FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67446
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACCTTGCGGCCGTACTCCCCAGGCGGCTGACTTAACGCGTTAGCTACG36525.414702132076031No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG33374.94766183317024No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG24383.6147436467692673No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG24023.5613676126086053No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG20062.974231236841325No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG18742.778519111585565No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG15312.2699641194437032No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG12781.894849212703496No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGGATACTTATTGCGTTCACTGCG11821.752513121608398No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG11231.6650357322895353No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA9781.4500489280313138No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG6921.026005989977167No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG6781.0052486433591317No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA6380.9459419387361742No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGCACTTAATGCGTTAACTGCG5450.8080538504877977No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG4640.6879577736263085No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG4050.600480384307446No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTACG3960.5871363757672805No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGAATACTTATTGCGTTAGCTGCG3920.5812057053049847No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG3900.5782403700738369No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG3600.5337603416066187No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3290.4877976455238265No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3190.4729709693680871No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAATGCGTTAGCTGCG3120.4625922960590695No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG3120.4625922960590695No Hit
CATCGTTGCCGACGTACTCCCCAGGTGGATAACTTAACGCTTTCGCTTAA3030.449248287518904No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG2710.40180292382053795No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGCACACTTAACGCGTTAGCTACG2550.3780802419713548No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG2460.3647362334311894No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2390.3543575601221718No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG2390.3543575601221718No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG2320.34397888681315425No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2280.33804821635085847No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA2240.3321175458885627No Hit
CACCCTTGCGAGCATACTCCCCAGGCGGTGCACTTACCGCGTTAGCTCCG2240.3321175458885627No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG2240.3321175458885627No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGCACACTTATCGCGTTTGCTTCG2230.33063487827298876No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAGTGCGTTAGCTGCG2200.32618687542626695No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG2110.31284286688610147No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTCAG2050.30394686119265785No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1900.28170684695904874No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA1850.27429350888117904No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG1830.27132817365003115No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG1710.25353616226314385No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCGCTTTCGCTTAG1680.24908815941642204No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG1650.24464015656970023No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATGCTTATTGCGTTAACTGCA1560.23129614802953474No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1480.2194348071049432No Hit
TAACCTTGCGGCCGTAGTTCCCAGGCGGCACACTTAACGCGTTGGCTCGG1440.21350413664264745No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGAGTGCTTATTGTGTTTACTTCG1420.21053880141149958No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTGCG1340.19867746048690804No Hit
TAGCCTTGCGGCCGTAGTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG1280.18978145479346442No Hit
TAACNTTGNGGCNGTACTCCCCAGGCGGCTGACTTAACGCGTTAGCTACG1270.18829878717789045No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1260.1868161195623165No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAGCGCCG1230.1823681167155947No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATAACTTAATGCTTTCGCTCTG1180.174954778637725No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG1180.174954778637725No Hit
TAACCTTGCGGCCGTAGTTCCCAGGCGGCACGCTTAACGCGTTGGCTCGG1140.16902410817542923No Hit
CAACGTTGCCGTCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG1130.1675414405598553No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTACACTTAACGCGTTTGCTCCG1130.1675414405598553No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG1070.15864543486641164No Hit
AAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1070.15864543486641164No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1070.15864543486641164No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTAATGTGTTAACGCCG1060.1571627672508377No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA1050.15568009963526377No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG980.14530142632624618No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTGCG980.14530142632624618No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGCTGACTTAACGCGTTAGCTACG970.14381875871067223No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG880.13047475017050678No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTACG880.13047475017050678No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG870.12899208255493283No Hit
CAGCCTTGCGACCGTACTACTCAGGCGGAGTACTTAATGTGTTAACTTCA840.12454407970821102No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG830.12306141209263707No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATGCTTAACGATTTCTCTGTG820.12157874447706314No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCTACTTCACGCGTTAGCTGCG780.11564807401476737No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGCTGACTTAACGCGTTAGCTACG770.11416540639919344No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG760.1126827387836195No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAACGCGTTAGCTGCG750.11120007116804553No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG750.11120007116804553No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGAATACTTATTGTGTTAACTCCG750.11120007116804553No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGGATACTTATTGCGTTCGCTGCG740.1097174035524716No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGTTCACTTAATGCGTTAGCTGCG740.1097174035524716No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG730.10823473593689767No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG710.10526940070574979No Hit
CAGTCTTGCGACCGTACTACTCAGGCGGAGTACTTAATGTGTTAACTTCA700.10378673309017585No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCTACTTCACGCGTTAGCTGCG690.10230406547460191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCCGG3150.0252.094826
AAACTTG100.0013523608252.09482
AGACTTG100.0013523608252.09482
AACCGTT301.2732926E-11252.09481
AGCCGTG100.0013523608252.09482
TCTTGAG100.0013523608252.09484
GCGCGCG100.0013523608252.09488
CGACCAT100.0013523608252.09489
GACTTGC100.0013523608252.09483
TTGAGAC100.0013523608252.09486
GAGACCG301.2732926E-11252.09488
GCCGACG400.0252.09488
ACGTGCG100.0013523608252.09484
AGACCGG151.3499668E-5252.09489
CTTGAGG151.3499668E-5252.09485
CTTGAGA100.0013523608252.09485
AGCGACC100.0013523608252.09487
CTCGCGA100.0013523608252.09485
GCGATAG100.0013523608252.09488
GTGAGAC151.3499668E-5252.09486
Produced by FastQC (version 0.11.5)