FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75741
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG56347.438507545450945No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG42615.625750914300049No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG36434.809812386950265No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG34274.524629989041602No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG20302.6801864247897442No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG14891.965910141138881No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG12321.6265958991827412No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCG8071.0654731255198637No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG7751.023223881385247No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG7360.971732615096183No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG5660.7472835056310321No Hit
CAGCCTTGCGACCATACTCCCCCCAGAACCCAAAGACTTTGATTTCTCAT5540.7314400390805508No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAGTGCGTTAGCTGCG5400.712955994771656No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAGAACTTAGCGCGTTAGCTTCG5370.7089951281340356No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTTGG5160.6812690616706936No Hit
CAATCTTGCGACCGTACTCCCCAGGTGGCATACTTAATGCTTTCGCTTAG5010.661464728482592No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG4920.649582128569731No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG4810.6350589508984565No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG4390.5796068179717723No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCGACG4260.5624430625420842No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTATCGCGTTAGCTACG4260.5624430625420842No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG4140.546599595991603No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTATCGCTTTCGCTAAG3990.5267952628035014No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCG3940.5201938184074675No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGCACACTTAACGCGTTAGCTCCG3500.4621011077223697No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG3450.4554996633263358No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3240.4277735968629936No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTACCGCGTTAGCTACG3000.3960866637620311No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGGGCACTTCACGGTTTCCCTTCG2930.3868446416075837No Hit
CAATCTTGCGATCGTACTTCCCAGGTGGATTACTTAACGCTTTCGCTTAG2790.3683605972986889No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTATCGCGTTAACTTCG2560.33799395307693325No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGAGAACTTAACGCGTTAGCTTCG2500.3300722198016926No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGTATGCTTATCAGATTCCCTACG2470.3261113531640723No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG1980.2614171980829405No Hit
TAGCCTTGCGGCCGTAGTCCTCAGGCGGCACACTTAACGCGTTAGCTTGG1960.25877662032452703No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1920.2534954648076999No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTTAG1830.241612864894839No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAGTGCGTTAGCTGCG1820.24029257601563223No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCTACTTCACGCGTTAGCTGCG1770.23369113161959837No Hit
CAGCCTTGCGACCGTACTCCCCAGGCGGCATACTTAACGCGTTAGCTCCG1700.22444910946515095No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAACGCTTTAGCTTGG1670.22048824282753068No Hit
CAACCTTGCGGCCGTACTTCCCAGGCGGAAGACTTAACGCGTTAGCTAAT1660.21916795394832392No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG1570.20728535403546294No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTCATAGGGTTACCTGCG1560.20596506515625618No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1520.20068390963942911No Hit
TAGTCTTGCGACCGTAGTCCTCAGGCGGCACACTTAACGCGTTAGCTTGG1480.19540275412260202No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1480.19540275412260202No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG1480.19540275412260202No Hit
TAGCCTTGCGGCCGTAGTCCTCAGGCGGCATACTTAACGCGTTAGCTTGG1470.19408246524339526No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAACGTGTTAACTTCG1440.19012159860577496No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG1430.18880130972656817No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCTACTTCACGCGTTAGCTGCG1400.18484044308894787No Hit
CAGACTTGCGTACCATACTTCCCCCAGAACCCAAAGACTTTGATTTCTCA1390.18352015420974108No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1340.17691870981370725No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTTACTGCA1340.17691870981370725No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1320.1742781320552937No Hit
TAATCTTGCGACCGTAGTCCCCAGGCGAAGTGCTATTCACGTTAGCTATG1320.1742781320552937No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTAACGCGTTAGCTGCG1280.16899697653846663No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG1260.16635639878005307No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTAATGCGTTAGCTGCG1150.1518332211087786No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTGCG1130.14919264335036508No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG1130.14919264335036508No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1090.14391148783353797No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGGGCACTTCACGGTTTCCCTGCG1070.14127091007512443No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTACG1020.13466946567909058No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG1000.13202888792067705No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG1000.13202888792067705No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATGCGTTAGCTGCG990.13070859904147025No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTATCGCGTTAGCTTCG990.13070859904147025No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG960.12674773240384996No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGCG960.12674773240384996No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCATGCGTTAGCTGCG940.12410715464543642No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGTTTAATGCGTTAGCTGCG920.12146657688702288No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGTAAACTTAACGCTTTCGCTTGG880.1161854213701958No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTCGCTGCG860.11354484361178227No Hit
TAATCTTGCGACCGTACTCCCCATGCGGTCAACTTCACGCGTTAGCTGCG850.11222455473257548No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGCGCTTACCGCGTTAGCTACG850.11222455473257548No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCTCGCGTTAGCTGCG820.10826368809495517No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGATAACTTAATGCGTTAGCTGCG820.10826368809495517No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG810.1069433992157484No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG810.1069433992157484No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAGAACTTATCGCGTTAGCTGCG800.10562311033654165No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGCTGCG800.10562311033654165No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGCG790.10430282145733485No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCATGCGTTAGCTGCG780.10298253257812809No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG770.10166224369892132No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAAGACTTAATGCGTTAGCTGCG760.10034195481971456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCG30700.0251.781788
CGTGCGG151.357363E-5251.781785
CGTGCGA600.0251.781785
CGGCCGG201.3543468E-7251.781789
CCGTGCG201.3543468E-7251.781784
CGACCGG350.0251.781789
GAGACCG201.3543468E-7251.781788
AACATTG251.3587851E-9251.781782
TGCGAAC500.0251.781787
AGACCGT151.357363E-5251.781789
TTGCGGT4250.0251.781786
GTGAGGC100.0013577442251.781786
ATTGCGG251.3587851E-9251.781785
GTGCGGC151.357363E-5251.781786
AAGTCTT251.3587851E-9251.781781
GCGAACG450.0251.781778
TTGCCGG1450.0251.781776
CGACCGT30400.0251.367689
TGCGGCC21300.0250.59977
GCGGCCG21250.0250.596948
Produced by FastQC (version 0.11.5)