FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n01_EMP_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n01_EMP_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339420
Sequences flagged as poor quality0
Sequence length251
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA13319539.24194213658594No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA3456710.184137646573568No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA145834.296446879971716No Hit
TTCCAGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTA73862.1760650521477816No Hit
TACGGGGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCATGTA66911.9713039891579753No Hit
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA65421.9274055742148373No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA57721.700547993636203No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA53021.5620764834128809No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA47271.3926698485651994No Hit
TACGTAGGGTGCGAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA43301.27570561546167No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA38121.1230923339814978No Hit
TACAGGGGATGCAAGTGTTATCCGGAATTATTGGGCGTAAAGGGTCTCTA32400.9545695598373696No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCGTGTA23030.6785104000942785No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA20690.6095692652171352No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA20020.5898297095044487No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA19990.5889458488008956No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA19930.5871781273937895No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA14680.4325025042719934No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA12730.3750515585410406No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA12290.3620882682222615No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA12120.35707972423546047No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGCGCACGCA11930.35148193977962405No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA11520.3394025101643981No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA11310.33321548523952627No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA11050.3255553591420659No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10730.3161275116374993No Hit
TACGTAGGGTGCAAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10690.3149490306994284No Hit
TACGTAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGCA9820.2893170702963879No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA9080.26751517294207766No Hit
TACGAAGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGCGTA8810.2595604266100996No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA7760.22862530198574038No Hit
TACAGAGGTGGCGAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA7180.21153732838371336No Hit
TACGGAGGGGGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA7130.21006422721112486No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA6810.20063637970655826No Hit
TACGAAGGGAGTGAGCGTTATTCATAATGATTGGGCGTAAAGGGTTCGTA6630.19533321548523952No Hit
TACGTAGGTTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA6400.18855695009133228No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA5920.17441517883448235No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA5150.15172942077661894No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAAATACTGGGCGTAAAGCGTACGTA4630.1364091685816982No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA4480.1319898650639326No Hit
TACGGAGGGAGCTAGCGNTGTTCGGAATTACTGGGCGTAAAGCGCACGTA4370.12874904248423782No Hit
TACATAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA4260.12550821990454303No Hit
TACGTAGGGAGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA4150.12226739732484827No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA4080.12020505568322432No Hit
TACGTAGGGTGCTAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA3960.11666961286901185No Hit
TCCGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA3930.11578575216545871No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA3840.11313417005479937No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA3730.10989334747510458No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGAGCGTA3710.10930410700606917No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA3650.10753638559896295No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3610.10635790466089212No Hit
TACGTAGGGTGCGAGCGTTAATCGGCATTACTGGGCGTAAAGCGTGCGCA3500.10311708208119734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGAGA400.0244.795822
CCCCAAG100.0014794329244.795829
ACGTATG900.0244.795822
TCGACCA100.0014794329244.79582245
GCCAACC251.6207196E-9244.79582245
GGTCCAA350.0244.79588
TAGGGCG301.6370905E-11244.79585
GGTTGCA1050.0244.79587
GTCCAAG350.0244.79589
CAGAGAC301.6370905E-11244.79583
GTAGGGT195300.0244.670464
TAGGGTG195600.0244.545525
GTGCGAG165550.0244.426159
AGGATCC21250.0244.219826
GAGGATC20700.0244.204515
ATCCAAG15050.0243.982549
CGTAGGG198900.0243.81123
TTCCAGC9900.0243.559481
ACGGAGG65000.0243.477682
TACGGAG65200.0243.293991
Produced by FastQC (version 0.11.5)