FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59196
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG59019.968578958037705No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG43067.274140144604366No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG37586.348401919048584No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG20453.454625312521116No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA20173.4073248192445433No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG14752.4917224136765994No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG13402.263666463950267No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG11691.974795594296912No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG8771.4815190215555105No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG8011.3531319683762417No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGGATGCTTAACGCGTTAGCTAAG6321.0676397053854991No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG5880.9933103588080275No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG5540.9358740455436177No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG5360.9054665855801067No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG4200.7095073991485911No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG4110.6943036691668356No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTTCG3660.618285019258058No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG3490.5895668626258531No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG3360.5676059193188728No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG3290.5557807959997297No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3260.5507128860058113No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG3090.5219947293736064No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2870.48483005608487056No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATGCTTATTGCGTTAACTGCA2870.48483005608487056No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG2780.46962632610311505No Hit
CAACGTTGCCGTCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG2460.4155686195013176No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGCACACTTAACGCGTTAGCTCCG2430.41050070950739914No Hit
CAAACTTGCGTTCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG2330.3936076761943375No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2090.3530643962429894No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATACTTAATGCGTTTGCTGCG2070.34968578958037705No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAACGCGTTAGCTACG2040.34461787958645856No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1920.3243462396107845No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA1890.31927832961686603No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGCACTTAATGCGTTAACTGCG1800.3040745996351105No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGCGCTTACCGCGTTGGCTACG1690.28549226299074265No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGAATACTTAATGCGTTAGCTGCG1500.2533954996959254No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGATTACTTATCGCTTTCGCTTAG1480.25001689303331304No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1410.2381917697141699No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG1310.22129873640110817No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG1310.22129873640110817No Hit
TAGTNTTGNGACNGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1280.21623082640718966No Hit
TAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG1260.21285221974457733No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG1170.1976484897628218No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTAATGTGTTAACGCCG1160.19595918643151564No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG1160.19595918643151564No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG1150.19426988310020946No Hit
AACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1120.18920197310629097No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTCGCTTCG1100.18582336644367864No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG1050.17737684978714777No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTTTG1040.17568754645584161No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA980.1655517264680046No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTTCG970.1638624231366984No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG970.1638624231366984No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAATGCGTTAGCTGCG950.16048381647408608No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG920.1554159064801676No Hit
TAACCTTGCGGCCGTACTCCCCAGTCGGTCAACTTATCGCGTTAGCTTCG920.1554159064801676No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG890.15034799648624908No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTACG880.1486586931549429No Hit
CATACTTGCGTACGTACTACTCAGGCGGAGTACTTAATATGTTAACTACA870.14696938982363672No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTTCG840.1419014798297182No Hit
CAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG840.1419014798297182No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG830.14021217649841206No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAACGCGTTAGCTGCG800.13514426650449354No Hit
TAGTCTTGCGACCGTACTCCCCAGTCGGTCTACTTATCGCGTTAGCTGCG790.1334549631731874No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTAAG770.13007635651057503No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG770.13007635651057503No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG760.12838705317926888No Hit
CAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG730.12331914318535037No Hit
CAACCTTGCGGCCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTTCG700.11825123319143184No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTAG670.11318332319751334No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG660.11149401986620719No Hit
CACCNTTGNCGGNGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG650.10980471653490101No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTTGG630.10642610987228866No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG620.1047368065409825No Hit
TAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG620.1047368065409825No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG610.10304750320967633No Hit
AAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG600.10135819987837015No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGCG600.10135819987837015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG251.3442332E-9252.073182
CGTGCGG251.3442332E-9252.073185
CCGTGCG251.3442332E-9252.073184
CCGTCGT251.3442332E-9252.073189
AACATTG251.3442332E-9252.073182
CGGTCGT2350.0252.073189
TCGACCG251.3442332E-9252.073188
TGCGGGC700.0252.073177
GACTTGC201.3439967E-7252.073173
GAGACCG100.0013522779252.073178
TGCGATC450.0252.073177
CTTGCGG23400.0252.073175
CTTGCAG100.0013522779252.073175
AAACCGT100.0013522779252.073171
GCGGGCG650.0252.073178
AGTCTCG100.0013522779252.073172
TTGCGAT450.0252.073176
ATTGCGA100.0013522779252.073175
CGATCGT450.0252.073179
TAGTCTT5850.0252.073171
Produced by FastQC (version 0.11.5)