FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n01_EMP_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n01_EMP_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62331
Sequences flagged as poor quality0
Sequence length251
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA3380454.23304615680801No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA47887.681570967897194No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA36005.775617269095635No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA12001.9252057563652114No Hit
TACGTAGGGTGCGAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10791.731080842598386No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA7141.145497425037301No Hit
TACGAAGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGCGTA5270.8454861946703888No Hit
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA4950.7941473745006496No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA4590.7363912018096934No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA3770.6048354751247373No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA3440.551892316824694No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA2610.4187322520094335No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA2470.3962715181851727No Hit
TACGTAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGCA2420.388249827533651No Hit
TTCCAGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTA2170.3481413742760424No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2040.327284978582086No Hit
TACGTAGTGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1690.27113314402143396No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1480.23744204328504276No Hit
TACGTAGGGTGCAAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1390.22300300011230367No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1330.21337697133047762No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGAGCGTA1300.2085639569395646No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA1260.2021466044183472No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA1160.18610322311530378No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA1130.18129020872439075No Hit
TACGGAGGGAGCTAGCGNTGTTCGGAATTACTGGGCGTAAAGCGCACGTA1090.1748728562031734No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1070.1716641799425647No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA1060.17005984181226036No Hit
TACGTAGGGTGCGAGCGTTAATCGGCATTACTGGGCGTAAAGCGTGCGCA990.15882947490012994No Hit
TACGTAGGGTGCGAGCGTTAATCGGAGTTACTGGGCGTAAAGCGTGCGCA960.1540164605092169No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA860.1379730792061735No Hit
TACGTAAGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA860.1379730792061735No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAGGCGTGCGCA790.12674271229404308No Hit
TACGTAGGGGGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA770.12353403603343441No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGTGCGTA760.12192969790313006No Hit
TACGTAGGGAGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA760.12192969790313006No Hit
TACGTATGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA750.12032535977282571No Hit
TACGTAGGGTGCGCGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA720.11551234538191268No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTGCGCA680.10909499286069532No Hit
TCCGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA670.10749065473039097No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA660.10588631660008664No Hit
TACGGGGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCATGTA660.10588631660008664No Hit
TACGAAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA640.10267764033947795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACA301.6370905E-11244.79776245
GGATGCA100.0014765082244.797767
GAGCGAG301.6370905E-11244.797769
CCAGCTC301.6370905E-11244.797763
ATCCAAG600.0244.797769
AGGATGC151.5174954E-5244.797766
ATAGGGT151.5174954E-5244.797764
TCGAACA151.5174954E-5244.79776245
GGATCCG100.0014765082244.797767
TCCAGCT301.6370905E-11244.797762
GCAATCA151.5174954E-5244.79776245
TACATAG100.0014765082244.797761
TCAAACA800.0244.79776245
TACGTAT151.5174954E-5244.797761
ACATAGG100.0014765082244.797762
GCAAACC1100.0244.79776245
GATCCGA100.0014765082244.797768
GATCCAA600.0244.797768
TTCCAGC301.6370905E-11244.797761
AAGGTGC100.0014765082244.797766
Produced by FastQC (version 0.11.5)