FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n01_EMP_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n01_EMP_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63436
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA51278.082161548647456No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCACGCA45117.111104104924648No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA40266.346554007188347No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA35555.604073396809383No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA34775.481114824389937No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA30854.8631691783845135No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA20393.214263194400656No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA15832.495428463333123No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCGTGTA12441.96103159089476No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA10721.68989217479034No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA9441.48811400466612No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA9341.4723500851251654No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA7671.2090926287912227No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA7611.1996342770666497No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA7421.169682829938836No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA6831.0766757046472035No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA6771.0672173529226308No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA6441.0151964184374802No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGATGCGTA5130.8086890724509741No Hit
TACGTAGGGAGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGCGTGTA4900.7724320575067785No Hit
TACGGAGGGTGCAAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA4570.7204111230216281No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCGCGTA3980.6274039977299956No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGATGCGTA3930.6195220379595183No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA3410.537549656346554No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCATGTA3340.5265149126678857No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGCA2830.446118923009017No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2600.40986190806482126No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGCGCACGCA2340.3688757172583391No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCTCGTA2300.36257014944195726No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGTGCGTA2240.35311179771738443No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2130.3357714862223343No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA2080.327889526451857No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1870.2947852954158522No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA1850.2916325115076613No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCGCGTA1840.2900561195535658No Hit
TACGTAGGTGGCAAGCGNTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1640.25852828047165644No Hit
TACGTAGGGGGCAAGCGNTGTCCGGAATTACTGGGCGTAAAGCGCACGCA1570.2474935367929882No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1540.2427643609307018No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGTA1530.24118796897660633No Hit
TACAGAGGTGGCGAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA1360.21438930575698342No Hit
TACGTAGGGTGCGAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1330.209660129894697No Hit
TACGGAGGGTGCAAGCGNTAATCGGAATTACTGGGCGTAAAGCGCACGTA1270.20020177817012424No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA1260.19862538621602874No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1180.186014250583265No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA1100.1734031149505013No Hit
TACGGAGGAGGCGAGCGTTGTTCGGAATCATTGGGCGTAAAGGGCTCGTA1090.17182672299640583No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA1070.1686739390882149No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA1070.1686739390882149No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA1070.1686739390882149No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA1070.1686739390882149No Hit
TACGGGGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCATGTA1060.16709754713411942No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCACGCA1060.16709754713411942No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGCA1040.1639447632259285No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA1030.162368371271833No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1020.16079197931773756No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA960.15133362759316477No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA920.1450280597767829No Hit
TACGGAGGATCCGAGCGNTATCCGGATTTATTGGGTTTAAAGGGTGCGTA920.1450280597767829No Hit
TACGTAGGTGGCAAGCGNTATCCGGAATTATTGGGCGTAAAGGGTGCGTA870.13714610000630556No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA840.13241692414401918No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATCACTGGGCGTAAAGGGAGCGTA830.1308405321899237No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA830.1308405321899237No Hit
TACGTAGGGGACAAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGCA820.12926414023582822No Hit
TACGTAGGGGGCAAGCGNTATCCGGAATTACTGGGCGTAAAGGGTGCGTA800.1261113563276373No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA800.1261113563276373No Hit
TACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA800.1261113563276373No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA790.12453496437354185No Hit
TACGTAGGTGGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGTGCGCA790.12453496437354185No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGTGTGTA770.1213821804653509No Hit
TACGTAGGGGACAAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA770.1213821804653509No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA760.11980578851125544No Hit
TACGAAGGTCCCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGAGCGTA750.11822939655715997No Hit
TTCCAGCTCTAATAGCGTATACTAAAGTTGCTGCAGTTAAAAAGCTCGTA730.11507661264896904No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA710.1119238287407781No Hit
TACGTAGGGTGCAAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA690.10877104483258719No Hit
GACGTAGGTGGCAAGCGTTACTCGGAATTACTAGGCGTAAAGCGCGCGTA670.10561826092439625No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA660.10404186897030078No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA660.10404186897030078No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA650.10246547701620531No Hit
TACAGAGGGTGCAAGCGTTAATCGGAGTTACTGGGCGTAAAGGGCGCGTA640.10088908506210985No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGTGCGTA640.10088908506210985No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCACGCA640.10088908506210985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCC2950.0244.790246
GAGGTCT100.0014767055244.790225
GCGACCG100.0014767055244.79022245
GCGATCG100.0014767055244.79022245
GGTCTCA100.0014767055244.790227
ATGGTGC100.0014767055244.790226
GGATCCA100.0014767055244.790227
GTCCCGA350.0244.790228
ACGAGTG100.0014767055244.790222
TACGTAA100.0014767055244.790221
GACGTAG100.0014767055244.790221
GATCCAA100.0014767055244.790228
CGAATGG100.0014767055244.790223
CGAACAG350.0244.79022245
TACGAGT100.0014767055244.790221
TACGAAT100.0014767055244.790221
TACGAAG1300.0244.790221
AGGTCTC100.0014767055244.790226
AGGTCCC400.0244.790226
GTAGGGT6850.0244.790224
Produced by FastQC (version 0.11.5)