FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137576
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG80995.886927952549863No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA63214.594551375239867No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG59274.308164214688608No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG51703.7579228935279407No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG38912.8282549281851486No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG35222.5600395417805433No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG32822.3855905099726695No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG30322.203872768506135No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG25741.8709658661394428No Hit
CAGCCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCA25041.8200848985288132No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG21121.5351514799092865No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAGCACTTAATGCGTTAACTGCG16041.1659010292492877No Hit
CACTCTTGCGAGCGTACTTCCCAGGCGGAGTACTTAATGTGTTAACTGCG14921.08449148107228No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG13941.0132581264173985No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTG13310.9674652555678317No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTAATGTGTTAACGCCG10760.782113159271966No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTACTTATTGCGTTAGCGCCG8110.5894923533174391No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG7350.5342501599116125No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTGCG7090.5153515147990928No Hit
CACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAGCTGCA6910.5022678374135023No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTACG6670.484822934232715No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAATGCTTTCGCTTGG6420.46665116008606156No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTACACTTAATGTGTTAACTGCA6180.44920625690527416No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG5960.43321509565621913No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTGTA5900.42885386986102225No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGGATACTTATTGCGTTCACTGCG5650.41068209571436876No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG5040.3663429667965343No Hit
CACTCTTGCGAGCGTACTCCCCAGGCGGAGTACTTATTGCGTTAGCTGCG4980.36198174100133745No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTTTCGCTTGG4460.3241844507762982No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG4350.31618887015177066No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG4350.31618887015177066No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCG4200.30528580566377855No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGCACACTTAACGCGTTAGCTACG4070.29583648310751876No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGAATACTTATTGCGTTAGCTGCG4070.29583648310751876No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCTCGCGTTAGCTTCG3960.2878409024829912No Hit
AACACTTGCGTGCATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA3720.27039599930220387No Hit
CAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAGCTGCG3670.2667616444728732No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG3640.26458103157527474No Hit
CACACTTGCGTGCATACTACTCAGGCGGAGTACTTAATGCGTTAACTGCA3580.2602198057800779No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGGATGCTTAACGCTTTCGCTTGG3570.25949293481421176No Hit
AACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG2870.20861196720358202No Hit
TAGTNTTGNGACNGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG2700.19625516078385766No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2450.17808338663720416No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG2410.1751759027737396No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2370.17226841891027506No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGGGTACTTATTGCGTTAACTCCG2350.17081467697854277No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG2330.1693609350468105No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTCCG2160.15700412862708613No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG2100.15264290283188928No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAACTGCG2090.15191603186602315No Hit
TAGTCTTGCGACCGTACTCCCCAGTCGGTCTACTTATCGCGTTAGCTGCG2060.14973541896842474No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGCG2060.14973541896842474No Hit
AACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG2050.14900854800255858No Hit
CACACTTGCGTGCATACTACTCAGGCGGAGTACTTATTGCGTTAACTGCA2000.1453741931732279No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG1960.14246670930976332No Hit
CAACCTTGCGGTCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG1940.14101296737803107No Hit
CACTCTTGCGAGCATACTCCCCAGGCGGAGTACTTAATGCGTTTGCTTCA1930.1402860964121649No Hit
CAACCTTGCGGCCGTACTCCCCAGGTGGATTACTTATTGTGTTAACTGCG1930.1402860964121649No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG1880.13665174158283422No Hit
AAGTCTTGCGACCGTAGTCCCCAGGCGGGATACTTAACGCGTTAACTGCG1840.13374425771936965No Hit
CAGCCTTGCGACCGTACTACTCAGGCGGAGTACTTAATGTGTTAACTTCA1820.13229051578763737No Hit
AAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAACGCGTTAACTACG1780.12938303192417283No Hit
CAACNTTGNGATNGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1750.1272024190265744No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1750.1272024190265744No Hit
AAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG1710.12429493516310985No Hit
CACANTTGNGTGNATACTCCCCAGGCGGAATACTTATTGCGTTAACTGCA1700.12356806419724371No Hit
CACCNTTGNCGGNGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTGTG1700.12356806419724371No Hit
AAGCCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCA1680.12211432226551143No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG1650.11993370936791302No Hit
AAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1560.11339187067511776No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1480.10757690294818864No Hit
CACTNTTGNGAGNGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG1410.10248880618712566No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGGATGCTTAATGCGTTAACTGCG1390.10103506425539338No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTACTTATTGTGTTAACGACG1380.10030819328952724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGTG151.3581719E-5251.85932
GCGGGCG301.4551915E-11251.85938
ATTGCGA650.0251.85935
GTGCGAC650.0251.85936
TCTTGAG251.3624231E-9251.859284
CTTGAGA251.3624231E-9251.859285
GCGAGCG10950.0251.859288
AGCCATG100.0013577315251.859272
TGCGGGC400.0251.859277
TTGAGAC201.3564022E-7251.859276
CGAGCGG100.0013577315251.859279
GTGCGGG100.0013577315251.859276
GTGCGAG201.3564022E-7251.859276
AAGTGCG100.0013577315251.859274
TGAGAGC100.0013577315251.859277
AGAGCGA100.0013577315251.859275
GTAGTGC100.0013577315251.859273
CGACCGT42250.0250.96519
TGCCGGC10700.0250.682367
GCCGGCG10650.0250.676838
Produced by FastQC (version 0.11.5)