FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65136
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG50667.777573077867846No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG37155.703451240481454No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG31034.763878653893392No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG27334.195836403831983No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG26964.1390321788258415No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG12221.8760746745271433No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG11211.7210144927536233No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG8781.3479489069024808No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG8761.3448784082535004No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG7911.2143822156718251No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG7481.1483664947187424No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG7361.1299435028248588No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG7161.0992385163350529No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG7141.0961680176860722No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG5290.8121468926553672No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG5060.7768361581920904No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG4560.7000736919675755No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA4380.6724392041267502No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA4350.6678334561532793No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG4340.6662982068287889No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG4210.6463399656104151No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG3850.5910709899287645No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTGG3720.5711127487103905No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG3680.5649717514124294No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3580.5496192581675264No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG3220.49435028248587576No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAATGCGTTAGCTGCG2910.4467575534266765No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2840.4360108081552444No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTTCG2260.3469663473348072No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG1990.3055146155735692No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1960.3009088676000983No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAATTTATTGCGTTAGCTGCG1820.27941537705723407No Hit
CAGCCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCA1770.2717391304347826No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCTATTTAACGCGTTAGCTCCG1760.2702038811102923No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1680.2579218865143699No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTGCG1620.24871039056742814No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAACGCTTTCGCTTGG1600.24563989191844757No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1560.23949889462048637No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1550.2379636452959961No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1500.23028739867354459No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG1350.20725865880619013No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATACTTAACGCTTTCGCTAAG1310.20111766150822893No Hit
CACCNTTGNCGGNGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG1150.1765536723163842No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1150.1765536723163842No Hit
TAGTNTTGNGACNGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1130.1734831736674036No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG1040.15966592974699093No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1040.15966592974699093No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGCG1020.15659543109801033No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAATTTCACGCGTTAGCTACG990.15198968312453942No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG950.14584868582657823No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG950.14584868582657823No Hit
TAACNTTGNGGCNGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG880.13510194055514615No Hit
CACCNTTGNCGGNGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG860.13203144190616556No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG790.12128469663473349No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG770.11821419798575289No Hit
TAACCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG740.113608450012282No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG740.113608450012282No Hit
TAGTCTTGCGACCGTACTCCCCAGTCGGTCTACTTATCGCGTTAGCTGCG730.1120732006877917No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG720.1105379513633014No Hit
TAGTCTTGCGACCGTACTCCCCATGCGGTCTACTTATCGCGTTAGCTGCG720.1105379513633014No Hit
CAACCTTGCGGCCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTTCG700.1074674527143208No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTTCG680.1043969540653402No Hit
CAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG680.1043969540653402No Hit
TAACCTTGCGGCCGTACTCCCCAGTCGGTCGATTTAACGCGTTAGCTCCG680.1043969540653402No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG680.1043969540653402No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG670.10286170474084991No Hit
AACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG670.10286170474084991No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAGTGCGTTAGCTGCG660.10132645541635961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGAG100.0013450696252.543234
CGTGCCG100.0013450696252.543235
TTGTCGG100.0013450696252.543236
GCCGGCG10550.0252.543238
AAAACTT100.0013450696252.543231
TTGAGAC100.0013450696252.543236
AGGCCGG100.0013450696252.543239
AACATTG201.3326098E-7252.543232
AACGATG100.0013450696252.543232
TTGCGGA100.0013450696252.543236
TTGCGAT400.0252.543236
GATGCAG100.0013450696252.543235
TTGCCGG10350.0252.543236
CGATGCA100.0013450696252.543234
GCGTGCG1500.0252.543238
GTCTTGA100.0013450696252.543233
TCGACCG100.0013450696252.543238
TGTCGGC100.0013450696252.543237
GTCGGCG100.0013450696252.543238
CGGACGT100.0013450696252.543239
Produced by FastQC (version 0.11.5)