698444374984No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG1890.22721261811449595No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA1870.224808251785242No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG1830.21999951912673416No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG1770.21278642013897237No Hit
TACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1670.20076458849270276No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1650.1983602221634488No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1620.19475367266956792No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1510.18152965785867137No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG1390.1671034598831478No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG1340.16109254406001297No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATACTTAACGCTTTCGCTAAG1310.1574859945661321No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTCG1310.1574859945661321No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG1290.15508162823687818No Hit
CAAACTTGCGTTCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1290.15508162823687818No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGCTTACTTATCACTTTCGCTTGG1280.1538794450722512No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTGCG1200.14426197975523553No Hit
TAATCTTGCGATCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG1190.14305979659060855No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTCTCGCGTTAGCTACG1170.14065543026135463No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1090.13103796494433892No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAACGCTTTCGCTTGG1090.13103796494433892No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGCTACTTATCACTTTCGCTTAG1070.128633598615085No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG1040.1250270491212041No Hit
TAGTNTTGNGACNGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1040.1250270491212041No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG1030.12382486595657716No Hit
AACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG1020.12262268279195018No Hit
AATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1020.12262268279195018No Hit
TAGTCTTGCGACCGTACTCCCCAGTCGGTCTACTTATCGCGTTAGCTGCG990.11901613329806929No Hit
CACACTTGCGAGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG990.11901613329806929No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG950.11420740063956143No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG940.11300521747493449No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG920.11060085114568056No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTTACTTATCACTTTCGCTTGG920.11060085114568056No Hit
CATTCTTGCGAACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG890.10699430165179967No Hit
CAATCTTGCGACCGTACTCCCCAGGTGGCATACTTAATGCTTTCGCTTAG890.10699430165179967No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG880.10579211848717271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT3350.0251.59999
CGGCCGG350.0251.599889
AACATTG151.3617824E-5251.599882
TGAGACC151.3617824E-5251.599887
AGTCGTG301.4551915E-11251.599882
CGGACGT151.3617824E-5251.599889
CGACCGG950.0251.599879
AGTGCGG100.0013609352251.599875
AGTGCGA100.0013609352251.599875
AGACCGT201.3600766E-7251.599879
TTGCCGG5450.0251.599876
GTGCGGT100.0013609352251.599876
GTGCGGG100.0013609352251.599876
GAGGCCG100.0013609352251.599878
AACCTTG18550.0250.921722
TCTTGCG34750.0250.875844
GCGACCG27000.0250.668018
CTTGCGA38250.0249.955435
TGCGGCC13300.0249.708167
GCGGCCG13300.0249.708168
Produced by FastQC (version 0.11.5)