FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64529
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG859813.324241813758157No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG39406.105781896511646No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAGTGCGTTAGCTGCG26874.164019278154008No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG20723.210959413596987No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGGGCACTTCACGGTTTCCCTTCG16552.564738334702227No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGGGCACTTCACGGTTTCCCTGCG9861.5279951649645895No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTTAG8191.2691967952393497No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG7901.2242557609756854No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG7121.103379875714795No Hit
TAATCTTGCGATCGTACTTCTCAGGCGAAATGTTTAACGTGTTAACTGTA6180.9577089370670552No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG5730.8879728494165413No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTTTCAT4960.768646654992329No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG4440.6880627314850687No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGAAGCTTAATGCGTTAGCTGCG4080.6322738613646578No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTCATAGGGTTACCTGCG4020.6229757163445893No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATTATTTAATGCGTTAGCTGCG3800.5888825179376714No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG3040.47110601435013716No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG3020.468006632676781No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG2880.44631096096328776No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG2800.4339134342698631No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2730.42306559841311664No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG2690.41686683506640426No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG2580.39982023586294535No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG2210.3424816749058563No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTAACGCGTTAGCTGCG2210.3424816749058563No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGAGCACTTAACACTTTCGCTACG2170.33628291155914397No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTATCGCGTTAGCTACG2090.3238853848657193No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGATAACTTAATGCGTTAGCTGCG2060.319236312355685No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGCACACTTAACGCGTTAGCTCCG1970.30528909482558225No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGAGTACTTAACACTTTTGCTTCG1880.29134187729547956No Hit
CAGCCTTGCGACCATACTCCCCCCAGAACCCAAAGACTTTGATTTCTCAT1770.27429527809202064No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG1690.261897751398596No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGAGCTTAATGCGTTAGCTGCG1670.2587983697252398No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTTTG1650.2556989880518837No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1620.2510499155418494No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTACTTAATGCGTTAGCTACG1610.24950022470517133No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1540.23865238884842474No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGCG1500.2324536255017124No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTATCGCGTTAGCTTCG1270.1968107362581165No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTCGCTGCG1230.19061197291140416No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG1190.18441320956469184No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGATTACTTAACGCTTTCGCTTAG1180.18286351872801376No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGGGTACTTAACACTTTTGCTTCG1140.1766647553813014No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTACG1130.17511506454462336No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGAGAACTTATCGCGTTAGCTTCG1130.17511506454462336No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTTAAAGCGTTAGCTGCG1110.17201568287126717No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCG1100.1704659920345891No Hit
CAGTCTTGCGACCATACTCCCCCCAGAACCCAAAGACTTTGATTTCTCAT1090.168916301197911No Hit
TAATCTTGCGATCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG1060.16426722868787677No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGTTTAATGCGTTAGCTGCG1040.1611678470145206No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT1010.15651877450448634No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG990.15341939283113018No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG940.14567093864773978No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTTAAAGCGTTAACTGCG930.1441212478110617No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCTACTTAGTGCGTTAGCTGCG930.1441212478110617No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAGAACTTAGCGCGTTAGCTTCG920.1425715569743836No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATTACTTAATGCGTTAGCTGCG900.13947217530102743No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTATCGCGTTTGCTGCG900.13947217530102743No Hit
CAATCTTGCGATCGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG880.13637279362767127No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTACTGCGTTAGCTGCG880.13637279362767127No Hit
TAATCTTGCGACCGTAGTCCCCAGGCGAAGTGCTATTCACGTTAGCTATG870.13482310279099322No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGCGCTTACCGCGTTGGCTACG860.1332734119543151No Hit
CAACCTTGCGGCCGTACTTCCCAGGCGGAAGACTTAACGCGTTAGCTAAT850.13172372111763703No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG840.13017403028095895No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG820.12707464860760279No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGAACACTTAACGCTTTCGCTACG820.12707464860760279No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG820.12707464860760279No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCTACTTAGTGCGTTAGCTGCG810.1255249577709247No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG810.1255249577709247No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTACCGCGTTAGCTACG810.1255249577709247No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCT810.1255249577709247No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTTCG800.12397526693424663No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTAATGCGTTAGCTGCG780.12087588526089046No Hit
AAGCCTTGCGACCATACTCCCCAGGCGGGGCACTTCACGGTTTCCCTTCG780.12087588526089046No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATATCTTAATGCGTTAGCTGCG770.11932619442421237No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGCG760.11777650358753429No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG740.11467712191417813No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTTAACTTAATGCGTTAGCTGCG740.11467712191417813No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG730.11312743107750003No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGCTCACTTACCGGTTTCCCTACG730.11312743107750003No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTGCG710.11002804940414387No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG710.11002804940414387No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG670.10382928605743155No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTCATGCGTTAGCTGCG660.10227959522075346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCATG100.0013653996251.280272
TCGGCCG100.0013653996251.280278
AACATTG100.0013653996251.280272
ATGCGGC100.0013653996251.280276
GTGAGAC100.0013653996251.280276
ATAGTGA100.0013653996251.280273
AATCGTG400.0251.280272
CGAACGT251.371518E-9251.280269
GCGAACG251.371518E-9251.280268
ATTGCGG251.371518E-9251.280265
ATTGCGA500.0251.280265
CATGCGA450.0251.280245
TTGCGAT1400.0251.280246
GCGATCG1400.0251.280248
GTGCGGC151.36725575E-5251.280246
GCGACCG33600.0250.158468
CGACCGT33050.0250.139799
TGCGACC40250.0250.031657
GCCTTGC8250.0249.757353
GCGACCA6700.0249.405038
Produced by FastQC (version 0.11.5)