FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74392
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1490720.038444994085385No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG38925.231745348962254No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG30514.101247445961931No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG26833.606570598989139No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG26793.6011936767394346No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG16922.274438111624906No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTAG13111.7622862673405744No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG12581.6910420475319927No Hit
CAGCCTTGCGACCATACTTCCCCCAGAACCCAAAAACTTTGATTTCTCCT9911.3321324873642328No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG5460.7339498870846327No Hit
CAATCTTGCGACCGTACTCCCCAGGTGGCATACTTAATGCTTTCGCTTAG5080.6828691257124422No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG4600.618346058715991No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG4300.578019141843209No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG3980.5350037638455748No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTGG3870.5202172276588881No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG3670.49333261641036674No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTACG3420.459726852349715No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA3300.4435960856006022No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTACG3120.419399935476933No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG3090.4153672437896548No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2780.37369609635444667No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA2690.3615980212926121No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTACG2660.35756532960533394No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCATCTTCACGCGTTAGCTACG2530.34009033229379504No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG2500.3360576406065168No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2500.3360576406065168No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAACGCGTTAGCTACG2240.3011076459834391No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2200.2957307237337348No Hit
AACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG2010.2701903430476395No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1660.22314227336272716No Hit
TAATCTTGCGACCGTACTCCCCCGGCGGTCAACTTCACGCGTTAGCTACG1500.2016345843639101No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTACG1480.19894612323905794No Hit
TAATCTTGCGACCGTACTCCCCAGGCTGTCAACTTCACGCGTTAGCTACG1270.17071728142811055No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1250.1680288203032584No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCTTTAGCTACG1210.16265189805355415No Hit
AACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG1190.15996343692870202No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG1160.1559307452414238No Hit
CACANTTGNGTGNGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1120.15055382299171954No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG1080.14517690074201528No Hit
TAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1030.13845574792988494No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCACCTTCACGCGTTAGCTACG1000.13442305624260673No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTGCG970.13039036455532851No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG970.13039036455532851No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA960.12904613399290246No Hit
CAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG950.1277019034304764No Hit
TAATCTTGCGACCGTCCTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG930.12501344230562425No Hit
TAATCTTGCGACCGTACTACCCAGGCGGTCAACTTCACGCGTTAGCTACG920.12366921174319818No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG910.12232498118077213No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG890.11963652005591999No Hit
CACTNTTGNGAGNGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG880.11829228949349392No Hit
TAATCGTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG850.11425959780621572No Hit
TAATCTTGCGACCGTACTCCCCAGGCAGTCAACTTCACGCGTTAGCTACG830.11157113668136358No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG820.11022690611893753No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG810.10888267555651146No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG810.10888267555651146No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG800.10753844499408538No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATACTTAATGCGTTTGCTGCG790.10619421443165931No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG780.10484998386923325No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG770.1035057533068072No Hit
TACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG760.10216152274438112No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG760.10216152274438112No Hit
AAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTAG760.10216152274438112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGA550.0252.239965
GCGACTG100.0013503187252.239948
CGTGCGA900.0252.239945
CGACCGG1050.0252.239949
AAGCATG100.0013503187252.239942
AGTGAGA100.0013503187252.239945
AGTCTTG2950.0252.239942
GTCCGAC100.0013503187252.239946
ATGCGAG100.0013503187252.239946
GTGAGAC100.0013503187252.239946
TTACGAC100.0013503187252.239946
AATAGTG100.0013503187252.239942
ATAGTGA100.0013503187252.239943
ATTGCGG100.0013503187252.239945
AAGGCGA100.0013503187252.239945
GTGCGAG100.0013503187252.239946
AAGTCTT201.3419412E-7252.239941
GCCGGCG251.3424142E-9252.239938
TGCGGGC151.3474413E-5252.239937
TGCGGCC8050.0252.239937
Produced by FastQC (version 0.11.5)