FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95826
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG68257.122284140003757No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG65556.8405234487508615No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG53575.590340826080604No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG41474.3276355060213305No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG39944.167971114311356No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA36123.769331914094296No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG25442.6548118464717296No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG15801.6488218228873166No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG15721.640473357961305No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA13031.3597562248241606No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG11691.2199194373134639No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG10521.0978231377705423No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG10171.0612986037192411No Hit
CAACGTTGCCGTCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG7390.7711894475403335No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG6750.7044017281322397No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA6070.63343977626114No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG5080.5301275228017448No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA4410.4602091290463966No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG4030.42055392064784086No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG3690.38507294471229103No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG3670.3829858284807881No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG3390.353766201239747No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG3000.31306743472543985No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG2810.293239830526162No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG2770.2890655980631561No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT2710.28280424936864734No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2710.28280424936864734No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTACG2560.2671508776323753No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGCACTTAATGCGTTAACTGCG2500.26088952893786654No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG2370.2473232734330975No Hit
AACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG2300.2400183666228372No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG2280.2379312503913343No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2120.22123432053931083No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG2090.2181036461920564No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2000.20871162315029324No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1940.20245027445578442No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG1930.201406716340033No Hit
TAGTNTTGNGACNGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1890.19723248387702713No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGCTGACTTAACGCGTTAGCTACG1880.19618892576127564No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG1820.18992757706676686No Hit
TAGTCTTGCGACCGTACTCCCCAGTCGGTCTACTTATCGCGTTAGCTGCG1690.1763613215619978No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTACG1610.16801285663598606No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATAACTTAATGCTTTCGCTCTG1590.1659257404044831No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTACG1550.16175150794147727No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGCG1530.15966439170997435No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG1520.15862083359422285No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTACG1500.15653371736271993No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG1490.15549015924696846No Hit
AAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1460.15235948489971407No Hit
CACCNTTGNCGGNGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG1340.13983678751069648No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1330.138793229394945No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1260.13148832258468474No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTACACTTAATGTGTTAACTGCA1240.1294012063531818No Hit
CATCGTTGCCGACGTACTCCCCAGGTGGATAACTTAACGCTTTCGCTTAA1240.1294012063531818No Hit
CACTNTTGNGAGNGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG1220.12731409012167888No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGGATACTTATTGCGTTCACTGCG1200.12522697389017595No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTATACTTAATGTGTTAACTGCA1150.1200091833114186No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1140.11896562519566715No Hit
TAATCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1060.11061716026965542No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCATCTTCACGCGTTAGCTACG1020.10644292780664956No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAACGCGTTAGCTACG1010.10539936969089808No Hit
CAGCCTTGCGGCCGTACTCCCCAGGTGGAGTGCTTAACGCTTTCGCTTGA1010.10539936969089808No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG980.10226869534364369No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTGA100.0013550443251.984975
TCTTGAG400.0251.984974
ACATTGC201.3508361E-7251.984973
AACATTG201.3508361E-7251.984972
TCCGTCG100.0013550443251.984978
CGATGCC100.0013550443251.984974
ATGCCGG100.0013550443251.984976
AACAGTG201.3508361E-7251.984972
GAGGGAG100.0013550443251.984978
TAGTCGT100.0013550443251.984971
CGGCCGG301.4551915E-11251.984959
CGACCGG850.0251.984959
GCCGGCG6800.0251.984958
CGACCAT600.0251.984959
TCTAGCG151.3541499E-5251.984954
AGACCGT600.0251.984959
AATCTAG151.3541499E-5251.984952
TCGACCG301.4551915E-11251.984958
CCTTGAG151.3541499E-5251.984954
CTAGCGA151.3541499E-5251.984955
Produced by FastQC (version 0.11.5)