FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39767
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG39029.812155807579149No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG33648.459275278497246No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG13573.412377096587623No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG10712.6931878190459426No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG10682.6856438755752254No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG7131.7929438982070562No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG6901.735106998264893No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG6871.7275630547941763No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG5791.4559810898483667No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG5351.3453365856111852No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG4941.2422360248447204No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA4151.043578846782508No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG4061.0209470163703573No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG3960.9958005381346342No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA3650.9178464556038928No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG3500.8801267382503081No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG3450.8675534991324465No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3180.7996580078959943No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG2950.741821107953831No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG2730.6864988558352403No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG2710.6814695601880957No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2630.6613523775995173No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG2030.5104735081851787No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTGG1930.4853270299494556No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1900.4777830864787387No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAATTTATTGCGTTAGCTGCG1650.41491689088943096No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1640.41240224306585865No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAATTTCACGCGTTAGCTACG1460.36713858224155704No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1290.32438956924082785No Hit
TAGTNTTGNGACNGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1240.3118163301229663No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG1230.309301682299394No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCTATTTAACGCGTTAGCTCCG1140.2866698518872432No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1100.27661126059295393No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1020.25649407800437546No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTGCG980.24643548671008628No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTCTCGCGTTAGCTACG950.23889154323936934No Hit
TAGTCTTGCGACCGTACTCCCCAGTCGGTCTACTTATCGCGTTAGCTGCG940.236376895415797No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG920.23134759976865238No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG860.21625971282721854No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG860.21625971282721854No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATACTTAACGCTTTCGCTAAG810.203686473709357No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG780.1961425302386401No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG760.19111323459149546No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG760.19111323459149546No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAACGCTTTCGCTTGG720.18105464329720622No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG680.17099605200291698No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG670.1684814041793447No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG650.16345210853220007No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG650.16345210853220007No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG630.15842281288505544No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAGTGCGTTAGCTGCG620.15590816506148314No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAATGCGTTAGCTGCG570.1433349259436216No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAATGCGTTAGCTGCG550.13830563029647697No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG540.1357909824729047No Hit
AACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG540.1357909824729047No Hit
TAACNTTGNGGCNGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG530.13327633464933236No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGCTCCG520.13076168682576006No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG490.12321774335504314No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA480.12070309553147082No Hit
TAGCCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG430.10812985641360927No Hit
CAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG420.10561520859003695No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGATACTTATTGCGTTAACTCCG420.10561520859003695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCG12250.0251.911938
CGACCGG201.3426143E-7251.911939
CGACCGC100.001353155251.911939
GCCGGCG2550.0251.911938
ACCCTTG100.001353155251.911932
TCGTGCG100.001353155251.911934
TGCGAGC1000.0251.911937
TGCGAAC1050.0251.911937
TTGCGGG100.001353155251.911936
TTGCGAA1000.0251.911936
CCGACCG100.001353155251.911938
AACCTAG100.001353155251.911932
ATGCGAC201.3426143E-7251.911936
TTGCCGG2550.0251.911936
GTCTTGC5500.0251.911933
AGAGGCC100.001353155251.911937
CCTGCGA100.001353155251.911935
GTGCGGC201.3426143E-7251.911936
TGAGACC100.001353155251.911937
TGCCGGC2550.0251.911937
Produced by FastQC (version 0.11.5)