FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62331
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1756528.180199258795785No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG33415.360093693346809No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG19213.081933548314643No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG12812.0551571449198636No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG6561.052445813479649No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG6030.9674158925735189No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG6020.9658115544432145No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG5270.8454861946703888No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG5230.8390688421491713No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG4250.6818437053793458No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTACG3970.6369222377308242No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCATCTTCACGCGTTAGCTACG3420.5486836405640853No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3380.5422662880428679No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTACG3210.5149925398276941No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTACG2910.4668623959185638No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA2870.46044504339734643No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAACGCGTTAGCTACG2650.42514960453065087No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG2360.37862379875182495No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTACG2270.36418475557908586No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2240.35937174118817283No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT2160.34653703614573805No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG2090.3353066692336077No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAACGCGTTAGCTGCG1900.30482424475782516No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG1710.2743418202820426No Hit
TAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1630.2615071152396079No Hit
TAATCTTGCGACCGTACTCCCCAGGCTGTCAACTTCACGCGTTAGCTACG1580.25348542458808615No Hit
TAATCTTGCGACCGTACTCCCCCGGCGGTCAACTTCACGCGTTAGCTACG1430.22942035263352106No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCTTTAGCTACG1360.21818998572139064No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG1350.2165856475910863No Hit
TAATCGTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1340.21498130946078195No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG1300.2085639569395646No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1230.19733359002743417No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCACCTTCACGCGTTAGCTACG1220.19572925189712984No Hit
TAATCTTGCGACCGTACTCCCCAGGTGGTCAACTTCACGCGTTAGCTACG1160.18610322311530378No Hit
CAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1140.1828945468546951No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG1110.17808153246378206No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG1090.1748728562031734No Hit
TAATCTTGCGACCGTACTCCCCAGGCAGTCAACTTCACGCGTTAGCTACG1030.1652468274213473No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1010.16203815116073864No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCTCGCGTTAGCTACG1010.16203815116073864No Hit
TAATCTTGCGACCGTCCTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG990.15882947490012994No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG980.1572251367698256No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG980.1572251367698256No Hit
TAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG970.15562079863952127No Hit
TAATCTTGCGACCGTACTCCCCAGGCGATCAACTTCACGCGTTAGCTACG970.15562079863952127No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG930.14920344611830388No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG880.1411817554667822No Hit
TAATCTTGCGACCGTACTCCCCAGGCGTTCAACTTCACGCGTTAGCTACG870.13957741733647783No Hit
TAATCTTGCGACCGTACTACCCAGGCGGTCAACTTCACGCGTTAGCTACG850.13636874107586916No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGCTACG790.12674271229404308No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGCAACTTCACGCGTTAGCTACG790.12674271229404308No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACTCGTTAGCTACG780.12513837416373877No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG770.12353403603343441No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA760.12192969790313006No Hit
TAATCATGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG700.11230366912130402No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTCATAGGGTTACCTGCG690.11069933099099967No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAACGCGTTAGCTACG680.10909499286069532No Hit
TAATATTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG680.10909499286069532No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGATAACTTAATGCGTTAGCTGCG670.10749065473039097No Hit
TAATCTTGCGACCGTACTCCTCAGGCGGTCAACTTCACGCGTTAGCTACG660.10588631660008664No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTTAACTTCACGCGTTAGCTACG660.10588631660008664No Hit
TAATCTTGCGACCGGACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG650.1042819784697823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGG1100.0251.902489
ATGCGAC550.0251.902486
TCTTGAG251.3515091E-9251.902474
GCGACGG151.3535837E-5251.902478
TTGTGAC100.001355204251.902476
AAATAAA100.001355204251.902471
CGACGGA100.001355204251.902479
CGTGCGA1050.0251.902475
CGACCGA251.3515091E-9251.902479
TGTGACC100.001355204251.902477
TTGAGAC251.3515091E-9251.902476
GAGACCG251.3515091E-9251.902478
TGCGACG100.001355204251.902477
CTTGAGA251.3515091E-9251.902475
TTGCGGT251.3515091E-9251.902476
TTGCGAT350.0251.902476
CCGACCG251.3515091E-9251.902478
CGGTCGT251.3515091E-9251.902479
ATAAAGA100.001355204251.902473
ATTGCGA800.0251.902475
Produced by FastQC (version 0.11.5)