FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61490
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1032516.791348186697025No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG42506.911692958204586No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG26234.265734265734266No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATTATTTAATGCGTTAGCTGCG23063.750203285087006No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG9251.5043096438445276No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG7421.2067002764677184No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG6431.0456984875589528No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG6211.0099203122458937No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG5970.9708895755407383No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTTAG4990.8115140673280208No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGTTTAATGCGTTAGCTGCG4600.7480891201821435No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG3440.5594405594405595No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGCACACTTAACGCGTTAGCTCCG3400.5529354366563668No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGGGCACTTCACGGTTTCCCTGCG3370.5480565945682225No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG3260.530167506911693No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG3200.5204098227354041No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG3150.5122784192551635No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTAACGCGTTAGCTGCG2980.4846316474223451No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGAAGCTTAATGCGTTAGCTGCG2970.48300536672629696No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTCATAGGGTTACCTGCG2490.40494389331598635No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGGGCACTTCACGGTTTCCCTTCG2440.39681248983574563No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGATAACTTAATGCGTTAGCTGCG2410.39193364774760125No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG2350.3821759635713124No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT2260.36753943730687916No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG2260.36753943730687916No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG2240.36428687591478287No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTACCGCGTTAGCTACG2220.36103431452268664No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG2110.3431452268661571No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1910.31061961294519436No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGCGCTTACCGCGTTGGCTACG1890.30736705155309807No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTATCGCGTTAGCTACG1820.2959830866807611No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATTGCTTAATGCGTTAGCTGCG1670.271588876240039No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATTACTTAATGCGTTAGCTGCG1650.26833631484794274No Hit
TAATCTTGCGATCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG1620.26345747275979836No Hit
CAATCTTGCGACCGTACTCCCCAGGTGGCATACTTAATGCTTTCGCTTAG1620.26345747275979836No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTCGCTGCG1570.25532606927955764No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG1550.2520735078874614No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG1520.24719466579931695No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGCG1490.24231582371117255No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG1470.23906326231907626No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTACTGCGTTAGCTGCG1440.23418442023093186No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1410.22930557814278743No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTTCG1310.21304277118230608No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG1220.19840624491787281No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGAGCTTAATGCGTTAGCTGCG1190.1935274028297284No Hit
CAGCCTTGCGACCATACTCCCCCCAGAACCCAAAGACTTTGATTTCTCAT1150.18702228004553587No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTATCAT1090.17726459586924703No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATATCTTAATGCGTTAGCTGCG1010.16425435030086194No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1000.1626280696048138No Hit
TAATCTTGCGACCGTAGTCCCCAGGCGAAGTGCTATTCACGTTAGCTATG970.15774922751666937No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCT960.15612294682062125No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAAGACTTAATGCGTTAGCTGCG950.1544966661245731No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTAATGCGTTAGCTGCG900.14636526264433242No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGCG870.14148642055618801No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAGAACTTATCGCGTTAGCTACG860.13986013986013987No Hit
TAATCTTGCGACCGTACTCCCCATGCGGATAACTTAATGCGTTAGCTGCG850.1382338591640917No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG850.1382338591640917No Hit
TAATCTTGCGACCGTACTCCCCAGGTGGATAACTTAATGCGTTAGCTGCG810.13172873637989918No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG810.13172873637989918No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTTAAAGCGTTAGCTGCG800.13010245568385104No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGTGCTTAATGTGTTAACTTCG790.1284761749878029No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATGCGTTAGCTGCG780.12684989429175475No Hit
CAGTCTTGCGACCATACTCCCCCCAGAACCCAAAGACTTTGATTTCTCAT780.12684989429175475No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG770.12522361359570663No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG760.12359733289965848No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTCATGCGTTAGCTGCG760.12359733289965848No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTGCG750.12197105220361035No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGTACTTAATGCGTTAGCTACG740.1203447715075622No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTATCGCGTTAACTTCG730.11871849081151406No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG730.11871849081151406No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTCGCTTCG720.11709221011546593No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTTAACTTAATGCGTTAGCTGCG710.11546592941941779No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG710.11546592941941779No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTATTGCGTTAGCTGCG690.11221336802732151No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG690.11221336802732151No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTTAAAGCGTTAACTGCG680.11058708733127338No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG670.10896080663522524No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGAGTACTTAACACTTTTGCTTCG660.1073345259391771No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGCGCTTATCGCGTTGGCTACG640.10408196454708082No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTTCG640.10408196454708082No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG630.1024556838510327No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTACG630.1024556838510327No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATTATTTAATGCGTTAGCTGCG620.10082940315498455No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTATCGCGTTAGCTTCG620.10082940315498455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG100.0013600324251.600855
GCGAACG450.0251.600858
CATGAGG100.0013600324251.600855
CGACCGA201.3570934E-7251.600859
GCCGGCG100.0013600324251.600858
CGCGACC201.3570934E-7251.600857
TGCGGCC12850.0251.600857
TTGAGAC350.0251.600856
AGGCCGG100.0013600324251.600859
AATCTCG201.3570934E-7251.600852
TTGCGAT850.0251.600856
ATGCGAC500.0251.600856
TTGCCGG100.0013600324251.600856
ACCGTGC100.0013600324251.600853
AATAGTG100.0013600324251.600852
TCGACCG100.0013600324251.600858
GTGCGGC100.0013600324251.600856
GTGCGAA100.0013600324251.600856
CGTGCGA650.0251.600835
AGTGCGA151.3599869E-5251.600835
Produced by FastQC (version 0.11.5)