FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n01_EMP_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n01_EMP_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101908
Sequences flagged as poor quality0
Sequence length251
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA6221861.05310672371158No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA27912.738744750166817No Hit
TACAGAGGGTGCGAGCGNTAATCGGATTTACTGGGCGTAAAGCGTGCGTA22712.228480590336382No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA19961.9586293519645173No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA14221.395376221690152No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10681.0480040821132786No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGCA9830.9645955175256113No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA8590.8429171409506614No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCACGCA6920.6790438434666561No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA6460.6339050908662716No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA5110.501432664756447No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA4390.4307807041645405No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCGTGTA4270.4190053773992228No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA3930.3856419515641559No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCGCGTA3770.3699415158770656No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA3040.298308278054716No Hit
TACGTAGGGAGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGCGTGTA2700.2649448522196491No Hit
TACAGCGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA2390.2345252580759116No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA2280.223731208541037No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA2190.21489971346704873No Hit
TACGGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA2030.1991992777799584No Hit
TACATAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA1970.19331161439729955No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCATGTA1940.1903677827059701No Hit
TACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA1870.1834988420928681No Hit
TACGAAGGGGGCAAGCGTTATTCGGAATGACTGGGCGTAAAGGGCGTGTA1780.17466734701887976No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTACGTA1630.15994818856223258No Hit
TCCAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA1480.14522903010558544No Hit
TACAGAGGGAGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA1450.142285198414256No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1420.13934136672292655No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA1420.13934136672292655No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGCA1410.1383600894924834No Hit
TACAGAGGGTGCGAGCGTTAATCGGCTTTACTGGGCGTAAAGCGTGCGTA1340.1314911488793814No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA1200.11775326765317738No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGTA1130.11088432704007536No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1110.10892177257918907No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA1030.10107155473564391No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA1030.10107155473564391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGT550.0244.769884
GGTCCGG900.0244.769888
CCAATAG350.0244.769889
CCAGCTC450.0244.769883
CTCCAAT350.0244.769887
TACATAG550.0244.769881
CATAGGG550.0244.769883
CAGCTCC450.0244.769884
AGCTCCA450.0244.769885
ACATAGG550.0244.769882
GCTCCAA450.0244.769886
TCAAACA900.0244.76988245
CGGGTGC151.5210149E-5244.769876
CAGCGGG151.5210149E-5244.769873
CAAAAAA151.5210149E-5244.76987245
GGATGCA151.5210149E-5244.769877
GGATCCG950.0244.769877
GTAGGGT4500.0244.769874
GAGCGAG151.5210149E-5244.769879
GAAACAG151.5210149E-5244.76987245
Produced by FastQC (version 0.11.5)