FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55809
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG797814.295185364367754No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG34426.167464029099249No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG26974.832553889157663No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG26444.737587127524234No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG11852.123313444068161No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG10561.892167929903779No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG7381.3223673601032093No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG7151.2811553692056836No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG6991.2524861581465354No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG6601.1826049561898617No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG5891.0553853321148918No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG5871.0518016807324984No Hit
TAATCTTGCGATCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG4880.8744109373040191No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG4550.815280689494526No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG4330.7758605242881972No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATAACTTAACGCTTTCGCTGTG3910.7006038452579333No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG3630.650432725904424No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3220.5769678725653569No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG2820.5052948449174864No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG2660.4766256338583383No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2660.4766256338583383No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2650.4748338081671415No Hit
CATTCTTGCGAACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG2390.4282463401960257No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTAG2390.4282463401960257No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG2350.42107903743123865No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2230.3995771291368776No Hit
AACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG2040.3655324410041391No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1800.32252862441541685No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG1620.29027576197387517No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG1470.26339837660592375No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG1440.2580228995323335No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG1400.25085559676754643No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA1310.23472916554677561No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1280.22935368847318535No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG1220.21860273432600477No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1080.1935171746492501No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG1060.1899335232668566No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG1040.1863498718844631No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1000.17918256911967603No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTTCG980.1755989177372825No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTGG960.172015266354889No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG940.16843161497249548No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTACTTAATGCGTTAGCTACG920.16484796359010195No Hit
CACANTTGNGTGNGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG920.16484796359010195No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG910.1630561378989052No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTTGG900.16126431220770843No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG890.15947248651651166No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG890.15947248651651166No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG850.15230518375172464No Hit
CAATCTTGCGACCGTACTCCCCAGGTGGCATACTTAATGCTTTCGCTTAG850.15230518375172464No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG850.15230518375172464No Hit
CAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG830.1487215323693311No Hit
TAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG810.14513788098693758No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG800.14334605529574082No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA780.1397624039133473No Hit
CAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG780.1397624039133473No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG770.13797057822215053No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTGCG770.13797057822215053No Hit
CAACCTTGCGGCCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTTCG740.13259510114856027No Hit
CAAACTTGCGTTCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG740.13259510114856027No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGCTTACTTATCACTTTCGCTTGG740.13259510114856027No Hit
CAATCTTGCGATCGTACTCCCCAGGTGGATCACTTAACGCTTTCGCTTGG740.13259510114856027No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG720.12901144976616674No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTTAG710.12721962407496998No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCTCGCGTTAGCTTCG700.12542779838377321No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATGACTTAACGATTTCTCTGTA700.12542779838377321No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTCTCGCGTTAGCTACG660.11826049561898619No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTGG660.11826049561898619No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG610.10930136716300239No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG590.10571771578060886No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG590.10571771578060886No Hit
TACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG580.1039258900894121No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG580.1039258900894121No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTACG570.10213406439821533No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGATAACTTAATGCGTTAGCTGCG570.10213406439821533No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTTTG560.10034223870701857No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG560.10034223870701857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGCG251.3442332E-9252.069264
CAACCTT12900.0252.069261
GCCGGCG3250.0252.069268
GCCGCCG251.3442332E-9252.069268
ATCTTGC15800.0252.069263
TTGCGGA251.3442332E-9252.069266
ATTGCGG251.3442332E-9252.069265
AAGTCTT251.3442332E-9252.069261
CAGCCTT301.2732926E-11252.069241
CGTGCGA301.2732926E-11252.069245
CGGCCGT15150.0252.069249
CGGCCGC151.3492321E-5252.069249
ACATTGC151.3492321E-5252.069243
CGACCGC151.3492321E-5252.069249
TGCGATC2950.0252.069247
TGCGAGC1250.0252.069247
TGCGAAC3400.0252.069247
AGTCTTG2150.0252.069242
GTCTTGC2150.0252.069243
CGATCGT2900.0252.069249
Produced by FastQC (version 0.11.5)