FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n01_EMP_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n01_EMP_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112227
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2093318.65237420585064No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1393612.417689147887764No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA1355812.08087180446773No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA71236.346957505769556No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA55604.954244522262914No Hit
TACGGGGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCATGTA37463.337877694315985No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA30812.745328664225186No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA19571.7437871456957774No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA15841.411425058141089No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA14361.2795494845268964No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA10530.9382768852415193No Hit
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA9110.81174761866574No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAAATACTGGGCGTAAAGCGCACGTA8790.7832339811275362No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA7930.7066035802436134No Hit
TACGTAGGGTGCGAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA7050.6281910770135529No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGAGCGTA7040.6273000258404839No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA6370.5675995972448697No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA5890.524829140937564No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA5670.5052260151300489No Hit
TACGGAGGGGGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA5620.5007707592647046No Hit
TACGTAGGGTGCAAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA4240.3778056973812006No Hit
TACGGAGGGAGCTAGCGNTGTTCGGAATTACTGGGCGTAAAGCGCACGTA4190.3733504415158562No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA4170.3715683391697185No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA3250.2895916312473826No Hit
TACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA2840.2530585331515589No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA2460.21919858857494187No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGCA2430.21652543505573524No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA2430.21652543505573524No Hit
TACGGAGGGTGCAAGCGNTATCCGGATTTATTGGGTTTAAAGGGTCCGTA2270.20226861628663334No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA2260.20137756511356447No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGCA2210.1969223092482201No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA2100.18712074634446257No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGCA2020.1799923369599116No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGCTTAAAGGGTCCGTA2010.17910128578684273No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA1880.16751762053694744No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTA1820.16217131349853423No Hit
TACGAAGGGGGCTAGCGTTACTCGGAATTACTGGGCGTAAAGGGCGCGTA1790.15949815997932762No Hit
TACAGAGGGTGCGAGCGNTAATCGGATTTACTGGGCGTAAAGCGTGCGTA1770.15771605763318988No Hit
TACGTAGGGAGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGCGTGTA1680.14969659707557004No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAAATACTGGGCGTAAAGCGTACGTA1660.1479144947294323No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA1620.14435029003715683No Hit
TACGTAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA1580.14078608534488135No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1580.14078608534488135No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA1570.13989503417181248No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCACGCA1540.13722188065260588No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGTGCGTA1500.1336576759603304No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1460.13009347126805493No Hit
TACGTAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA1460.13009347126805493No Hit
TACGTAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGCA1450.12920242009498606No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA1430.12742031774884832No Hit
TACGGGGGGTGCAAGCGNTACTCGGAATCACTGGGCGTAAAGAGCATGTA1420.12652926657577945No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA1380.12296506188350398No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTGCGCA1220.10870824311440205No Hit
TACGTAGGGGGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1190.10603508959519546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACA151.5208998E-5244.79102245
ACGGCGG301.6370905E-11244.791022
ATGGTGC151.5208998E-5244.791026
TAGTGTG151.5208998E-5244.791025
GACGAAG151.5208998E-5244.791021
TCAAACA1550.0244.79102245
CGAACAG100.001478204244.791245
GCGAACG400.0244.791245
GACGTAG100.001478204244.7911
GATCCAA1100.0244.7918
GGTCCCG100.001478204244.7917
GTAGGGT44250.0244.7914
CAGGGTG100.001478204244.7915
TCCAGAG100.001478204244.7911
GAGCGAG500.0244.7919
GTCCGAG201.5621663E-7244.7919
TAGGGCG201.5621663E-7244.7915
GCAGGGT100.001478204244.7914
GGTTGCA100.001478204244.7917
ACGTATG100.001478204244.7912
Produced by FastQC (version 0.11.5)