FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64592
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATCTTGCGACCGTACTCCCCAGGTGGCATACTTAATGCTTTCGCTTAG1022315.82703740401288No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG34525.344315085459499No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG25984.0221699281644785No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG25854.002043596730245No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTTCG19893.0793287094377013No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG12781.9785731979192471No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG12181.8856824374535546No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG11511.7819544216001983No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTTGG11081.7153827099331185No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG8831.3670423581867723No Hit
AAATCTTGCGACCGTACTCCCCAGGTGGCATACTTAATGCTTTCGCTTAG6310.9769011642308645No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG5440.8422095615556106No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAGTGCGTTAGCTGCG5350.8282759474857567No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAGTGCGTTAGCTGCG4730.7322888283378747No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAGAACTTAGCGCGTTAGCTTCG4680.7245479316324003No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG3420.5294773346544464No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG3130.4845801337626951No Hit
CAATNTTGNGACNGTACTCCCCAGGTGGCATACTTAATGCTTTCGCTTAG3050.4721946990339361No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG2930.4536165469407976No Hit
TAATCTTGCGACCGTACTCCCCAGGTGGCATACTTAATGCTTTCGCTTAG2830.43813475352984893No Hit
CAATCTTGCGACCGTACTCCCCAGGTGGCATACTTAATGCTTTCGCTTCG2520.39014119395590785No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGCACACTTAACGCGTTAGCTCCG2400.3715630418627694No Hit
CAATCTTGCGATCGTACTTCCCAGGTGGATTACTTAACGCTTTCGCTTAG2370.36691850383948477No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG1930.2987986128313104No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTACG1790.27712410205598215No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1770.27402774337379243No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAACGCGTTAGCTGCG1690.26164230864503346No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1670.2585459499628437No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTTTACTTCTCGCGTTAGCTTCG1540.23841961852861035No Hit
TAGCCTTGCGGCCGTAGTCCTCAGGCGGCATACTTAACGCGTTAGCTTGG1490.23067872182313598No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG1480.2291305424820411No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAATACTTAACGCGTTAGCTACG1450.2244860044587565No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG1350.20900421104780778No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCTACTTCACGCGTTAGCTGCG1190.1842333415902898No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG1160.1795888035670052No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1150.17804062422591033No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG1070.16565518949715136No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTCATAGGGTTACCTGCG1050.1625588308149616No Hit
TAATCTTGCGATCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG1030.15946247213277187No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAACGCGTTAGCTACG1020.15791429279167699No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG1020.15791429279167699No Hit
TAGTCTTGCGACCGTAGTCCTCAGGCGGCACACTTAACGCGTTAGCTTGG1000.15481793410948724No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG990.15326975476839239No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAATTTCACGCGTTAGCTACG990.15326975476839239No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAACACTTAACGCGTTAGCTACG970.15017339608620264No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCATGCGTTAGCTGCG950.1470770374040129No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG940.14552885806291802No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG940.14552885806291802No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG930.14398067872182313No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG900.1393361406985385No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG870.1346916026752539No Hit
CAATCTTGCGACCGTACTCCCCAGTTGGCATACTTAATGCTTTCGCTTAG870.1346916026752539No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGTATGCTTATCAGATTCCCTACG850.13159524399306416No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG840.13004706465196927No Hit
AAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG830.12849888531087442No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGCTGCG830.12849888531087442No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG810.12540252662868467No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGTTTACTTATCGCTTTCGCTTCG770.11920980926430517No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATGCGTTAGCTGCG770.11920980926430517No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGCTACTTAACGCGTTAGCTTCG760.1176616299232103No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAATGCGTTAGCTGCG730.11301709189992569No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCTCGCGTTAGCTGCG730.11301709189992569No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTCGCTGCG670.10372801585335645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGT201.3459794E-7252.035921
CGTGCGG201.3459794E-7252.035925
GCGAACG400.0252.035928
GCGCGCG151.3509549E-5252.035928
CGCGCGT100.0013531715252.035929
CCGTGCG201.3459794E-7252.035924
TGCGCGC151.3509549E-5252.035927
GCGGGCG1200.0252.035928
AGTCTTG2850.0252.035922
TTGCGGT600.0252.035926
GTGAGAC100.0013531715252.035926
ACCGTGC151.3509549E-5252.035923
ATTGCGG100.0013531715252.035925
AGAGCGG100.0013531715252.035929
AAGCTTG100.0013531715252.035922
AAGTCTT151.3509549E-5252.035921
CGGCCGT7800.0252.03599
GCCGGCG1450.0252.03598
TTGCGGA450.0252.03596
TTGCCGG1350.0252.03596
Produced by FastQC (version 0.11.5)