FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86421
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1091012.624246421587346No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG77648.983927517617246No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG45215.231367375985004No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG37364.323023339234677No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT24752.863887249626827No Hit
CAATCTTGCGACCGTACTCCCCAGGTGGCATACTTAATGCTTTCGCTTAG11631.3457377257842422No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGCACACTTAACGCGTTAGCTCCG8991.0402564191573807No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG7850.9083440367503269No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG7710.8921442704898116No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG6480.7498177526295692No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA4980.5762488284097614No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG4650.5380636650814038No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG4380.5068212587218384No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG3900.4512792029714999No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG3770.43623656287244994No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG3270.37838025479918075No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3120.36102336237719995No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3060.3540806054084077No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG2880.33325233450203073No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2860.3309380821791No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTACG2520.2915957926892769No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTACG2510.29043866652781153No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG2480.28696728804341537No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA2310.2672961432985038No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2260.2615105124911769No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG2260.2615105124911769No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG2240.25919626016824615No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG2220.25688200784531534No Hit
AAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT2110.24415362006919616No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGATAACTTAATGCGTTAGCTGCG2080.2406822415848No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAACGCGTTAGCTACG2050.23721086310040385No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCATCTTCACGCGTTAGCTACG1930.22332534916281924No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATACTTAATGCGTTTGCTGCG1890.2186968445169577No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTACG1860.21522546603256154No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTCATAGGGTTACCTGCG1720.19902569977204615No Hit
CACTCTTGCGAGCATACTCCCCAGGCGGAGTACTTAATGCGTTTGCTTCA1700.19671144744911537No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1700.19671144744911537No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCG1660.19208294280325383No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1660.19208294280325383No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAACGCGTTAGCTACG1530.17704030270420384No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGCACTTAATGCGTTAACTGCG1460.16894041957394615No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG1420.1643119149280846No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1410.1631547887666192No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTACG1340.15505490563636154No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTGCG1330.15389777947489613No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1290.1492692748290346No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1240.14348364402170768No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG1180.13654088705291537No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTGCG1160.1342266347299846No Hit
TAATCTTGCGACCGTACTCCCCAGGTGGCATACTTAATGCTTTCGCTTAG1150.13306950856851923No Hit
TAGTCTTGCGACCGTACTTCCCAGGCGGAGCACTTAACGCGTTAGCTGCG1150.13306950856851923No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTCGCTGCG1140.13191238240705383No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1100.12728387776119232No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTACTGCGTTAGCTGCG1090.12612675159972692No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTACG1090.12612675159972692No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGAAGCTTAATGCGTTAGCTGCG1080.12496962543826153No Hit
TAATCTTGCGATCGTACTTCCCAGGCGGCACGCTTAACGCGTTAGCTCCG1010.11686974230800384No Hit
TAATCTTGCGACCGTACTCCCCAGGCTGTCAACTTCACGCGTTAGCTACG940.10876985917774615No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCTTTAGCTACG940.10876985917774615No Hit
TAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG930.10761273301628077No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATATCTTAATGCGTTAGCTGCG900.10414135453188463No Hit
TAATCTTGCGATCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG890.10298422837041923No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTGCG870.10066997604748847No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATAGA100.0013605644251.628591
GCCGGCG201.3596218E-7251.628598
AAGAATG100.0013605644251.628592
TTGAGAC400.0251.628596
AGTGCGA201.3596218E-7251.628595
AACATTG100.0013605644251.628592
GCGGGCG201.3596218E-7251.628598
AGACCGT350.0251.628599
CTTGAGA350.0251.628595
TTGCCGG201.3596218E-7251.628596
AATAGTG100.0013605644251.628592
ATGTTGC100.0013605644251.628593
TAGTCCG100.0013605644251.628594
TGCCGGC201.3596218E-7251.628597
TCTTGAG301.4551915E-11251.628574
TGAGACC500.0251.628567
GCGACCG55200.0251.172748
ATCTTGC48150.0250.583373
TTGCGAC57150.0250.527836
CGACCGT54400.0250.472189
Produced by FastQC (version 0.11.5)