FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n01_EMP_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n01_EMP_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83182
Sequences flagged as poor quality0
Sequence length251
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA1059712.73953499555192No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA963011.577023875357648No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA61617.406650477266716No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA44595.360534731071627No Hit
TACGAAGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGCGTA37764.5394436296314105No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA31473.7832704190810515No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA25233.0331081243538267No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA20082.4139837945709406No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA15671.8838210189704503No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA14081.6926738957947634No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGCA13531.6265538217402804No Hit
TACGGGGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCATGTA11161.3416364117236903No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA10661.281527253492342No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA8901.0699430165179966No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA8371.0062273087927678No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA8180.9833858286648554No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA6630.7970474381476762No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA6460.7766103243490179No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA5420.6515832752278138No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA5110.6143155971243779No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA4360.5241518597773557No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGAGCGTA3860.4640427015460076No Hit
TACATAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA3670.44120122141809526No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGCGCACGCA3380.40633790964391336No Hit
TACGTAGGGTGCGAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA3110.37387896419898536No Hit
TACGGAGGATCCAAGCGNTATCCGGAATCATTGGGTTTAAAGGGTCCGTA3070.36907023154047747No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA2960.35584621672958094No Hit
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGCTTAAAGGGTCCGTA2410.289726142675098No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA2370.2849174100165901No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTACGTA2170.2608737467240509No Hit
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGCA1840.22120170229136113No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCGTGTA1840.22120170229136113No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA1820.21879733596210718No Hit
TACGTAGGGTGCAAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1710.2055733211512106No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA1690.20316895482195668No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA1690.20316895482195668No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGCA1680.2019667716573297No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA1670.20076458849270276No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1610.19355148950494097No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA1570.18874275684643313No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA1530.1839340241879253No Hit
TACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA1510.18152965785867137No Hit
TACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA1470.1767209252001635No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1450.17431655887090958No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1420.17071000937702868No Hit
TACAGAGGGTGCGAGCGNTAATCGGATTTACTGGGCGTAAAGCGTGCGTA1370.16469909355389387No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1360.1634969103892669No Hit
TACGAAGGATCCAAGCGTTGTCCGGATTTACTGGGTTTAAAGGGTGCGTA1310.1574859945661321No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1290.15508162823687818No Hit
TACGGAGGATCCAAGCGTTATCCGGAATTATTGGGTTTAAAGGGTCCGTA1280.1538794450722512No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGAGCGTA1210.14546416291986247No Hit
TACGAAGGGTGCAAGCGNTGTTCGGATTTATTGGGCGTAAAGCGCGCGTA1120.1346445144382198No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1060.12743141545045802No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA1050.12622923228583108No Hit
TACGGAGGGTGCAAGCGNTATCCGGATTTATTGGGTTTAAAGGGTCCGTA1050.12622923228583108No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGCA1040.1250270491212041No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA1040.1250270491212041No Hit
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTGCGTA990.11901613329806929No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA980.11781395013344233No Hit
TACGTAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA980.11781395013344233No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA950.11420740063956143No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGTGTA940.11300521747493449No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA920.11060085114568056No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA920.11060085114568056No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA910.10939866798105359No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA910.10939866798105359No Hit
TACGTAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGCA880.10579211848717271No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA870.10458993532254575No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA850.10218556899329181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATAG900.0244.80641
ACATAGG900.0244.80642
CAAACCG100.0014772398244.80638245
ACAAACA100.0014772398244.80638245
CGAACGG400.0244.80638245
CGAACAG100.0014772398244.80638245
GCGAAAA100.0014772398244.80638245
TCACAAG201.5592559E-7244.806389
GCGCGAG100.0014772398244.806389
TGGGTGC100.0014772398244.806386
GGTCCCG100.0014772398244.806387
GGTCCAG251.6079866E-9244.806388
GCAAAAG100.0014772398244.80638245
GGATCCG3500.0244.806387
TACGGTG100.0014772398244.806381
TAGGGTG22050.0244.806385
TAGGGCG100.0014772398244.806385
GGTGCAT100.0014772398244.806388
TCCAACA100.0014772398244.80638245
ACGTATG251.6079866E-9244.806382
Produced by FastQC (version 0.11.5)