FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82071
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG62177.575148347162823No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG59477.246164905996028No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG45805.580533927940442No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG38414.68009406489503No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG38254.660598749862924No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG36154.404722740066528No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG16562.0177651058230066No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG12461.5181976581252818No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG11641.4182841685857368No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG10771.3122783930986586No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA7980.972328837226304No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG7870.958925808141731No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA7840.955270436573211No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA6850.8346431748120531No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATACTTAATGCGTTTGCTGCG5540.6750252829866823No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG4820.5872963653422038No Hit
CAACGTTGCCGTCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG4690.5714564218786173No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG4570.5568349356045376No Hit
AACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG3970.48372750423413874No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGCACTTAATGCGTTAACTGCG3370.41062007286373997No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3220.3923432150211402No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG3180.38746938626311367No Hit
CATACTTGCGTACGTACTACTCAGGCGGAGTACTTAATATGTTAACTACA3030.3691925284205139No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG2930.3570079565254475No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2920.35578949933594084No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA2770.33751264149334115No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG2240.27293441044948885No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG2240.27293441044948885No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG2170.2644052101229423No Hit
AACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG2070.2522206382278758No Hit
CACANTTGNGTGNGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG2030.2473468094698493No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1970.2400360663328094No Hit
AAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1890.23028840881675622No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1880.22906995162724958No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1600.19495315032106347No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTAG1590.19373469313155683No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG1590.19373469313155683No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1520.1852054928050103No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTACG1480.1803316640469837No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG1440.17545783528895711No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTACG1440.17545783528895711No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG1420.1730209209099438No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1360.16571017777290395No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1320.16083634901487737No Hit
CACTNTTGNGAGNGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG1290.15718097744635742No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG1290.15718097744635742No Hit
CAACNTTGNGGTNGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1230.14987023430931753No Hit
TACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1220.1486517771198109No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTACG1100.13403029084573115No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG1050.1279380048981979No Hit
CAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1040.12671954770869126No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1030.12550109051918462No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAACGCGTTAGCTACG1020.12428263332967796No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCATCTTCACGCGTTAGCTACG1020.12428263332967796No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTACG1010.12306417614017132No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG990.12062726176115801No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTTCG980.11940880457165137No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG960.11697189019263807No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG960.11697189019263807No Hit
TAACCTTGCGGCCGTACTCCCCAGTCGGTCAACTTATCGCGTTAGCTTCG950.11575343300313143No Hit
TAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG940.11453497581362479No Hit
CAGCCTTGCGGCCGTACTCCCCAGGTGGAGTGCTTAACGCTTTCGCTTGA920.1120980614346115No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTACG910.11087960424510486No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATGCTTAACGATTTCTCTGTG890.10844268986609155No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATGCTTATTGCGTTAACTGCA880.10722423267658492No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTCG860.10478731829757161No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG860.10478731829757161No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTCGCTTCG850.10356886110806497No Hit
TAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG840.1023504039185583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAA1550.0251.99066
TCTTGAG100.0013545891251.990594
CGTGCGA700.0251.990595
GCCGGCG1800.0251.990598
TGCGAAC1600.0251.990597
AGACCGG100.0013545891251.990599
CTTGAGA100.0013545891251.990595
TTGCGGA201.3494719E-7251.990596
GTGCGAG201.3494719E-7251.990596
TGAGACC201.3494719E-7251.990597
CGCCCGT100.0013545891251.990599
TGCCGGC1800.0251.990597
CGGACGG100.0013545891251.990599
GCGCGCG151.3533012E-5251.990578
TGCGGCC15450.0251.990577
AGTGCGA151.3533012E-5251.990575
TGCGATC2400.0251.990577
GTGCGGC151.3533012E-5251.990576
GTGCGAC600.0251.990576
TGCGTGC11750.0250.918297
Produced by FastQC (version 0.11.5)