FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n01_EMP_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n01_EMP_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90247
Sequences flagged as poor quality0
Sequence length251
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA997411.051890921581881No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA81639.045176016931311No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA78388.685053242767072No Hit
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA72428.024643478453578No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA55986.202976276219708No Hit
TACGGGGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCATGTA49225.453920905958093No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA33973.7641140425720523No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA26692.957439028444159No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA19962.211707868405598No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA17171.902556317661529No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA11531.2776047957272818No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA11041.2233093620840583No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA8860.9817500858754307No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA8820.9773178055780247No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA7910.8764834288120381No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGCA7290.807783084202245No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA6960.7712167717486453No Hit
TACGTAGGTTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA6940.7690006315999424No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCGTGTA6750.7479473001872639No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA6300.6980841468414463No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA5890.6526532737930347No Hit
TACGAAGGGGGCAAGCGTTATTCGGAATGACTGGGCGTAAAGGGCGTGTA4270.4731459217480914No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA4130.4576329407071703No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGCGCACGCA3690.40887785743570426No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATCACTGGGCGTAAAGGGAGCGTA3400.37674382527951067No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATCACTGGGCGTAAAGGGTGCGTA3260.36123084423858964No Hit
TACGTAGGGTGCGAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA3260.36123084423858964No Hit
TACGGAGGGTGCAAGCGNTATCCGGATTTATTGGGTTTAAAGGGTCCGTA2820.31247576096712354No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA2810.31136769089277205No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2750.3047192704466631No Hit
TACGGAGGATCCGAGCGNTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2590.28699014925703903No Hit
TACGGAGGATCCAAGCGNTATCCGGAATCATTGGGTTTAAAGGGTCCGTA2410.267044887918712No Hit
TACGAAGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGCGTA2240.24820769665473644No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA2110.23380278568816693No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGTGCGTA2070.22937050539076093No Hit
GACGAGGGATCCTAGCGTTGTTCGGAATCATTGGGCGTAAAGCGGGTGTA2060.22826243531640938No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTACGTA2020.22383015501900339No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGCTTAAAGGGTCCGTA1990.2205059447959489No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA1860.2061010338293794No Hit
TACGTAGGGTGCAAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1820.20166875353197336No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA1810.20056068345762185No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1750.19391226301151285No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1670.18504770241670082No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1600.17729121189624034No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1440.15956209070661628No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1440.15956209070661628No Hit
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGCTTAAAGGGTCCGTA1430.1584540206322648No Hit
TACGGGGGGTGCAAGCGNTACTCGGAATCACTGGGCGTAAAGAGCATGTA1430.1584540206322648No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGATGCGTA1420.15734595055791328No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA1400.15512981040921028No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA1290.14294103959134374No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1290.14294103959134374No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA1250.13850875929393774No Hit
TACGTAGGGAGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGCGTGTA1150.12742805855042275No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA1150.12742805855042275No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGCA1140.12631998847607123No Hit
TACGAAGGGTGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGCGCGCGCA1140.12631998847607123No Hit
TACGGAGGGTGCAAGCGNTAATCGGAATTACTGGGCGTAAAGCGCACGCA1130.12521191840171972No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA1110.12299577825301672No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGAGCGTA1090.1207796381043137No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA1050.11634735780690772No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1000.1108070074351502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAATAG3050.0244.79048245
AGGATCC22300.0244.790486
GAGGATC22150.0244.790475
CGAACGG100.0014777342244.79047245
CGAACAG400.0244.79047245
GGGTCCA201.5604928E-7244.790477
TCACAAG100.0014777342244.790479
GCAACCA800.0244.79047245
TAGGTCC100.0014777342244.790475
GACGTAG100.0014777342244.790471
GATCCTA650.0244.790478
GTCTCAA151.5199268E-5244.790478
GGTCCAG301.6370905E-11244.790478
GGTCCAA201.5604928E-7244.790478
GGATCCA11700.0244.790477
TACGGGG6200.0244.790471
GAGGTCT100.0014777342244.790475
CGTAGGA151.5199268E-5244.790473
GAAACAG151.5199268E-5244.79047245
GGAACCA100.0014777342244.790477
Produced by FastQC (version 0.11.5)