FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80864
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG1686020.849821923229126No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG953811.795112781954888No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG20952.5907696873763353No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG20362.517807677087456No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG10281.2712702809655718No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG9531.1785219628017412No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG9461.1698654531064505No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG8701.0758804907004353No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG8661.0709339137316978No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG8401.0387811634349031No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG7110.8792540561931144No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG6960.8607043925603483No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG6910.8545211713494263No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG5820.7197269489513257No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG5590.6912841313810844No Hit
TAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG5480.6776810447170558No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG5300.6554214483577365No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG5280.6529481598733676No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG5240.64800158290463No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG4690.5799861495844876No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTTCG4490.5552532647407993No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTCGCTTCG3840.4748713889988128No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG3790.4686881677878908No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAATGCGTTAGCTGCG3770.46621487930352196No Hit
TAACCTTGCGGCCGTACTCCCCAGTCGGTCAACTTATCGCGTTAGCTTCG3600.445191927186387No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG3280.40561931143648594No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG3030.37470320538187574No Hit
TAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG2980.36851998417095366No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2920.36110011871784725No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG2870.3549168975069252No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA2500.3091610605461021No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG2360.2918480411555204No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2340.28937475267115154No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2170.26835180055401664No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAGTGCGTTAGCTGCG1960.24238227146814403No Hit
TAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG1830.2263058963197467No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCATCTTATCGCGTTAGCTTCG1820.22506925207756234No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA1820.22506925207756234No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG1720.21270280965571825No Hit
TAACCTTGCGGCCGTACTCCCCATGCGGTCAACTTATCGCGTTAGCTTCG1630.20157301147605858No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1590.19662643450732092No Hit
CAACGTTGCCGTCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG1490.18425999208547683No Hit
CAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1450.17931341511673923No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAATGCGTTAGCTGCG1420.175603482390186No Hit
CATACTTGCGTACGTACTACTCAGGCGGAGTACTTAATATGTTAACTACA1340.16571032845271072No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG1320.1632370399683419No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAACGCGTTAGCTTCG1300.1607637514839731No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTTCG1280.15829046299960428No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATTGCGTTAGCTTCG1270.15705381875741986No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1230.15210724178868223No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG1220.15087059754649781No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1210.14963395330431342No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTTCG1080.1335575781559161No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG1080.1335575781559161No Hit
CAACCTTGCGGCCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTTCG1070.1323209339137317No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG1070.1323209339137317No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCTTTAGCTTCG1050.1298476454293629No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG980.12119113573407202No Hit
CAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG950.1174812030075188No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTAG930.11500791452314998No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG910.11253462603878117No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG910.11253462603878117No Hit
TAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG810.10016818361693709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGAG100.0013528309252.097524
CATGCGA151.3509343E-5252.097525
CGGCCGG850.0252.097529
TTGAGAC100.0013528309252.097526
GAGACCG100.0013528309252.097528
GTAGAGC100.0013528309252.097523
TGCGATC650.0252.097527
CTTGAGA100.0013528309252.097525
GTCGTGC100.0013528309252.097523
TTGCGGA201.3464887E-7252.097526
TTGCGAA1700.0252.097526
AGCCTTG750.0252.097522
ACCGTTG1200.0252.097522
GCGTGCG1500.0252.097528
TGAGACC100.0013528309252.097527
TAGTCTT3100.0252.097521
CCTTGAG400.0252.097524
AGAGCGA100.0013528309252.097525
AGTCGTG100.0013528309252.097522
CGGACGT201.3464887E-7252.097529
Produced by FastQC (version 0.11.5)