FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n01_EMP_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n01_EMP_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66284
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2352735.49423691992034No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA897313.53720354836763No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA57478.67026733450003No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA33004.978577032164625No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA27984.221229859392915No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA12951.9537143201979361No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA10751.6218091847202944No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGAGCGTA9771.4739605334620722No Hit
TACGTAGGGTGCGAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA8071.2174883833202583No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA5240.7905376863194739No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA4260.6426890350612515No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA2830.42695069700078453No Hit
TACGTAGGGTGCAAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2620.3952688431597369No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA2480.3741476072657052No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA2370.35755235049182305No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA2060.3107838995836099No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2030.3062579204634603No Hit
TACGGAGGGAGCTAGCGNTGTTCGGAATTACTGGGCGTAAAGCGCACGTA1930.29117132339629476No Hit
TACGGAGGGGGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA1850.2791020457425623No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA1740.26250678896868024No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1660.2504375113149478No Hit
TACGTAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGCA1580.23836823366121537No Hit
TACGGAGGGTGCAAGCGNTATCCGGATTTATTGGGTTTAAAGGGTCCGTA1330.20065174099330157No Hit
TACGGAGGGAGCTAGCGNTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA1250.18858246333956913No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGCTTAAAGGGTCCGTA1150.1734958662724036No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA1130.1704785468589705No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA1080.16293524832538772No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTGCGCA1040.15690060949852153No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1020.15388329008508841No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA990.14935731096493873No Hit
TACGTAGGGAGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGCGTGTA980.1478486512582222No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGCA950.14332267213807254No Hit
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA870.13125339448434012No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA860.12974473477762355No Hit
TACGTAGGGTGCGAGCGTTAATCGGCATTACTGGGCGTAAAGCGTGCGCA820.12371009595075735No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA820.12371009595075735No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGCA820.12371009595075735No Hit
TACGGAGGGTGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA740.11164081829702491No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA690.10409751976344216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGTGC151.5184152E-5244.778126
GGATCCA151.5184152E-5244.778127
AGTGTGC151.5184152E-5244.778126
AAGGTGC151.5184152E-5244.778126
GTAGGGT38700.0244.778124
TAAGGTG151.5184152E-5244.778125
TACAGAG1700.0244.77811
GCAGGGT100.0014770738244.77814
ACGTATG100.0014770738244.77812
ATGCGAG201.5577643E-7244.77819
CGGCGGG100.0014770738244.77813
CAGGGTG100.0014770738244.77815
TACGTAT100.0014770738244.77811
GATCCGA100.0014770738244.77818
CGAACAG100.0014770738244.7781245
GGGGCTA3650.0244.77818
GTGGCAA201.5577643E-7244.77818
TAGGGGG201.5577643E-7244.77815
GCCAACC100.0014770738244.7781245
GGGCGCT100.0014770738244.77817
Produced by FastQC (version 0.11.5)