FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42166
Sequences flagged as poor quality0
Sequence length251
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG548813.015225537162642No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG41099.744818099890908No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG17484.145520087274107No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG15003.5573684959445995No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG14353.4032158611203336No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG12182.8885832187070153No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG8311.9707821467533082No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG6501.54152634824266No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG4911.1644452876725324No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG4871.1549589716833468No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA4311.0221505478347483No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATACTTAATGCGTTTGCTGCG4120.9770905468861169No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG4120.9770905468861169No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3840.9106863349618176No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG3320.7873642271024047No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG3200.758905279134848No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA2720.6450694872646208No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2600.616610539297064No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2500.5928947493240999No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2350.557321064364654No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA2250.53360527439169No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG2200.521747379405208No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA2160.5122610634160224No Hit
AAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG2110.5004031684295405No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG2110.5004031684295405No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG2040.4838021154484656No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGCACTTAATGCGTTAACTGCG1940.4600863254755016No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1660.39368211355120236No Hit
CACTCTTGCGAGCATACTCCCCAGGCGGAGTACTTAATGCGTTTGCTTCA1660.39368211355120236No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG1540.3652231655836456No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGGATACTTATTGCGTTCACTGCG1500.35573684959446No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG1480.3509936915998672No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG1440.34150737561068156No Hit
CAACGTTGCCGTCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG1400.332021059621496No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTCCG1360.3225347436323104No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1300.308305269648532No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTTCG1220.28933263767016076No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1180.2798463216809752No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1140.2703600056917896No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1140.2703600056917896No Hit
CAGCCTTGCGGCCGTACTCCCCAGGTGGAGTGCTTAACGCTTTCGCTTGA1080.2561305317080112No Hit
CAACNTTGNGGTNGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1080.2561305317080112No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1010.2395294787269364No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG970.2300431627377508No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG970.2300431627377508No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG960.2276715837404544No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAGCGCCG940.22292842574586158No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG890.2110705307593796No Hit
AACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG890.2110705307593796No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG890.2110705307593796No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT840.1992126357728976No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG800.189726319783712No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG800.189726319783712No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTACG700.166010529810748No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG680.1612673718161552No Hit
CATCGTTGCCGACGTACTCCCCAGGTGGATAACTTAACGCTTTCGCTTAA680.1612673718161552No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG670.1588957928188588No Hit
CATACTTGCGTACGTACTACTCAGGCGGAGTACTTAATATGTTAACTACA660.1565242138215624No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTGCG660.1565242138215624No Hit
CAACCTTGCGGCCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTTCG620.1470378978323768No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTAATGTGTTAACGCCG600.14229473983778398No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG600.14229473983778398No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG570.1351800028458948No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGCACACTTAACGCGTTAGCTACG560.1328084238485984No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGCACACTTATCGCGTTTGCTTCG550.130436844851302No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATGCTTAACGATTTCTCTGTG550.130436844851302No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGAATACTTATTGCGTTAGCTGCG540.1280652658540056No Hit
CACCNTTGNCGGNGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG500.11857894986482No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATAACTTAATGCTTTCGCTCTG500.11857894986482No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTAG500.11857894986482No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG490.11620737086752359No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG490.11620737086752359No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCG480.1138357918702272No Hit
CACTNTTGNGAGNGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG470.11146421287293079No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTTCG460.1090926338756344No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTAAG460.1090926338756344No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG460.1090926338756344No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG450.106721054878338No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAGCTGCG450.106721054878338No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG440.1043494758810416No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTACG430.1019778968837452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGC251.3187673E-9252.676483
GAGGCCG301.2732926E-11252.676478
AAACTTG100.0013412131252.676472
AACGTGC100.0013412131252.676473
AACCGTT100.0013412131252.676471
CAGTCTT650.0252.676471
AAATCTT450.0252.676471
ACTTGCG1550.0252.676474
GCGACCA100.0013412131252.676478
TGCGATC1250.0252.676477
GCGGCCT100.0013412131252.676478
TGCGAAC1450.0252.676477
ATGCGAC151.3339286E-5252.676476
CCTTGAG201.3233876E-7252.676474
CCGTTGC2250.0252.676473
TAGTCTT1350.0252.676471
CGGACGT301.2732926E-11252.676479
GCGGACG400.0252.676478
GCGGACC100.0013412131252.676478
AACTCTT400.0252.676471
Produced by FastQC (version 0.11.5)