FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95797
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG74697.796695094835955No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG69927.298767184776141No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA39424.114951407664123No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG37363.8999133584559016No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG35443.699489545601637No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG23262.428050982807395No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG19292.013632994770191No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG18291.909245592241928No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG12461.3006670355021555No Hit
CATACTTGCGTACGTACTACTCAGGCGGAGTACTTAATATGTTAACTACA11791.2307274758082194No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG10791.1263400732799567No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA10591.105462592774304No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG10551.1012870966731736No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA9801.0229965447769762No Hit
CAACGTTGCCGTCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG9731.0156894265999978No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATACTTAATGCGTTTGCTGCG8960.9353111266532355No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG8760.9144336461475829No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG8310.8674593150098646No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG8260.8622399448834515No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA7530.7860371410378196No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG5360.559516477551489No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGCACTTAATGCGTTAACTGCG5090.5313318788688581No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG5060.5282002567930102No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG4860.5073227762873577No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG4190.43738321659342155No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG4060.42381285426474735No Hit
AAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG3540.36953140495005066No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG3400.3549171685960938No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAACGCGTTAGCTACG3150.3288203179640281No Hit
CAACNTTGNGGTNGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG2580.26931949852291825No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG2570.2682756244976356No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2400.25052976606783095No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2390.2494858920425483No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG2150.22443291543576524No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2100.21921354530935205No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG2070.2160819232335042No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG2030.21190642713237368No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATGCTTATTGCGTTAACTGCA1940.20251156090483002No Hit
CAGCCTTGCGGCCGTACTCCCCAGGTGGAGTGCTTAACGCTTTCGCTTGA1920.20042381285426478No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG1910.19937993882898214No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGAATACTTATTGCGTTAGCTGCG1840.1920728206520037No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG1830.1910289466267211No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1720.17954633234861217No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1710.17850245832332956No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1680.17537083624748165No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGGATACTTATTGCGTTCACTGCG1550.16180047391880747No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG1410.14718623756485066No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGATTACTTATCGCTTTCGCTTAG1390.14509848951428542No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATGCTTAACGATTTCTCTGTG1350.1409229934131549No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTATACTTAATGTGTTAACTGCA1330.13883524536258965No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG1320.137791371337307No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG1290.13465974926145913No Hit
CACTCTTGCGAGCATACTCCCCAGGCGGAGTACTTAATGCGTTTGCTTCA1280.1336158752361765No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG1260.13152812718561124No Hit
TAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG1240.12944037913504597No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTACACTTAATGTGTTAACTGCA1230.12839650510976336No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG1210.12630875705919808No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG1160.12108938693278495No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTTCG1140.1190016388822197No Hit
TAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1120.11691389083165443No Hit
CATCGTTGCCGACGTACTCCCCAGGTGGATAACTTAACGCTTTCGCTTAA1110.11587001680637181No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTCG1060.11065064667995865No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATAACTTAATGCTTTCGCTCTG1050.10960677265467603No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG1040.1085628986293934No Hit
TAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG990.10334352850298026No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTTCG980.10229965447769764No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGCGTTAACTGCA960.10021190642713239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCG4500.0251.731878
ATGAGGC151.3595933E-5251.731876
GCGGGCG450.0251.731878
CTTGAGA151.3595933E-5251.731875
TTGCGGA151.3595933E-5251.731876
TTGCGCG151.3595933E-5251.731876
TTGCCGG4500.0251.731876
TTGAGAC201.357621E-7251.731866
AACATTG251.3624231E-9251.731862
CGGGCGG100.0013591285251.731869
TTGCGAA3850.0251.731866
GTGAGGC100.0013591285251.731866
GCGTGCG3350.0251.731868
GTGCGAC550.0251.731866
TGAGACC251.3624231E-9251.731867
AGTCGTG100.0013591285251.731862
ATCTTGC18950.0251.067673
CTTGCGG36100.0251.034555
CGGCCGT26950.0250.797799
CAACCTT23800.0250.674161
Produced by FastQC (version 0.11.5)