FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101908
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG4707846.19656945480237No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG18071.7731679554107629No Hit
TAGTNTTGNGACNGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG16011.5710248459394747No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG13111.2864544491109628No Hit
TAGTCTTGCGACCGTACTCCCCAGTCGGTCTACTTATCGCGTTAGCTGCG12501.226596538053931No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG9910.9724457353691565No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG8470.8311418141853436No Hit
TAGTCTTGCGACCGTACTCCCCATGCGGTCTACTTATCGCGTTAGCTGCG6990.6859127840797582No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG6730.6603995760882364No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTCGCTGCG6190.6074106056443066No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG4860.47690073399536836No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTAATGTGTTAACGCCG4820.4729756250735958No Hit
TAGCCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG4520.44353730816030146No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG4290.42096793186010906No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA3670.36012874357263414No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3370.3306904266593398No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCTTTAGCTGCG3280.3218589315853515No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCT3260.31989637712446517No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTCTCGCGTTAGCTGCG2960.2904580602111709No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG2910.28555167405895515No Hit
TAGTCTTGCGACCGTCCTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG2860.2806452879067394No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG2840.2786827334458531No Hit
TAGTCGTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG2780.27279507006319426No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATTGCGTTAGCTGCG2740.26886996114142164No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG2420.23746908976724104No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG2350.23060014915413904No Hit
TAGTCTTGCGACCGTACTCCCCAGGTGGTCTACTTATCGCGTTAGCTGCG2330.22863759469325273No Hit
TAGTCTTGCGACCGTACTCCCCCGGCGGTCTACTTATCGCGTTAGCTGCG2270.22274993131059387No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTTCG2210.216862267927935No Hit
TAGTCTTGCGACCGTACTCCCCAGGCTGTCTACTTATCGCGTTAGCTGCG2170.21293715900616242No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1700.16681712917533462No Hit
CAGCCTTGCGACCATACTTCCCCCAGAACCCAAAAACTTTGATTTCTCCT1620.15896691133178945No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1580.15504180241001686No Hit
TAGTCTTGCGACCGTACTACCCAGGCGGTCTACTTATCGCGTTAGCTGCG1540.1511166934882443No Hit
TAGTCATGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1420.13934136672292655No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG1410.1383600894924834No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGCTGCG1400.13737881226204027No Hit
TAGTCTTGCGACCGGACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1360.13345370334026768No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTACG1290.12658476272716568No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTGCG1290.12658476272716568No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGGATGCTTAACGCGTTAGCTAAG1280.12560348549672254No Hit
TAGTCCTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1270.12462220826627939No Hit
TAGTCTTGCGACCGTACTCCTCAGGCGGTCTACTTATCGCGTTAGCTGCG1240.12167837657494995No Hit
TAGTATTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1200.11775326765317738No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCTCGTTAGCTGCG1130.11088432704007536No Hit
TAGTCTTGCGACCGCACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1130.11088432704007536No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG1110.10892177257918907No Hit
TAGTCTTGCGACCGTACTCCCCAGGCAGTCTACTTATCGCGTTAGCTGCG1100.10794049534874593No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG1040.10205283196608707No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAGCTGCG1030.10107155473564391No Hit
AACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG1020.10009027750520078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAGC4500.0252.886377
CTTGAGA450.0252.886375
TTGCCGG900.0252.886376
AGCCGTG100.0013407554252.886352
GAGTGCG100.0013407554252.886354
ACAGTGC100.0013407554252.886353
GAATGCG100.0013407554252.886354
CATGCGA650.0252.886355
CGTGCGA1150.0252.886355
GTCTCGC201.3273893E-7252.886353
TTGAGAC550.0252.886356
AGTGCGG100.0013407554252.886355
GAGACCG800.0252.886358
TCGTGAG100.0013407554252.886354
GTCGTGA100.0013407554252.886353
TTGCGAA550.0252.886356
CGAGCGA100.0013407554252.886355
ATGCGAG100.0013407554252.886356
GTGAGAC100.0013407554252.886356
GTCTTGA400.0252.886353
Produced by FastQC (version 0.11.5)