FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63436
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCA35625.615108140488051No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG34485.435399457721168No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA34305.407024402547449No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG27374.314584778359292No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG25033.945709061100952No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG15492.441831136893877No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG13412.11394161044202No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTAATGTGTTAACGCCG13342.102906866763352No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG9261.4597389494924018No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG8851.3951068793744876No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG7991.2595371713222776No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG7981.2579607793681822No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG7091.1176618954536857No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGCACTTAATGCGTTAACTGCG6310.9947033230342393No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAGCGCCG5980.9426823885490888No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG5850.9221892931458479No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG5630.8875086701557476No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG5140.8102654644050697No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG4950.7803140172772558No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTGCG4640.7314458667002963No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAGCTGCG4610.7267166908380099No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG4020.6337095655463775No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG3530.5564663597956996No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTACG3360.5296676965760767No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA3150.4965634655400719No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG2980.469764802320449No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTACTTATTGTGTTAACGACG2720.42877861151396685No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2430.3830632448451983No Hit
AAGCCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCA2380.375181285074721No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG2360.37202850116653No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG2080.327889526451857No Hit
CAACCTTGCGGTCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG2060.324736742543666No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2030.32000756668137964No Hit
AAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG2000.31527839081909326No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1960.3089728230027114No Hit
AACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1810.2853269436912794No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGGTACTTAGTGTGTTAACGACG1750.2758685919667066No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1640.25852828047165644No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAATACTTATTGCGTTAGCTCCG1620.25537549656346553No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG1580.2490699287470837No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGAATACTTATTGCGTTAGCTGCG1510.2380351850684154No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG1500.23645879311431994No Hit
CAGCCTTGCGACCGTACTACTCAGGCGGAGTACTTAATGTGTTAACTTCA1470.23172961725203356No Hit
AAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG1430.2254240494356517No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG1420.2238476574815562No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG1320.20808373794060156No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAACTGCG1270.20020177817012424No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAATGCGTTAACTGCG1230.19389621035374235No Hit
CAGCNTTGNGGCNGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCA1170.18443785862916956No Hit
CAGTNTTGNGACNGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG1100.1734031149505013No Hit
CAATCTTGCGATCGTACTCCCCAGGTGGAATACTTATTGTGTTAACTGCG1080.17025033104231035No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG1040.1639447632259285No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTCCG1030.162368371271833No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTGCACTTAATGCGTTAGCTGCG1010.1592155873636421No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTATTGTGTTAACTGCG1000.15763919540954663No Hit
AACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG1000.15763919540954663No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG1000.15763919540954663No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAACGCTTTCGCTTGG1000.15763919540954663No Hit
CACTCTTGCGAGCATACTCCCCAGGCGGAGTACTTAATGCGTTTGCTTCA980.1544864115013557No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGGTACTTACTGTGTTTACGACG980.1544864115013557No Hit
CACANTTGNGTGNATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA960.15133362759316477No Hit
CAGCNTTGNGACNGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG910.14345166782268742No Hit
CAGTCTTGCGACCGTACTTCCCAGGCGGCGGACTTAACAGCTTCCCTTCG900.14187527586859197No Hit
CAATCTTGCGATCGTACTCCCCAGGCGGAATGCTTATCGTGTTTACGTCG850.13399331609811463No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG850.13399331609811463No Hit
CACACTTGCGTGCATACTACTCAGGCGGAGTACTTAATGCGTTAACTGCA830.1308405321899237No Hit
CAACCTTGCGGCCGTACTTCCCAGGCGGGATACTTAATACGTTAGCTCCG820.12926414023582822No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTATTGCGTTTGCTGCG810.12768774828173277No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG800.1261113563276373No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG760.11980578851125544No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA750.11822939655715997No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATACTTAATGCGTTAGCTGCG740.1166530046030645No Hit
AACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTAATGTGTTAACGCCG730.11507661264896904No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG720.11350022069487357No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA720.11350022069487357No Hit
CAGCCTTGCGACCGTACTACTCAGGTGGGATACTTAATGTGTTAACTTCA710.1119238287407781No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTCTCGCGTTAGCTACG660.10404186897030078No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTATCGTGTTAACTTCG660.10404186897030078No Hit
CACTNTTGNGAGNGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG660.10404186897030078No Hit
CAGCCTTGCGACCGTACTACTCAGGCGGAGTACTTAATACGTTAGCTTCA640.10088908506210985No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGGTGCTTATCGCGTTAGCTACG640.10088908506210985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT20700.0252.16759
AAACCTT750.0252.16751
AAACTTG251.3424142E-9252.167482
AGCCGTG151.3480241E-5252.167482
GCGACCG21350.0252.167488
CGTGCGA151.3480241E-5252.167485
CGGCCGT17200.0252.167489
CGACCGG500.0252.167489
CGCTCGT500.0252.167489
AACATTG251.3424142E-9252.167482
TTGCGAT1650.0252.167486
CTCGCGA151.3480241E-5252.167485
ATGCGGC151.3480241E-5252.167486
ATTGCGG151.3480241E-5252.167485
ATTGCGA350.0252.167485
GCGCTCG500.0252.167488
GGCCTTG151.3480241E-5252.167482
TAGTCTT2300.0252.167481
GCGAGCG6050.0252.167488
TCTTGAG100.0013509986252.167474
Produced by FastQC (version 0.11.5)