FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90247
Sequences flagged as poor quality0
Sequence length251
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG76308.45457466730196No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG69897.744301749642648No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG60346.686094828636962No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG59966.643988165811606No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA35993.9879441975910557No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA23342.5862355535364054No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG18792.0820636697064723No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG15761.7463184371779672No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG14851.6454840604119805No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG14591.6166742384788415No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA11431.2665240949837668No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG11161.2366062029762763No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG8110.898644830299068No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG7150.7922701031613238No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG7020.7778651921947544No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTAG6500.7202455483284763No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG6470.7169213381054218No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG5660.62716766208295No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATGCTTATTGCGTTAACTGCA5650.6260595920085986No Hit
CAGCCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCA4680.518576794796503No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG4640.5141445144990969No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG4530.5019557436812304No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG4070.45098452026106134No Hit
AACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG3860.42771504869967975No Hit
AACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG3570.39558101654348615No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3520.39004066617172867No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGGATGCTTAACGCGTTAGCTAAG3240.35901470408988667No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG3210.35569049386683216No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG3180.3523662836437776No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTCTCGCGTTAGCTACG2990.33131295223109913No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATACTTAATGCGTTAGCTGCG2870.3180161113388811No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG2690.29807085000055406No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2540.28144979888528154No Hit
CACTNTTGNGAGNGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG2510.278125588662227No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATACTTAATGCGTTTGCTGCG2460.2725852382904695No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2360.26150453754695446No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2340.2592883973982515No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTGG2340.2592883973982515No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG2220.24599155650603344No Hit
CACANTTGNGTGNGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG2050.2271543652420579No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG2000.2216140148703004No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1900.2105333141267854No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTTCG1850.2049929637550279No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGCG1830.20277682360632485No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1790.19834454330891885No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG1710.18947998271410685No Hit
CACCNTTGNCGGNGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG1660.18393963234234934No Hit
TACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG1460.16177823085531928No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG1450.1606701607809678No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG1440.15956209070661628No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATACTTAACGCTTTCGCTAAG1310.14515717974004674No Hit
AACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1290.14294103959134374No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1250.13850875929393774No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTATACTTAATGTGTTAACTGCA1240.13740068921958623No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA1220.13518454907088326No Hit
TACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1190.13186033884782872No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTTCG1180.13075226877347723No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG1070.1185634979556107No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG1050.11634735780690772No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCACACTTAATCTGTTAAGTGCA1040.11523928773255622No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTACACTTAATGTGTTAACTGCA990.1096989373607987No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATAACTTTCGCTTAG990.1096989373607987No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG940.1041585869890412No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCTATTTAACGCGTTAGCTCCG940.1041585869890412No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTAATGTGTTAACGCCG930.1030505169146897No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAGTGCGTTAGCTGCG910.10083437676598668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGATC1400.0252.158877
TGCGAAC3400.0252.158877
CGCCCGT350.0252.158879
AGACTTG151.3501547E-5252.158862
CGGCCGT20600.0252.158869
CGGCCGG301.2732926E-11252.158869
CGACCGT17750.0252.158869
GACTTGC151.3501547E-5252.158863
ACGATGC100.0013521119252.158863
AGTGCGA151.3501547E-5252.158865
TAACATT100.0013521119252.158861
TGCGACC18250.0252.158867
CTTGAGA201.345743E-7252.158865
ATCTTGA151.3501547E-5252.158863
AACGATG100.0013521119252.158862
CGGTGCC151.3501547E-5252.158864
CGAGCGG201.345743E-7252.158869
ATGCGAG151.3501547E-5252.158866
GTGAGAC100.0013521119252.158866
CGGTCGT1450.0252.158869
Produced by FastQC (version 0.11.5)