FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78027
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG75759.708177938405935No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG50636.488779525036205No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG17422.2325605239212067No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG16632.1313135196790856No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG14891.9083137888166914No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG14091.8057851769259357No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG12041.5430556089558742No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG11991.5366475707127019No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA10371.3290271316339217No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG10071.2905789021748881No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG9911.270073179796737No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG9151.172670998500519No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG9011.1547284914196367No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG8971.149602060825099No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG8341.0688607789611289No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG8141.04322862598844No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG7660.9817114588539865No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA7350.9419816217463186No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG7330.9394184064490496No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG6710.859958732233714No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG6030.7728094121265716No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAGTGCGTTAGCTGCG5290.6779704461276225No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG4950.6343957860740512No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG4280.5485280736155433No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG4060.5203327053455855No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG4020.5152062747510477No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG3970.5087982365078755No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG3910.5011085906160688No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3790.4857292988324554No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG3710.4754764376433799No Hit
AACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG3070.39345354813077527No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG3020.387045509887603No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAACGCGTTAGCTACG2850.36525817986081743No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTCTCGCGTTAGCTACG2810.3601317492662796No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTAG2780.3562869263203763No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG2710.34731567277993514No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTGG2340.29989618978046056No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAATTTCACGCGTTAGCTACG2210.28323529034821276No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2190.2806720750509439No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2110.2704192138618683No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTGCG2090.2678559985645994No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAATGCGTTAGCTGCG1990.25503992207825493No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG1740.22299973086239377No Hit
CACANTTGNGTGNGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1670.21402847732195268No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1480.18967793199789815No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAACGCTTTCGCTTGG1470.1883963243492637No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1450.18583310905199482No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG1440.18455150140336038No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA1250.16020095607930587No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG1180.15122970253886475No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG1160.14866648724159587No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1160.14866648724159587No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAATTTATTGCGTTAGCTGCG1090.13969523370115472No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAATGCGTTAGCTGCG1070.13713201840388584No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATACTTAACGCTTTCGCTAAG1060.1358504107552514No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1060.1358504107552514No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG1000.12816076486344471No Hit
CAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG990.12687915721481025No Hit
CAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG990.12687915721481025No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG970.12431594191754135No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTTCG950.12175272662027248No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG940.12047111897163802No Hit
CAGCCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCA920.11790790367436912No Hit
TACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG890.11406308072846578No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCTATTTAACGCGTTAGCTCCG850.108936650133928No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG820.10509182718802465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAC450.0251.891116
AACAATG100.001356061251.89112
TCTTGAG201.351691E-7251.89114
AGTGCGA100.001356061251.89115
AGTGAGA100.001356061251.89115
AATTGCG100.001356061251.89114
TTGCGGT1300.0251.89116
GTGAGAC100.001356061251.89116
GTGCGGC201.351691E-7251.89116
GTGCGAG100.001356061251.89116
AGAGCGG100.001356061251.89115
AGACCGT151.355174E-5251.891089
GTGAGGC151.355174E-5251.891086
ATGCGAG151.355174E-5251.891086
ATTGCGA350.0251.891085
CGCCCGT301.4551915E-11251.891089
AGTCGTG151.355174E-5251.891082
AACATTG251.3551471E-9251.891072
ATTGCGG251.3551471E-9251.891075
CGATCGT1700.0251.891079
Produced by FastQC (version 0.11.5)