FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n01_EMP_36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n01_EMP_36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80864
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA2742033.90878512069648No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA859810.632667194301543No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA73169.04728927582113No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA21672.679808072813613No Hit
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA17042.107241788682232No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA16432.031806489908983No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA13181.6298971111990503No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA13161.6274238227146816No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA13131.6237138899881283No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA12361.5284922833399288No Hit
TACGAAGGGTGCAAGCGNTAATCGGAATTACTGGGCGTAAAGCGCGCGTA8911.101850019786308No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA8531.0548575385833003No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA7640.944796201028888No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA7000.8656509695290859No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA6750.8347348634744756No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA5550.6863375544123467No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA4420.5465967550455084No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA4030.49836762960031655No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA3860.47734467748318166No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA3770.46621487930352196No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA3620.44766521567075584No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA3100.3833597150771666No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA3040.37593984962406013No Hit
TACGTAGGGTGCGAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2980.36851998417095366No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2460.30421448357736447No Hit
TACGTAGGGTGCAAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2390.2955579738820736No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA2290.2831915314602295No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA2290.2831915314602295No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA2280.28195488721804507No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA2130.263405223585279No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA2100.2596952908587258No Hit
GACGAGGGATCCTAGCGTTGTTCGGAATCATTGGGCGTAAAGCGGGTGTA1910.236199050257222No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA1830.2263058963197467No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1820.22506925207756234No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA1740.21517609814008706No Hit
TACGGGGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCATGTA1590.19662643450732092No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA1510.18673328056984567No Hit
TACGAAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1440.1780767708745548No Hit
TACGGAGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA1400.17313019390581716No Hit
TACGGAGGGTCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1340.16571032845271072No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1300.1607637514839731No Hit
TACGAAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA1210.14963395330431342No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1190.1471606648199446No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGCA1060.13108428967154728No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA1060.13108428967154728No Hit
TACATAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA1030.12737435694499408No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA1030.12737435694499408No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGTGCGTAAAGCGCGCGTA970.11995449149188762No Hit
TACGAAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA950.1174812030075188No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA910.11253462603878117No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA890.11006133755441234No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA850.1051147605856747No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCACGCA820.10140482785912149No Hit
TACGTAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGCA820.10140482785912149No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA810.10016818361693709No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA810.10016818361693709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTCT100.0014774202244.79225
AGGATGC1200.0244.79226
AGGATCC4550.0244.79226
GTCCGAG301.6370905E-11244.79229
TCGAACA550.0244.7922245
GGATCCT500.0244.79227
ATGCGAG550.0244.79229
CGAGGGA600.0244.79223
GCAAAAC100.0014774202244.7922245
GATCCTA500.0244.79228
AGTGTGC100.0014774202244.79226
TGAAGGG100.0014774202244.79223
AGGTCTC100.0014774202244.79226
GACGAGG550.0244.79221
GTAGGGC100.0014774202244.79224
GGGGCTA1900.0244.79228
GCCAACC100.0014774202244.7922245
TAGGGCG100.0014774202244.79225
GGGCGAG151.5193022E-5244.79229
TCCGAAG151.5193022E-5244.79221
Produced by FastQC (version 0.11.5)