FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75680
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG845611.17336152219873No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG32324.2706131078224105No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG27203.5940803382663846No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG27193.592758985200846No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG25763.4038054968287526No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG25653.3892706131078225No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG23073.0483615221987317No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG19472.572674418604651No Hit
TAATCTTGCGATCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG9461.25No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG8211.084830866807611No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG7651.0108350951374208No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG7300.9645877378435518No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG6980.9223044397463002No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG6480.8562367864693446No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG5970.7888477801268499No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG5770.7624207188160677No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATGACTTAACGATTTCTCTGTA5680.7505285412262156No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG5250.6937103594080338No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3140.41490486257928116No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3090.4082980972515856No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATAACTTAACGCTTTCGCTGTG2810.3713002114164905No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG2760.36469344608879495No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTTGG2570.3395877378435518No Hit
CATTCTTGCGAACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG2520.33298097251585623No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTTAG2520.33298097251585623No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA2210.29201902748414377No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG2170.2867336152219873No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG2160.2854122621564482No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTCTCGCGTTAGCTACG2040.26955602536997886No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2000.2642706131078224No Hit
CAATCTTGCGATCGTACTTCCCAGGTGGATTACTTAACGCTTTCGCTTAG1990.2629492600422833No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG1970.2603065539112051No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1920.2536997885835095No Hit
CAGGCTTGCGCCCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1800.23784355179704017No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA1780.23520084566596194No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG1740.2299154334038055No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG1720.22727272727272727No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG1690.22330866807610994No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1640.2167019027484144No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCTCGCGTTAGCTTCG1620.21405919661733616No Hit
AACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1440.19027484143763213No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG1390.18366807610993657No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTAG1390.18366807610993657No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG1390.18366807610993657No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG1360.17970401691331925No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1330.1757399577167019No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1270.16781183932346724No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTGCG1240.1638477801268499No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1200.15856236786469344No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG1150.15195560253699789No Hit
CAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1130.14931289640591966No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1110.14667019027484143No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTTCG1090.1440274841437632No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG1070.14138477801268498No Hit
TAATCTTGCGATCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCG1050.13874207188160678No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG1020.13477801268498943No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCTACTTCACGCGTTAGCTGCG1000.1321353065539112No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG980.12949260042283298No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTATCGGTTTCCCTTGG920.12156448202959831No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAACGCTTTAGCTTGG920.12156448202959831No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTAG920.12156448202959831No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG910.1202431289640592No Hit
CAATCTTGCGACCGTACTCCCCAGGTGGCATACTTAATGCTTTCGCTTAG870.11495771670190275No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG850.11231501057082452No Hit
TAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG850.11231501057082452No Hit
CAAACTTGCGTTCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG850.11231501057082452No Hit
CACANTTGNGTGNGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG850.11231501057082452No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGATAACTTAATGCGTTAGCTGCG840.1109936575052854No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG840.1109936575052854No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG830.10967230443974631No Hit
CAACCTTGCGGCCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTTCG820.1083509513742072No Hit
CAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG820.1083509513742072No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG810.10702959830866808No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATAACTTAACGATTTCTCTGTA800.10570824524312897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTTG400.0252.111722
CAAACTT201.3455247E-7252.111721
GGAGACC100.0013524255252.111727
CGGCCCG100.0013524255252.111729
TGCGGCC17450.0252.111727
AAAACTT151.3502769E-5252.111721
TTGAGAC201.3455247E-7252.111726
AACATTG100.0013524255252.111722
AGTGAGA100.0013524255252.111725
TGCGAGC2350.0252.111727
AGACCGG151.3502769E-5252.111729
AGTCTTG6850.0252.111722
CTTGAGA151.3502769E-5252.111725
ATCTTGA100.0013524255252.111723
TTGCGGA350.0252.111726
GTGAGAC151.3502769E-5252.111726
TTGCCGT100.0013524255252.111726
GTCTTGA100.0013524255252.111723
ATTGCGA350.0252.111725
GTGCGAG100.0013524255252.111726
Produced by FastQC (version 0.11.5)