FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84388
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1629519.309617481158458No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG45655.409536900981182No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG40454.793335545338199No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG30053.560932834052235No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG26063.0881167938569467No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG16932.00620941366071No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG10981.3011328624922975No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG10941.2963928520642745No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG7500.8887519552543015No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA6860.8129117884059345No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG5820.689671517277338No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA5730.6790064938142863No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG5390.6387164051760914No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG5210.6173863582499881No Hit
TAGTNTTGNGACNGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG4910.581836280039816No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG4840.5735412617907759No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGCTGACTTAACGCGTTAGCTACG4760.5640612409347301No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG4030.47755605062331136No Hit
TAGTCTTGCGACCGTACTCCCCAGTCGGTCTACTTATCGCGTTAGCTGCG3780.44793098544816795No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG3520.4171209176660189No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG3200.3792008342418353No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA2910.344835758638669No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG2850.3377257429966346No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG2830.3353557377826231No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2780.32943072474759444No Hit
AAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG2620.3104706830355027No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTCGCTGCG2430.2879556335023937No Hit
TAGTCTTGCGACCGTACTCCCCATGCGGTCTACTTATCGCGTTAGCTGCG2380.28203062046736505No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2340.2772906100393421No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG2210.2618855761482675No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1990.23581551879414137No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATACTTAATGCGTTTGCTGCG1980.2346305161871356No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG1970.23344551358012985No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1970.23344551358012985No Hit
CATACTTGCGTACGTACTACTCAGGCGGAGTACTTAATATGTTAACTACA1920.22752050054510117No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG1830.21685547708204955No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1710.20263544579798073No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG1700.201450443190975No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCA1690.2002654405839693No Hit
TAGCCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1650.19552543015594637No Hit
CAACNTTGNGGTNGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1610.1907854197279234No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGGATACTTATTGCGTTCACTGCG1610.1907854197279234No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1580.1872304119069062No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG1560.18486040669289472No Hit
CAACGTTGCCGTCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG1560.18486040669289472No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG1510.17893539365786604No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATAACTTAATGCTTTCGCTCTG1510.17893539365786604No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG1440.1706403754088259No Hit
CAGCCTTGCGGCCGTACTCCCCAGGTGGAGTGCTTAACGCTTTCGCTTGA1430.16945537280182016No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGCACTTAATGCGTTAACTGCG1410.1670853675878087No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG1400.16590036498080296No Hit
TAGTCTTGCGACCGTCCTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1210.14338531544769398No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATTGCGTTAGCTGCG1200.14220031284068826No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1160.1374603024126653No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCTTTAGCTGCG1160.1374603024126653No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTCTCGCGTTAGCTGCG1150.13627529980565958No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1130.1339052945916481No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1120.13272029198464236No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCT1080.12798028155661942No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG1070.12679527894961368No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAATGCGTTAGCTGCG1060.12561027634260796No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATGCTTAACGATTTCTCTGTG1060.12561027634260796No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGCG1050.12442527373560221No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATTGCTTAATGCGTTAGCTGCG1040.12324027112859649No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1020.12087026591458502No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTAATGTGTTAACGCCG1010.11968526330757927No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTACG1000.11850026070057355No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG970.11494525287955634No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG950.11257524766554487No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG940.11139024505853912No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG930.1102052424515334No Hit
TAGTCGTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG910.10783523723752193No Hit
TAGTCTTGCGACCGTACTCCCCCGGCGGTCTACTTATCGCGTTAGCTGCG910.10783523723752193No Hit
CATCGTTGCCGACGTACTCCCCAGGTGGATAACTTAACGCTTTCGCTTAA860.10191022420249325No Hit
TAGTCTTGCGACCGTACTCCCCAGGTGGTCTACTTATCGCGTTAGCTGCG850.1007252215954875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAG100.0013578231251.79442
AAGAGAC100.0013578231251.79446
ACATTGC100.0013578231251.79443
TTGAGAC201.3549652E-7251.79446
AATGCGA201.3549652E-7251.79445
GAGACCG400.0251.79448
AACATGC100.0013578231251.79443
AGACCGG100.0013578231251.79449
GTCGTGA100.0013578231251.79443
TTGCGGA100.0013578231251.79446
ATTGCGG100.0013578231251.79445
CGATCGT3100.0251.79449
ATTGAGA100.0013578231251.79445
TAGTCGT201.3549652E-7251.79441
TCGCGGC100.0013578231251.79446
TACCGTT201.3549652E-7251.79441
TGCCGGC4700.0251.79447
TCTTGAG151.3576568E-5251.794394
TGCGGGC350.0251.794397
AGACCGT301.4551915E-11251.794399
Produced by FastQC (version 0.11.5)