FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39181
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG726318.537046017202215No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG27376.98552870013527No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG14673.744161711033409No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG13583.4659656466144306No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG12473.1826650672519845No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG6921.7661621704397539No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATACTTAATGCGTTTGCTGCG4161.0617391082412393No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG4111.0489778208825706No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3730.9519920369566882No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG3620.9239172047676169No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA3390.8652152829177407No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA3370.8601107679742733No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG3310.8447972231438708No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG2630.6712437150659759No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGGATACTTATTGCGTTCACTGCG2350.5997805058574309No Hit
TAGTNTTGNGACNGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG2250.5742579311400934No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAATGCGTTAGCTGCG2080.5308695541206196No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG1950.497690206988081No Hit
TAGTCTTGCGACCGTACTCCCCAGTCGGTCTACTTATCGCGTTAGCTGCG1880.47982440468594467No Hit
CACTCTTGCGAGCATACTCCCCAGGCGGAGTACTTAATGCGTTTGCTTCA1700.43388377019473723No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTCCG1540.3930476506469972No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGCACTTAATGCGTTAACTGCG1540.3930476506469972No Hit
AAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1510.385390878231796No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG1500.3828386207600623No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG1460.37262959087312725No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1450.37007733340139354No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG1260.32158444143845233No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG1190.303718639136316No Hit
TAGTCTTGCGACCGTACTCCCCATGCGGTCTACTTATCGCGTTAGCTGCG1190.303718639136316No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1190.303718639136316No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG1180.3011663816645823No Hit
CATACTTGCGTACGTACTACTCAGGCGGAGTACTTAATATGTTAACTACA1130.2884050943059136No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTCGCTGCG1080.2756438069472449No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG1060.27053929200377735No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA900.22970317245603736No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG860.21949414256910238No Hit
TAGCCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG850.21694188509736861No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG780.19907608279523237No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATGCTTAACGATTTCTCTGTG780.19907608279523237No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTAATGTGTTAACGCCG750.19141931038003115No Hit
CAACNTTGNGGTNGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG740.1888670529082974No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG740.1888670529082974No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG740.1888670529082974No Hit
CAGCCTTGCGGCCGTACTCCCCAGGTGGAGTGCTTAACGCTTTCGCTTGA710.18121028049309615No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATTGCGTTAGCTGCG700.1786580230213624No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATAACTTAATGCTTTCGCTCTG700.1786580230213624No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG680.17355350807789488No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCGCTTTCGCTTAG670.17100125060616117No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGATTACTTATCGCTTTCGCTTAG650.16589673566269364No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG650.16589673566269364No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCTTTAGCTGCG640.1633444781909599No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCT620.15823996324749243No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGCTGACTTAACGCGTTAGCTACG620.15823996324749243No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG620.15823996324749243No Hit
TAGTCTTGCGACCGTCCTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG570.14547867588882366No Hit
CAACGTTGCCGTCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG570.14547867588882366No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTCAG570.14547867588882366No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG570.14547867588882366No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTACG540.13782190347362244No Hit
TAGTCGTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG520.1327173885301549No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGCG510.13016513105842117No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG510.13016513105842117No Hit
TAGTCTTGCGACCGTACTCCCCAGGTGGTCTACTTATCGCGTTAGCTGCG500.12761287358668744No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG500.12761287358668744No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG490.12506061611495367No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG480.12250835864321993No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG480.12250835864321993No Hit
TAGTCTTGCGACCGTACTCCCCCGGCGGTCTACTTATCGCGTTAGCTGCG460.11740384369975244No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG450.11485158622801868No Hit
CATCGTTGCCGACGTACTCCCCAGGTGGATAACTTAACGCTTTCGCTTAA440.11229932875628493No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTCTCGCGTTAGCTGCG430.10974707128455119No Hit
TAGTCTTGCGACCGTACTCCCCAGGCTGTCTACTTATCGCGTTAGCTGCG420.10719481381281744No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG410.10464255634108367No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG400.10209029886934995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTG2850.0252.021072
TGCCGGC2750.0252.021067
TTCCGAC151.3470983E-5252.021066
TGCGATC2200.0252.021067
TGCGAAC1200.0252.021067
AGTATTG151.3470983E-5252.021062
GCGTGCG950.0252.021068
ATTGCGG151.3470983E-5252.021065
GCGCCCG151.3470983E-5252.021068
TTGCGGT3550.0252.021066
TTGCGGC3500.0252.021066
AGACTTG151.3470983E-5252.021062
TTGCGAT2200.0252.021066
TTGCGAA1200.0252.021066
ATCGTGC151.3470983E-5252.021063
AGTCCTG151.3470983E-5252.021062
AGACCGT151.3470983E-5252.021069
GTGCGAG151.3470983E-5252.021066
GTGCGAC350.0252.021066
TTGCCGG2750.0252.021066
Produced by FastQC (version 0.11.5)