FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66284
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG952114.363949067648301No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG769411.60762778347716No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG59408.961438657896323No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG29064.384165107718303No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG16232.4485547040009656No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG11231.694224850642689No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG7621.1495986965180134No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG6701.0108020035000906No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG5640.8508840745881359No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG5470.8252368595739545No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG3510.5295395570575101No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG3240.4888057449761632No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2970.4480719328948162No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2610.39376018345302033No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG2400.3620783296119727No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2330.3515177116649568No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG2190.3303964757709251No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTACG2090.3153098787037596No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTACG2070.31229255929032645No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG1860.28061070544927885No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1850.2791020457425623No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTACG1790.270050087502263No Hit
AACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG1770.2670327680888299No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1680.25345483072838093No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1630.24591153219479814No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCATCTTCACGCGTTAGCTACG1510.22780761571419952No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAACGCGTTAGCTACG1430.21573833806046708No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG1430.21573833806046708No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGATAACTTAATGCGTTAGCTGCG1420.21422967835375054No Hit
CAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1210.1825478245127029No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTTCG1200.18103916480598636No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG1160.17500452597912017No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG1130.1704785468589705No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTACG1110.16746122744553738No Hit
CAACCTTGCGGCCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTTCG1060.15991792891195464No Hit
CAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1040.15690060949852153No Hit
TAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1030.15539194979180496No Hit
CACTNTTGNGAGNGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG1010.15237463037837184No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG960.14483133184478908No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATTACTTAATGCGTTAGCTGCG930.14030535272463943No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTACG890.1342707138977732No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGAAGCTTAATGCGTTAGCTGCG880.13276205419105666No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG880.13276205419105666No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTACTGCGTTAGCTGCG870.13125339448434012No Hit
CAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG860.12974473477762355No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCTTTAGCTACG860.12974473477762355No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG840.12672741536419047No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTCGCTGCG830.1252187556574739No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG810.1222014362440408No Hit
TAACCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG780.11767545712389113No Hit
TAATCTTGCGACCGTACTCCCCAGGCGATCAACTTCACGCGTTAGCTACG760.11465813771045802No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG750.11314947800374146No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCACCTTCACGCGTTAGCTACG740.11164081829702491No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCT730.11013215859030838No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG720.10862349888359182No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG700.10560617947015871No Hit
TAATCTTGCGACCGTACTCCCCCGGCGGTCAACTTCACGCGTTAGCTACG690.10409751976344216No Hit
CAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG670.10108020035000904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTG100.0013443036252.594712
TCTTGAG201.3314457E-7252.594714
CGTGAGA201.3314457E-7252.594715
ATCGTGA201.3314457E-7252.594713
TCGTGAG201.3314457E-7252.594714
CTTGAGA201.3314457E-7252.594715
CGAGCGA100.0013443036252.594719
AAGTCTT100.0013443036252.594711
ACCATGC151.339218E-5252.59473
AGTGCGA151.339218E-5252.59475
AGACCGG151.339218E-5252.59479
CCGACCG151.339218E-5252.59478
AGCCTTG650.0252.59472
GTGAGAC151.339218E-5252.59476
TTGCCGG151.339218E-5252.59476
GTTGCGG151.339218E-5252.59475
TGCCGGC151.339218E-5252.59477
GCGGCCG16950.0251.849588
CTTGCGG16850.0251.845175
TTGCGGC16550.0251.831596
Produced by FastQC (version 0.11.5)