FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n01_EMP_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n01_EMP_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74392
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2800337.64248843961716No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA798810.737713732659426No Hit
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA36034.843262716421121No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA34534.64162813205721No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA29663.986987848155716No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGCA19522.6239380578556832No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA16902.2717496505000536No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA13211.7757285729648349No Hit
TACGTAGGGTGCGAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA8981.2071190450586085No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA7060.9490267770728036No Hit
TACGAAGGATCCAAGCGTTGTCCGGATTTACTGGGTTTAAAGGGTGCGTA6170.8293902570168834No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA5950.79981718464351No Hit
TACGGGGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCATGTA4280.5753306807183568No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA4270.5739864501559307No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA3630.4879556941606624No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3620.48661146359823637No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA3260.43821916335089794No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2530.34009033229379504No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA2480.3333691794816647No Hit
TACGTAGGGTGCAAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2320.3118614904828476No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2310.31051725992042156No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2150.2890095709216045No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA1950.26212495967308314No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA1900.2554038068609528No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGAGCGTA1450.19491343155177976No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA1430.19222497042692763No Hit
TACGGAGGATCCAAGCGNTATCCGGAATCATTGGGTTTAAAGGGTCCGTA1420.19088073986450158No Hit
TACGTAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGCA1390.18684804817722334No Hit
TACGAAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1290.1734057425529627No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGAGCGTA1150.15458651467899776No Hit
TACGGAGGGAGCTAGCGNTGTTCGGAATTACTGGGCGTAAAGCGCACGTA1030.13845574792988494No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTA1010.13576728680503278No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATCACTGGGCGTAAAGGGTGCGTA1000.13442305624260673No Hit
TACGGAGGGTGCAAGCGNTATCCGGATTTATTGGGTTTAAAGGGTCCGTA980.1317345951177546No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA960.12904613399290246No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA930.12501344230562425No Hit
TCCGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA920.12366921174319818No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA870.11694805893106784No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGCA860.1156038283686418No Hit
TTCCAGCTCCAAGAGTGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTA840.11291536724378966No Hit
TACGTAGAGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA830.11157113668136358No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGCTTAAAGGGTCCGTA790.10619421443165931No Hit
TACCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA770.1035057533068072No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA750.10081729218195505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACA201.5587284E-7244.78818245
TAGTGTG100.0014772547244.788185
AGGATCC5550.0244.788186
ACGTATG100.0014772547244.788182
GGACACA100.0014772547244.78818245
TACGTAT100.0014772547244.788181
CGAACAG100.0014772547244.78818245
GAATCCA100.0014772547244.788187
TGGTGCG100.0014772547244.788187
CAAATAG800.0244.78818245
AGCTCCA1950.0244.788165
TCGAACA251.6079866E-9244.78816245
ATGGTGC151.518912E-5244.788166
GGATCCG251.6079866E-9244.788167
TACATAG251.6079866E-9244.788161
TCAAACA2250.0244.78816245
ACATAGG251.6079866E-9244.788162
GATCCGA251.6079866E-9244.788168
GATCCAA5300.0244.788168
TTCCAGC1800.0244.788161
Produced by FastQC (version 0.11.5)