FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62051
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG39426.352838793895345No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG36595.896762340655267No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG25194.059563907108669No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG24253.908075615219739No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG21093.3988171020612077No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG12151.9580667515430854No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG11371.8323637008267395No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGCACACTTAACGCGTTAGCTCCG7421.1957905593785756No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG6991.1264927237272566No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG6581.0604180432225105No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG6571.058806465649224No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAGTGCGTTAGCTGCG6411.0330212244766401No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG5780.9314918373595913No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG5740.9250455270664454No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTAG4890.7880614333370937No Hit
CAATCTTGCGATCGTACTTCCCAGGTGGATTACTTAACGCTTTCGCTTAG4860.7832267006172342No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAGAACTTAGCGCGTTAGCTTCG4800.7735572351775153No Hit
TAATCTTGCGATCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG4350.7010362443796232No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG3770.6075647451290068No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAGTGCGTTAGCTGCG3610.581779503956423No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAATTTCACGCGTTAGCTACG3120.502812202865385No Hit
TAGCCTTGCGGCCGTACTTCCCAGGCGGAGTACTTATCGCGTTTGCTTCG2610.42062174662777396No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2610.42062174662777396No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTATCGCGTTAACTTCG2450.3948365054551901No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG2430.3916133503086171No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG2400.38677861758875764No Hit
CAGTCTTGCGACCATACTCCCCCCAGAACCCAAAGACTTTGATTTCTCAT2170.3497123334031684No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTTCG2150.34648917825659536No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG2140.3448776006833089No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG2070.33359655767030344No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCGACG2060.331984980097017No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG1960.31586920436415206No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTACCGCGTTAGCTACG1920.3094228940710061No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGCACGCTTAACGCGTTAGCTCCG1820.2933071183381412No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1790.28847238561828176No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1770.28524923047170875No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTTAG1710.27557976503198983No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG1650.26591029959227086No Hit
TAATCTTGCGATCGTACTACTCAGGTGGATCACTTAACGCGTTAGCTGCG1600.25785241172583845No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG1540.24818294628611948No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTCATAGGGTTACCTGCG1540.24818294628611948No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG1480.23851348084640056No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1380.22239770511353565No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1370.22078612754024915No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTAACGCGTTAGCTGCG1360.21917454996696265No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGCG1320.21272823967381668No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1300.20950508452724373No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATTGCTTAATGCGTTAGCTGCG1200.19338930879437882No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTATCGCGTTAGCTACG1160.18694299850123286No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1130.1821082657813734No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCTACTTCACGCGTTAGCTGCG1110.17888511063480042No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATGCGTTAGCTGCG980.15793460218207603No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTTGG980.15793460218207603No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGAGCACTTAACACTTTCGCTACG980.15793460218207603No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGAAGCTTAATGCGTTAGCTGCG980.15793460218207603No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGTAAACTTAACGCTTTCGCTTGG970.15632302460878955No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGACACTTATCGCGTTAGCTTGG930.14987671431564356No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAATGCGTTAGCTGCG910.1466535591690706No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGCGCTTACCGCGTTGGCTACG900.1450419815957841No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAACGCTTTAGCTTGG900.1450419815957841No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGCGCTTACCGCGTTAGCTACG860.13859567130263814No Hit
TAGTCTTGCGACCGTAGTCCTCAGGCGGCACACTTAACGCGTTAGCTTGG830.13376093858277868No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT800.12892620586291922No Hit
CATTCTTGCGAACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG780.12570305071634624No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGTACTTAACGCGTTAGCTTCG780.12570305071634624No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTGCG770.12409147314305974No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG750.12086831799648676No Hit
CAGTNTTGNGACNGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG750.12086831799648676No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG750.12086831799648676No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGCGAACTTATCGGTTTCCCTTAG740.11925674042320028No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG740.11925674042320028No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCTACTTCACGCGTTAGCTGCG730.11764516284991378No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG730.11764516284991378No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG700.11281043013005432No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG690.11119885255676783No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG680.10958727498348132No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG680.10958727498348132No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG670.10797569741019485No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTACG670.10797569741019485No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGATAACTTAATGCGTTAGCTGCG670.10797569741019485No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA670.10797569741019485No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG660.10636411983690834No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTATCGCTTTCGCTTGG640.10314096469033536No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGAGCTTAATGCGTTAGCTGCG640.10314096469033536No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCTCGCGTTAGCTGCG630.10152938711704888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACG1550.0252.527478
CGACCGT30200.0252.527479
GCCGGCG450.0252.527478
GCGAGCG650.0252.527478
AAACTTG151.340221E-5252.527452
CAAACTT100.0013451646252.527451
GCGACTG151.340221E-5252.527458
GCGACCG30600.0252.527458
GCGACCA750.0252.527458
CGGCCGA151.340221E-5252.527459
ATAGCGA100.0013451646252.527455
AATATTG201.3325007E-7252.527452
AATATAG100.0013451646252.527452
AGTGCGA100.0013451646252.527455
AACATGC100.0013451646252.527453
AGACCGT201.3325007E-7252.527459
CTTGAGA100.0013451646252.527455
TTGCGGT1100.0252.527456
CGAGCGG100.0013451646252.527459
CGAGCGA100.0013451646252.527455
Produced by FastQC (version 0.11.5)