FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104235
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG90378.669832589821077No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG63436.085288051038519No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG48724.674053820693625No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG29652.844533985705377No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGCTGACTTAACGCGTTAGCTACG22442.1528277449992803No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG17071.6376457044179018No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG13201.2663692617642825No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA11671.119585551877968No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA10971.052429606178347No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA10290.9871924017844295No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG9220.8845397419292944No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG8800.8442461745095218No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG8420.8077900897011561No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG7980.76557778097568No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA7670.7358372907372763No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG6990.6706000863433588No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG6860.6581282678562863No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG6430.6168753297836619No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG5980.5737036504053341No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTCAG5590.5362881949441166No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGCACACTTAACGCGTTAGCTACG4410.42308245790761256No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG4320.41444812203194703No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG3750.35976399481939847No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3670.35208902959658467No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG3540.33961721110951215No Hit
CATCGTTGCCGACGTACTCCCCAGGTGGATAACTTAACGCTTTCGCTTAA3510.33673909915095696No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG3410.3271453926224397No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGCACTTAATGCGTTAACTGCG3390.3252266513167362No Hit
AAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG3220.30891735021825684No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTACACTTAACGCGTTTGCTCCG3190.30603923825970164No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTACG3180.3050798676068499No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG3140.301242384995443No Hit
CACCCTTGCGAGCATACTCCCCAGGCGGTGCACTTACCGCGTTAGCTCCG3040.2916486784669257No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3000.2878111958555188No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2940.2820549719384084No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2810.26958315345133593No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGATAACTTAACGCTTTCGCTAAG2790.26766441214563247No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATACTTAATGCGTTTGCTGCG2790.26766441214563247No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATGCTTATTGCGTTAACTGCA2740.2628675588813738No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGAATACTTATTGCGTTAGCTGCG2680.25711133496426347No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2620.251355111047153No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGTACTTAATGCGTTAGCTGCG2610.25039574039430135No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG2590.24847699908859788No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG2400.23024895668441503No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCGCTTTCGCTTAG2370.22737084472585983No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGCACACTTATCGCGTTTGCTTCG2320.22257399146160117No Hit
CAATCTTGCGATCGTACTACTCAGGCGGAGTACTTAATGCGTTAACTTCA2210.21202091428023218No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTACG2090.2005084664460114No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGTTCACTTAATGCGTTAGCTGCG2090.2005084664460114No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATCACTTAACGCGTTAGCTACG2090.2005084664460114No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2050.1966709838346045No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATGCTTAACGATTTCTCTGTG2010.1928335012231976No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2000.19187413057034586No Hit
CAACGTTGCCGTCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG1950.1870772773060872No Hit
CAGCCTTGCGGCCGTACTCCCCAGGTGGAGTGCTTAACGCTTTCGCTTGA1940.1861179066532355No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG1890.18132105338897683No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1890.18132105338897683No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTACG1840.17652420012471817No Hit
TAACCTTGCGGCCGTAGTTCCCAGGCGGCACACTTAACGCGTTGGCTCGG1790.17172734686045954No Hit
TAGCCTTGCGGCCGTAGTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG1780.1707679762076078No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG1780.1707679762076078No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1780.1707679762076078No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTAG1730.16597112294334918No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1710.16405238163764568No Hit
CAACNTTGNGGTNGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1700.16309301098479398No Hit
CACTCTTGCGAGCATACTCCCCAGGCGGAGTACTTAATGCGTTTGCTTCA1690.16213364033194225No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTACG1680.16117426967909051No Hit
CATACTTGCGTACGTACTACTCAGGCGGAGTACTTAATATGTTAACTACA1640.1573367870676836No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1600.1534993044562767No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG1570.1506211924977215No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAGCGCCG1550.14870245119201803No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCATCTTCACGCGTTAGCTACG1530.1467837098863146No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1510.1448649685806111No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGGATACTTATTGCGTTCACTGCG1510.1448649685806111No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATAACTTAATGCTTTCGCTCTG1500.1439055979277594No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAATGCGTTAGCTGCG1470.1410274859692042No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG1460.1400681153163525No Hit
CACACTTGCGTGCATACTACTCAGGCGGAGTACTTAATGCGTTAACTGCA1430.13719000335779727No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG1360.13047440878783517No Hit
TAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1330.12759629682928No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1330.12759629682928No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAACGCGTTAGCTACG1300.12471818487072481No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1300.12471818487072481No Hit
CACCCTTGCGAGCATACTCCCCAGGCGGTGCACTTATCACGTTAGCTACG1300.12471818487072481No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTACG1290.12375881421787308No Hit
TAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1270.12184007291216961No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA1260.1208807022593179No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTGCG1260.1208807022593179No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG1200.11512447834220751No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG1190.11416510768935577No Hit
TAGCCTTGCGGCCGTAGTCCTCAGGCGGTCGACTTATTGCGTTAGCTTGA1170.11224636638365233No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGCTGACTTAACGCGTTAGCTACG1150.11032762507794885No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG1120.10744951311939367No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGCG1050.10073391854943158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACG950.0251.877498
CTTGTGG100.0013569528251.877475
AGCCGTG100.0013569528251.877472
AAATATT100.0013569528251.877471
TGTGACC151.3568104E-5251.877477
TAACATT201.3542922E-7251.877471
AGTGAGA100.0013569528251.877475
AGACCGT201.3542922E-7251.877479
AGACCGG151.3568104E-5251.877479
TTGCGGA100.0013569528251.877476
GTGAGAC100.0013569528251.877476
TTGCCGG4650.0251.877476
ATAGTGA100.0013569528251.877473
CGATCGT5850.0251.877479
CGGACGT100.0013569528251.877479
TTGCGGG350.0251.877466
ATTGCGG500.0251.877465
AACCTTG33950.0250.76462
CAACCTT20500.0250.648791
GCGACCG35250.0250.448388
Produced by FastQC (version 0.11.5)