FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118342
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG2459220.780449882543813No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG99578.413749978874787No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG83387.04568116137973No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG47504.013790539284447No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG20721.750857683662605No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGCACACTTAACGCGTTAGCTCCG16731.4136992783627114No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGCTCACTTACCGGTTTCCCTACG7660.6472765374930287No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG7650.6464315289584425No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG7600.6422064862855115No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG7020.593195991279512No Hit
CAGCCTTGCGACCATACTCCCCCCAGAACCCAAAGACTTTGATTTCTCAT6420.5424954792043399No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGTTTAATGCGTTAGCTGCG5720.483344881783306No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG5040.42588430143144446No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTCATAGGGTTACCTGCG5040.42588430143144446No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGATAACTTAATGCGTTAGCTGCG5000.42250426729309964No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGCGCTTATCGCGTTGGCTACG4630.39123895151341026No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGAAGCTTAATGCGTTAGCTGCG4580.3870139088404793No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT4540.3836338747021345No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG4100.34645349918034174No Hit
TAATCTTGCGATCGTACTTCCCAGGCGGCACGCTTAACGCGTTAGCTCCG3900.32955332848861774No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG3860.3261732943502729No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG3300.2788528164134458No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTACTTAATGCGTTAGCTACG3300.2788528164134458No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG3180.2687127139984114No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3060.258572611583377No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3020.2551925774450322No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2990.2526575518412736No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG2950.2492775177029288No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTCGCTGCG2920.24674249209917018No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTACTGCGTTAGCTGCG2700.2281523043382738No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGCGCTTACCGCGTTGGCTACG2690.2273072958036876No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG2590.21885721045782563No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGGGCACTTCACGGTTTCCCTTCG2520.21294215071572223No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2470.20871710804279123No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTGCG2390.20195703976610166No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTTAG2260.19097192881648103No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG2220.18759189467813625No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2210.18674688614355006No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCG2150.18167683493603284No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG2110.17829680079768806No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCT2100.17745179226310187No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATATCTTAATGCGTTAGCTGCG2080.17576177519392946No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG2030.17153673252099846No Hit
TAATCTTGCGACCGTACTCCCCATGCGGATAACTTAATGCGTTAGCTGCG1970.16646668131348127No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGCACGCTTAACGCGTTAGCTCCG1870.15801659596761927No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGGGCACTTCACGGTTTCCCTGCG1820.15379155329468827No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTACG1810.15294654476010208No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTAACGCGTTAGCTGCG1700.1436514508796539No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTATCGCGTTTGCTGCG1670.14111642527589527No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG1660.14027141674130908No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGAGCTTAATGCGTTAGCTGCG1640.1385813996721367No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTATCGCGTTAGCTTCG1620.1368913826029643No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTGCG1610.1360463740683781No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGCG1570.1326663399300333No Hit
TAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1540.1301313143262747No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTTCG1530.1292863057916885No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTTAATGCGTTAGCTGCG1510.1275962887225161No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG1500.1267512801879299No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1480.1250612631187575No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1480.1250612631187575No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTAATGCGTTAGCTGCG1470.12421625458417129No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTGCG1470.12421625458417129No Hit
TAATCTTGCGACCGTACTCCCCAGGTGGATAACTTAATGCGTTAGCTGCG1400.11830119484206791No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1390.11745618630748171No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTACCGCGTTAGCTACG1390.11745618630748171No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG1380.11661117777289551No Hit
TAATCTTGCGACCGTACTCCCCCGGCGGATAACTTAATGCGTTAGCTGCG1380.11661117777289551No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGAGCACTTAACACTTTCGCTACG1360.1149211607037231No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG1330.11238613509996451No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1320.1115411265653783No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG1320.1115411265653783No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTGCG1320.1115411265653783No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTGCG1300.10985110949620593No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCATCTTCACGCGTTAGCTGCG1270.10731608389244732No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTCATGCGTTAGCTGCG1270.10731608389244732No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTTCG1270.10731608389244732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGA251.3533281E-9252.073185
TTGCGGT500.0252.073186
AAATATT201.3498357E-7252.073171
CGACCGG1050.0252.073179
AGACCGG201.3498357E-7252.073179
TTGCGGA450.0252.073176
CCGACCG400.0252.073178
TTGCCGG201.3498357E-7252.073176
AAACTTG151.3530842E-5252.073152
AAATCTT2750.0252.073151
TCTTGAG301.4551915E-11252.073154
ATGAGAC151.3530842E-5252.073156
GCGACCG73350.0251.901348
CGACCGT72500.0251.72559
TTGCGAC75350.0251.236836
TGCGACC77050.0250.928127
TGCGGCC23500.0250.46427
AACCTTG21800.0250.338712
ATCTTGC67100.0250.007023
AATCTTG67250.0249.82422
Produced by FastQC (version 0.11.5)