FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90212
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG61596.827251363454972No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG47535.268700394625992No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG24892.759056444818871No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG22452.488582450228351No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA20062.2236509555269808No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG18162.013035959739281No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAGTGCGTTAGCTGCG14051.5574424688511506No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG13971.5485744690285106No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTTGG13891.5397064692058706No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGCACACTTAACGCGTTAGCTCCG12501.3856249722875005No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG11881.3168979736620405No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG11571.2825344743493106No Hit
CAATCTTGCGACCGTACTCCCCAGGTGGCATACTTAATGCTTTCGCTTAG11261.2481709750365804No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG11081.2282179754356404No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTCAG10121.1218019775639605No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTAG8990.9965414800691704No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG7890.8746064825078703No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGTTTACTTATCGCTTTCGCTTCG7650.8480024830399504No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG5910.6551234868975303No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTTCG5520.6118919877621602No Hit
TAGTCTTGCGACCGTAGTCCTCAGGCGGCACACTTAACGCGTTAGCTTGG5170.5730944885381103No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG4750.5265374894692502No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA4400.4877399902452002No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG4350.4821974903560502No Hit
CAATCTTGCGATCGTACTTCCCAGGTGGAATACTTAACGCTTTCGCTTGG3810.4223384915532302No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATGCTTATTGCGTTAACTGCA3620.4012769919744602No Hit
CAGCCTTGCGACCATACTCCCCCCAGAACCCAAAGACTTTGATTTCTCAT3550.39351749212965015No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGCTGACTTAACGCGTTAGCTACG3260.3613709927725801No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTACCGCGTTAGCTACG3230.35804549283909015No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG3200.35471999290560013No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG2930.32479049350419015No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG2620.2904269941914601No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG2340.2593889948122201No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2070.22945949541081007No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2060.2283509954329801No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG1910.2117234957655301No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCGGCTTAATGCGTTAGCTGCG1780.19731299605374006No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGGATGCTTAACGCGTTAGCTAAG1750.19398749612025007No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG1740.1928789961424201No Hit
CATCNTTGNGGANGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG1690.18733649625327006No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG1550.17181749656365009No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAACGCTTTAGCTTGG1510.16738349665233007No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG1490.16516649669667008No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTCATAGGGTTACCTGCG1420.15740699685186005No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTACG1410.15629849687403005No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1380.15297299694054006No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG1360.15075599698488007No Hit
AACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1340.14853899702922008No Hit
TAATCTTGCGATCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCG1270.14077949718441007No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTATCGCTTTCGCTTGG1250.13856249722875005No Hit
AAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG1240.13745399725092006No Hit
TAGCCTTGCGGCCGTAGTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG1220.13523699729526006No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCG1220.13523699729526006No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1220.13523699729526006No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTACG1220.13523699729526006No Hit
TAATCTTGCGATCGTACTTCCCAGGCGGCACGCTTAACGCGTTAGCTCCG1220.13523699729526006No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAGCGCGTTAGCTGCG1200.13301999733960004No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1200.13301999733960004No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1200.13301999733960004No Hit
AACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA1160.12858599742828006No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG1100.12193499756130005No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG1100.12193499756130005No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1090.12082649758347005No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCATCTTCACGCGTTAGCTACG1080.11971799760564004No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTACG1070.11860949762781003No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGCACGTTTAACGCGTTAGCTTCG1070.11860949762781003No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGCACTTAATGCGTTAACTGCG1050.11639249767215006No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGAGCACTTAATGCGTTAGCTGCG1050.11639249767215006No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1000.11084999778300006No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAATTTCACGCGTTAGCTACG990.10974149780517005No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTGCG980.10863299782734005No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAACGCGTTAGCTACG960.10641599787168003No Hit
AATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG960.10641599787168003No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG910.10087349798253004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTTG201.345743E-7252.158862
AAATATT151.3501547E-5252.158861
GCCGGCG3050.0252.158868
TGCGAAC1100.0252.158867
CTTGAGA400.0252.158865
TTGCGGA8050.0252.158866
TTGCCGG3050.0252.158866
CGATCGT6950.0252.158869
GCGGGCG3650.0252.158848
ATCTTGC32550.0251.384193
GCGACCG36300.0250.422218
TGCGATC7100.0250.38317
TTGCGAT6950.0250.344776
GCGGCCG8250.0249.10248
AACCTTG15250.0248.851842
GTTGCCG3350.0248.395285
TTGCGAC36450.0248.3546
CTTGCGA45500.0248.279465
ACCTTGC15250.0248.02513
CCTTGCG29500.0247.884984
Produced by FastQC (version 0.11.5)