FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8941946
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG162455918.16784623839151No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG6482087.249070839837324No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG4198704.69551035087888No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG3490573.903591008042321No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3259123.644754732359153No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG3253083.638000050548281No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1485491.6612603117934284No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1271311.4217375054602208No Hit
CACTCTTGCGAGCATACTCCCCAGGCGGAGTACTTAATGCGTTTGCTTCA689380.7709507527779746No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG526580.5888874748293045No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG482020.5390549216020764No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG418930.46849980977295097No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG416380.4656480815249835No Hit
TAGTCTTGCGACCGTACTCCCCAGTCGGTCTACTTATCGCGTTAGCTGCG401020.448470612548991No Hit
AACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG347080.3881481726684549No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA339310.37945878894817753No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTACG329080.36801832621221375No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG325500.364014723417028No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG297980.3332384248350415No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG275430.3080202005245838No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAGCGCCG270120.3020818958199926No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG259360.2900487209383729No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG255980.2862687831038121No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGAAGCTTAATGCGTTAGCTGCG247890.2772215354465348No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTCGCTGCG240980.2694939110569444No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG207370.23190701442392966No Hit
TAGTCTTGCGACCGTACTCCCCATGCGGTCTACTTATCGCGTTAGCTGCG204770.2289993699358059No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAGCTGCG199640.22326236369577718No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG195560.21869959849902917No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG194480.21749180771165472No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG193160.21601561897153038No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG185650.20761699970006528No Hit
TAGCCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG175110.19582985627513297No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG173190.1936826726531339No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG171030.19126709107838497No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG169100.18910872420835464No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTGCG161880.18103441912979568No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG144770.1618998817483353No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG130380.1458071878313736No Hit
TACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG124300.1390077730283766No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG118130.13210770899309837No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT117620.13153736334350485No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTCTCGCGTTAGCTGCG114600.12816002243806884No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTACTTAATGCGTTAGCTACG111590.12479386478066407No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG109690.12266904765472751No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG109640.12261313141457128No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGCG109010.12190858678860284No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCT109000.1218974035405716No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG107710.12045476454454099No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCTTTAGCTGCG106540.1191463245248853No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCG105660.11816219869813573No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTGCG100650.11255939143448193No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG99610.11139633363923246No Hit
TAGTCGTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG99340.11109438594238882No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATTGCGTTAGCTGCG98770.11045694080460786No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG98190.10980831241879566No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG95180.10644215476139088No Hit
TAGTCTTGCGACCGTCCTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG94280.10543566243857881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT4867450.0251.535819
GCGACCG4950150.0251.286458
TAGTCTT2331900.0250.973141
TGCGAGC1087700.0250.852787
AGTCTTG2526750.0250.820372
GTCTTGC2527650.0250.3373
TGCGACC4989250.0250.307797
TTGCGAC4858950.0250.296956
GCGGCCG1243500.0249.08038
CTTGCGA6176550.0248.919045
CGGCCGT1225750.0248.87119
AACCTTG1395850.0248.63092
CGATCGT242000.0248.567759
TCTTGCG5870200.0248.532824
GCGAGCG1004900.0248.248088
CTTGCGG1437450.0248.154245
TTGCCGG100750.0247.907796
TGCGGCC1248950.0247.85217
ATCTTGC2268200.0246.659583
TTGCGAG1084000.0246.626226
Produced by FastQC (version 0.11.5)